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EC number: 413-390-6 | CAS number: 149144-85-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26.4.1993 - 29.4.1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- According to the OECD-guideline the biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. This corresponds to a specific growth rate of 0.92 day-1. Here the increase is a little bit lower. The number of treatment replicates should be at least six. We have only 3 replicates.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- -Name of test material (as cited in study report): Cosmacol EMI, Butanedioic acid, hydroxy-, di C12-C13-alkyl esters
- Molecular weight (if other than submission substance): 470 - 498
- Substance type: pure active substance
- Physical state: liquid
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: Chlorococalles
- Source (laboratory, culture collection): Institute of Terrestral Ecology Culture Centre of Algae and Protozoa 36 Storey's Way, Cambridge CB3 ODT, England
- Age of inoculum (at test initiation):
- Method of cultivation:
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Study design
- Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 - 24 °C
- pH:
- 7.7-7.9
- Nominal and measured concentrations:
- 100 mg/L dispersion concentration; 1 mg/L water solubility
It was not possible, with the analytical technology available to measure the concentration of the substance in the solution. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass flask
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- Initial cells density: 10000 cells/mL
- Control end cells density: 85000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
GROWTH MEDIUM
- Detailed composition if non-standard medium was used:
OTHER TEST CONDITIONS
- Photoperiod: continuous fluorescent lighting (30 W) - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- other: dispersion concentration (WAF)
- Basis for effect:
- growth rate
- Details on results:
- The difference of the biomass of the control and treated groups is less than 25 %. The difference between the average growth figure of the treated group and control group was 0.56 %.
- Reported statistics and error estimates:
- The percentage difference between the cellular concentration of the group treated and the control group was calculated. The growth curve for both groups was graphically determined and the regression line were calculated. The difference beween the average rate of growth (µ) of treated and control groups was calculated by angular coefficient (b) of regression lines.
Any other information on results incl. tables
time/h | tr. gr. 1/n. cells/mL | tr. Gr. 2/n. cells/mL | tr. Gr.3/n.cells/mL | control 1/n. of cells/mL | control 2/n. cells/mL |
0 | 10000 | 10000 | 10000 | 10000 | 10000 |
24 | 30000 | 30000 | 20000 | 20000 | 30000 |
48 | 60000 | 50000 | 50000 | 50000 | 60000 |
72 | 100000 | 70000 | 90000 | 100000 | 70000 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Executive summary:
The study was conducted on an algal culture of Selenastrum capricornutum in growth phase, at an initial concentration of 10000 cells/ml.
Algae were exposed to the test material, COSMACOL EMI at a concentration (100 mg/L dispersion concentration) exceeding the water solubility (1 mg/L) for 72 hours.
After 24, 48 and 72 hours cell concentration was measured with a Burker chamber.
Under the experimental conditions, no difference in algal growth was seen between treated and control cultures.
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