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EC number: 278-188-0 | CAS number: 75314-26-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20-21 June 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Further details on test item are lacking.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- other: "Proposed Method for the Determination of the Biodegradability of Surfactants used in Synthetic Detergents" (OECD Technical Report of June 11, 1976).
- Version / remarks:
- June 11, 1976
- GLP compliance:
- no
- Specific details on test material used for the study:
- Storage: At room temperature, in the dark
- Details on sampling:
- After 180 minutes of aeration, the first sample was taken for oxygen measurement over a period of up to 10 minutes. Thereafter, in 15 minutes intervals,
the rest of the samples were submitted to measurement of oxygen concentration. - Vehicle:
- no
- Details on test solutions:
- - Test substance: 500 mg/l in distilled water
- Controls: 3,5-Dichlorophenol. 0.5 g in 10 ml 1N NaOH, dilurte to 30 ml with distilled water, neutralized with 1N H2SO4 and diluted to 1 L. Final pH: 7.9. Final concentration 500mg/l - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: domestic waste-water treatment plant (ARA, Sissach/Switzerland)
- Preparation of inoculum for exposure: The sludge was coarse sieved, centrifuged, the supernatant liquid phase decanted, the solid material resuspended in tap water and again centrifuged. This procedure was repeated further 2 times. A volume of of this washed sludge suspension corresponding to 4.0 g dry material was made up with Soerensen buffer (pH 7) to 1 L. To that mixture 50 ml OECD recommended synthetic sewage feed were added. Thereafter, the sludge was incubated overnigth at 20-25 °C under aereation.
- Initial biomass concentration: 4.0 g dry weight/l - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- pH:
- 7.8
- Nominal and measured concentrations:
- Nominal: 10 and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass-flasks (>500 ml)
- Fill volume: 500 ml
- Aeration: 0.7 l/min
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per positive control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 4.0 g/l
OTHER TEST CONDITIONS
- Adjustment of pH: No
TEST CONCENTRATIONS
- Test concentrations: 10 and 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol, 3.2, 10 and 50 mg/l
- Key result
- Duration:
- 180 min
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate: 0.36 +/- 0.02 mg O2/l min
- Results with reference substance (positive control):
- - Results with reference substance valid? yes IC50 should be 5-30 mg/l
- Relevant effect levels: IC 50: 8 mg/l - Validity criteria fulfilled:
- yes
- Conclusions:
- The tests are regarded to be valid since the variance between the control samples was 5.6 %. Furthermore, it was shown that the inhibitory effect of 3,5-dichlorophenol was with 8 mg/l wlthin the range of the IC 50-value of 5 to 30 mg/l. The test item showed, under the same conditions, an IC 50-value of >=100 mg/l .
In conclusion, the test item was found to be non-toxic to waste-water bacteria. - Executive summary:
The inhìbitory effect of the test item on aerobic waste-water bacteria was investigated in a respiration test.
The test article showed no inhibition (-9.9 %) of the respiration rate up to a concentration of 100 mg/l.
The IC 50 was determined to be:
IC 50:>100 mg/l.
In conclusion, the test item was found to be non-toxic to waste-water bacteria.
The IC 50 for dichlorophenol tested under the same conditions was about 8 mg/l.
Reference
Chemical | Concentration (mg/l) | Oxygen consumption (mg O2/l min) | Inhibiton (%) |
Control | 0 | 0.34 |
|
Control | 0 | 0.37 | |
3,5-dichlorophenol | 50 | 0.03 | 91.6 |
3,5-dichlorophenol | 10 | 0.18 | 49.3 |
3,5-dichlorophenol | 3.2 | 0.25 | 29.6 |
Test item | 100 | 0.39 | -9.9 |
Test item | 10 | 0.38 | -7.0 |
Description of key information
The tests are regarded to be valid since the variance between the control samples was 5.6 %. Furthermore, it was shown that the inhibitory effect of 3,5-dichlorophenol was with 8 mg/l wlthin the range of the IC 50-value of 5 to 30 mg/l. The test item showed, under the same conditions, an IC 50-value of >=100 mg/l .
In conclusion, the test item was found to be non-toxic to waste-water bacteria.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
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