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EC number: 949-986-2 | CAS number: 49720-39-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-09-30 to 1992-05-20
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Arginine
- EC Number:
- 200-811-1
- EC Name:
- Arginine
- Cas Number:
- 74-79-3
- Molecular formula:
- C6H14N4O2
- IUPAC Name:
- arginine
- Test material form:
- not specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was weighed out and each portion was dissolved in 1 l of dilution
water. 10 g were dissolved in 1 l for the initial limit test as well as for the full test. For the latter test aliquots were diluted to 500 ml dilution water.
- Eluate: The dilution water used was DSWL, prepared from groundwater.
- Controls: DSWL (negative control)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): visually
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: daphnia, water flea
- Source: TNO
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: according to principles of NPR 6503 - Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Hardness:
- Hardness, expressed as CaCO3: 204 mg/l.
- Test temperature:
- 20°C +/- 1°C
- pH:
- All data are for the full test.
Control medium: Beginning of test: 7.9. End of test: 7.7
1 g/l test substance: Beginning of test: 9.4. End of test: 8.9
1.8 g/l test substance: Beginning of test: 9.7. End of test: 9.0
3.2 g/l test substance: Beginning of test: 9.9. End of test: 9.1 to 9.2 5.6 g/l test substance: Beginning of test: 10.0. End of test: 9.3 to 9.4 10 g/l test substance: Beginning of test: 10.3. End of test: 9.6 - Dissolved oxygen:
- All data are for the full test.
Control medium: Beginning of test: 8.5 mg/l. End of test: 7.6 to 8.1 mg/l.
1 g/l test substance: Beginning of test: 8.8 mg/l. End of test: 8.2 mg/l.
1.8 g/l test substance: Beginning of test: 8.7 mg/l. End of test: 8.3 to 8.4 mg/l. 3.2 g/l test substance: Beginning of test: 8.9 mg/l. End of test: 8.4 to 8.5 mg/l. 5.6 g/l test substance: Beginning of test: 8.7 mg/l. End of test: 8.3 to 8.5 mg/l. 10 g/l test substance: Beginning of test: 8.7 mg/l. End of test: 8.4 to 8.5 mg/l - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Initial limit test: Nominal concentration of test substance: 10 g/l
Full test: Nominal concentrations of test substance: 1.0, 1.8, 3.2, 5.6, 10 g/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: all-glass beakers
- Type: open
- Material, size, fill volume: glass, 150 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Groundwater from Linschoten, the Netherlands. Medium prepared by adding several salts.
- Total organic carbon: 1.5 mg/l
- Metals: << 1 mg/l
- Chlorine: 2.71 mml/l
- Na+: 1.19 mmol/l
- K+: 0.22 mmol/l
- Ca 2+: 1.30 mmol/l
- Mg2+. 0.74 mmol/l
- SO42-: 0.69 mmol/l
- Ca/mg ratio: 1.76
- Intervals of water quality measurement: Once prior to beginning for the above parameters
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light - 8 h dark, with transition periods of 30 minutes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50, EC100, NOEC (mobility = 24h EC0), NOEC (condition) after 24 h.
Condition: Swimming behaviour, colour, or any other morphological or visual behavioural criterion after 24 h.
TEST CONCENTRATIONS
- Test concentrations: 1.0, 1.8, 3.2, 5.6, 10 mg/l
TEST CONCENTRATION INITIAL LIMIT TEST
- Range finding study: yes
- Test concentrations: 10 mg/l
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8
- Results used to determine the conditions for the definitive study: lower concentrations and concentration of limit test
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.8 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval: 1.5 - 2.1 g/l
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 5.6 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Remarks:
- (mobility)
- Effect conc.:
- 1 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- (= 24h EC0)
- Remarks on result:
- other: The NOEC values were estimated by comparing effects with regard to mobility, according to the definition given in OECD 202 (ref. 1) and condition of the exposed animals with those of the control animals.
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Remarks:
- (condition)
- Effect conc.:
- < 1 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Swimming behaviour, colour, any other visual observable morphological or behavioural criterion
- Remarks on result:
- other: The NOEC values were estimated by comparing effects with regard to mobility, according to the definition given in OECD 202 (ref. 1) and condition of the exposed animals with those of the control animals.
- Details on results:
- n the initial limit test all daphnias were immobile at a concentration of 10 g/l. In the full test there were no immobile daphnias at a concentration of 1.0 g/l, however, at this concentration the daphnias were slower, floating or had particles on their appandages.
It is likely that the results of the test are seriously affected by the high pH value.
- Behavioural abnormalities: At a test concentration of 1.0 g/l some daphnias were slower,
floating or had particles on their appandages. At test concentrations of 1.8 g/l and 3.2g/l some daphnias were slower or floating. At a test concentration of 5.6 g/l some daphnias were floating. - Observations on body length and weight: none
- Other biological observations: none
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test substance appeared to be completely dissolved at all concentrations tested at the beginning of the test (visually assesed). After 24 h undissolved material was
observed on the bottom of the vessel at all concentrations tested.
- Effect concentrations exceeding solubility of substance in test medium: see above
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A limit test according to OECD 202 did not show any adverse effects to Daphnia magna at a test concentration of 1 g/l L-arginine.
A subsequent test with L-arginine according to OECD 202 with higher test concentrations showed the following results:
EC50 = 1.8 g/l; EC100 = 5.6 g/l; NOEC (mobility) was estimated to be 1.0 g/l and NOEC (condition) was estimated to be < 1.0 g/l. - Executive summary:
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