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EC number: 269-503-2 | CAS number: 68259-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Reported 12Jun1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate
- EC Number:
- 269-503-2
- EC Name:
- Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate
- Cas Number:
- 68259-00-7
- Molecular formula:
- C14H16N3.CH3O4S
- IUPAC Name:
- methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate
- Test material form:
- solid: particulate/powder
- Details on test material:
- See study reports for batch and purity information
Constituent 1
- Specific details on test material used for the study:
- Purity ca 90%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/1, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/1, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.
During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The organism used in this test system is the species Daphnia magna. The Daphnia were bred in the laboratories of the Product Ecology TF, Textile Dyes Division under standardised conditions.
Breeding :
Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22''C. The water was renewed partially three times per week. The Daphnia were fed with a suspension of green algae {Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantities that the food was consumed within 24h.
Pre-treatment:
24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 1000 µm sieve.
For each test concentration, 20 Daphnia divided in two groups of 10 animals were used.
Study design
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Reference substance (positive control):
- yes
- Remarks:
- The quality of the Daphnia will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1.04 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.9 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1.04 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.04 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 (issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).
The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.
The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/L, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.
During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test.
No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 1.04 mg/l, the EC100 value was 4.4 mg/I. - Results with reference substance (positive control):
- The quality of the Daphnia will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l).
Last reference control at January 25, 1995 EC50 : 0.95 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 (issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).
The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/L, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.
During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test.
No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 1.04 mg/l, the EC100 value was 4.4 mg/I. - Executive summary:
The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 (issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).
The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.
The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/L, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.
During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test.
No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 1.04 mg/l, the EC100 value was 4.4 mg/I.
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