Registration Dossier
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EC number: 289-064-0 | CAS number: 85959-68-8
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
One acute toxicity study with earthworms is available for EDTA-FeNa (Winkelmann, 2019). The EC50 for weight was determined to be 129 mg a.i./kg dw. The active ingredient content of the test item was 100 %.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 129 mg/kg soil dw
Additional information
The acute effects of EDTA-FeNa on the earthworm Eisenia fetida were determined according to OECD Guideline 207 under GLP conditions. The study was conducted under static conditions for 14 days with the test item concentrations 3.16 – 10 – 31.6 – 100 – 316 mg/kg soil dry weight mixed into artificial soil containing 10 % peat. Artificial soil without test item was used as control. 40 test organisms with an individual weight between 0.40 - 0.54 g, divided into four replicates, were tested per test item concentration and control.
A statistically significant mortality was observed in the test item concentrations 100 and 316 mg/kg soil dry weight after 14 days of exposure, whereas no mortalities were observed in the control and the test item concentrations 3.16 to 31.6 mg/kg soil dry weight.Consequently, the NOEC was determined to be 31.6 mg/kg soil dry weight and LOEC was determined to be 100 mg/kg soil dry weight. In terms of body weight loss, the NOEC was determined to be 31.6 mg/kg soil dry weight and LOEC was determined to be 100 mg/kg soil dry weight. The EC50 for weight was determined to be 129 mg a.i./kg dw. The active ingredient content of the test item was 100 %.
Summary of all Effects
Effects |
Dissolvine E-Fe-13 [mg/kg soil dry weight] |
LOEC Mortality, behaviour, pathological symptoms |
100 |
NOEC Mortality, behaviour, pathological symptoms |
31.6 |
LOEC Change of body weight |
100 |
NOEC Change of body weight |
31.6 |
LC10mortality(confidence interval) LC20mortality(confidence interval) LC50mortality(confidence interval)
|
56.8 (7.84 - 95.9) 76.1 (18.3 – 123) 133 (70.1 - 262)
|
EC10body weight(confidence interval) EC20body weight(confidence interval) EC50body weight(confidence interval)
|
51.3 (38.3 – 68.7) 70.4 (52.9 – 93.8) 129 (88.3 – 185)
|
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