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EC number: 848-537-7 | CAS number: 1912392-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany
- Preparation of inoculum for exposure: the sludge was sieved, left alone to settle and the supernatant was decanted. The sludge was washed one time with isotonic solution. A small amount of the sludge was weighed and dried. From this result a stock solution of activated sludge in tap water was prepared with a mixed liquor suspended solids level of about 1 – 4 g dry mass sludge/liter.
The sludge stock solution was fed with synthetic sewage feed and was incubated aerated overnight at 20 ± 2 °C in the dark. The amount of food applied was 25 mL/L. After this preincubation, the activated sludge was adjusted on a mixed liquor suspended solids level of about 2 – 4 g dry mass sludge/liter. From this sludge, an amount to achieve a mixed liquor suspended solids level in the test vessel of nominally 1.5 g/L was applied. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.5 - 21.5 °C
- pH:
- 8.4 - 8.5
- Nominal and measured concentrations:
- Nominal concentrations: 62.5, 125, 250, 500 and 1000 mg Pt TEAH solution per liter
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L Erlenmeyer flasks
- Material, size, headspace, fill volume: 500 mL
- Aeration: air flow 0.5 – 1 L/min
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6 vessels containing only inoculum (3 replicates were measured at the beginning and 3 at the end of the test assay series.
- No. of vessels per positive control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 3.8 g/L
- Nutrients provided for bacteria: synthetic sewagecontainin peptone 16.0 g, meats extract 11.0 g, urea 3.0 g, NaCl 0.7 g, CaCl2.2H2O 0.4 g, MgSO4.7H2O 0.2 g, K2HPO4 2.8 g and deionised water ad 1000 mL.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water, free from inhibitory concentrations of toxic substances (e.g. Cu2+ ions)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : the total microbial respiration rate (total oxygen uptake including heterotrophic and nitrification uptake) was investigated by measurement of the oxygen concentration over a period of 10 minutes or at least until an O2-content of 2.5 mg O2/L was reached 3 hours after start of incubation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: A range finding test was conducted to check if any adverse effect occurs up to and including a nominal loading of 1000 mg/L (test concentrations 10, 100 and 1000 mg/L). The effect of the test item on the total microbial respiration rate was investigated. Inhibition was found to be greater than 20% at 1000 mg/L. Therefore, a dose-response test with five concentrations was applied as main test.
- Test concentrations: 62.5, 125, 250, 500 and 1000 mg/L test item (definite test)
- Results used to determine the conditions for the definitive study:significant inhibition was observed in the range finding study - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 145 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other:
- Remarks:
- 95% Confidence Interval: 43 - 239 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other:
- Remarks:
- 95% Confidence Interval: >1000 - >1000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The EC50 for the reference item was found to be 15.7 mg/L and is therefore within the accepted range of the OECD 209 guideline of 2 to 25 mg/L.
- Reported statistics and error estimates:
- A LOEC was calculated by using ANOVA followed by Step-down Jonckheere-Terpstra Test Procedure. For trend analysis, a Contrasts test (Monotonicity of Concentration/Response) for metric data was applied. Since the test results show a concentration-response relationship, they were statistically analysed to determine EC values together with 95% confidence intervals using Probit-analysis assuming log-normal distribution of the values a non-linear data analysis.
The evaluation of the concentration-effect-relationships and the calculations of effect concentrations were based on the nominal test item concentrations.
For statistical calculations, the computer program ToxRat Professional version 3.3.0 by ToxRat® Solutions GmbH was applied. - Validity criteria fulfilled:
- yes
- Conclusions:
- A significant inhibition of bacterial respiration by Pt TEAH solution started at a concentration of 500 mg per liter nominal loading. The NOEC is 250 mg Pt TEAH solution per liter. The EC10 and EC50 of Pt TEAH solution is 145 mg/L (95% CL: 43 – 239 mg/L) and >1000 mg/L (95% CL: >1000 – >1000), respectively, based on nominal loading.
- Executive summary:
An activated sludge respiration inhibition test was conducted with di(tetraethylammonium)hexahydroxoplatinate(IV). The study is reliable without restrictions, being GLP-compliant and having followed standard test guidelines (OECD 209 and EU Method C.11).
The test item was given to the synthetic sewage with a concentration of 62.5, 125, 250, 500 and 1000 mg Pt TEAH solution per liter. A positive control was used, and all validity criteria were met. Di(tetraethylammonium)hexahydroxoplatinate(IV) had a statistically significant effect on bacterial respiration. Starting at a loading of 500 mg/L, the test item inhibited the respiration of the inoculum significantly. The NOEC was found to be 250 mg test item per liter (nominal loading).The EC10and EC50of di(tetraethylammonium)hexahydroxoplatinate(IV) is 145 mg/L (95% CL: 43 – 239 mg/L) and >1000 mg/L (95% CL: >1000 – >1000), respectively, based on nominal loading.
Reference
Mean respiration rate (mg O2/(h*g dry mass)).
TI = Test item; SD = Standard deviation; CV = Coefficient of variance.
|
Control |
62.5 mg TI / L |
125 mg TI / L |
250 mg TI / L |
500 mg TI / L |
1000 mg TI / L |
Mean |
36.5 |
39.7 |
38.7 |
31.7 |
24.7 |
26.4 |
SD |
8.7 |
8.6 |
10.0 |
6.0 |
3.2 |
2.1 |
CV |
23.7 |
21.5 |
25.7 |
19.1 |
13.1 |
8.1 |
Mean inhibition (%) of the respiration rate by the test item.
TI = Test item; *: significant difference when compared with control (p < 0.05).
62.5 mg TI / L |
125 mg TI / L |
250 mg TI / L |
500 mg TI / L |
1000 mg TI / L |
-8.9% |
-6.0% |
13.2% |
32.3% * |
27.7% * |
Effect concentration values [mg/L] for respiration rate.
EC = Effect concentration where x % inhibition deviation occurred against control; CL = Confidence level.
Parameter |
EC10 |
EC20 |
EC50 |
EC80 |
NOEC |
LOEC |
||||
|
[mg test item/L] |
|||||||||
Value |
145 |
396 |
>1000 |
>1000 |
250 |
500 |
||||
95 %-CL |
(43 – 239) |
(241 – 573) |
(>1000 – >1000) |
(>1000 – >1000) |
- |
- |
Description of key information
A significant inhibition of bacterial respiration by di(tetraethylammonium)hexahydroxoplatinate(IV) started at a concentration of 500 mg per liter nominal loading. The NOEC is 250 mg test item per liter. The EC10 and EC50 of the test item is 145 mg/L (95% CL: 43 – 239 mg/L) and >1000 mg/L (95% CL: >1000 – >1000), respectively, based on nominal loading.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
An activated sludge respiration inhibition test was conducted with di(tetraethylammonium)hexahydroxoplatinate(IV). The study is reliable without restrictions, being GLP-compliant and having followed standard test guidelines (OECD 209 and EU Method C.11).
The test item was given to the synthetic sewage with a concentration of 62.5, 125, 250, 500 and 1000 mg Pt TEAH solution per liter. A positive control was used, and all validity criteria were met. Di(tetraethylammonium)hexahydroxoplatinate(IV) had a statistically significant effect on bacterial respiration. Starting at a loading of 500 mg/L, the test item inhibited the respiration of the inoculum significantly. The NOEC was found to be 250 mg test item per liter (nominal loading).The EC10and EC50of di(tetraethylammonium)hexahydroxoplatinate(IV) is 145 mg/L (95% CL: 43 – 239 mg/L) and >1000 mg/L (95% CL: >1000 – >1000), respectively, based on nominal loading.
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