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EC number: 269-246-6 | CAS number: 68201-95-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 23rd, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted Aprll 4, 1984
- GLP compliance:
- no
- Details on test solutions:
- The test article was dissolved in distilled water at a concentration og 500 mg/l.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: the tests were run with aeroblc actìvated sludge from a domestic waste-water treatment plant (ARA, Sissach/Switzerland).
- Pretreatment: the sludge was coarsely sieved, centrifuged, the supernatant liquld phase decanted, the solid material resuspended in tap water and again centrlfuged. This procedure was repeated a further two times.
- Preparation of inoculum for exposure: an aliquot of the flnal sludge suspension was weighed, dried and the ratio of wet sludge (ml) to its dry weight (g) determined. Based on this ratio, a volume of washed sludge suspenslon corresponding to 4.0 g dry material were made up with Soerensen buffel (pH 7) to t litre. To that mixture, 50 ml OECD recommended synthetic sewage feed were added. Thereafter, the sludge was incubated overnight at 20 - 25 °C under aeration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 180 min
- Test temperature:
- 22.0 °C
- pH:
- 7.7
- Nominal and measured concentrations:
- 100 and 10 mg/l nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass-flasks (500 ml).
- Aeration: flasks were aerated with 0.2 l/minute.
- No. of organisms per vessel: 200 ml of activated sludge.
- No. of vessels per concentration: one replicate.
- No. of vessels per control: two replicates.
- No. of vessels per vehicle control: three concentrations tested (i.e. 50, 10 and 3.2 mg/l); one replicate each one.
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to the OECD guideline.
EFFECT PARAMETERS MEASURED
- Parameter: oxygen consumption.
- Sampling: after 180 minutes of aeration at 22.0 °C, the first sample was taken for oxygen measurement over a period of up to 10 minutes. Thereafter, in 15 minutes intervals, the rest of the samples were submitted to measurement of oxygen concentration.
Sampling was started with the control 1. Thereafter, the measurements were continued with the positive controls and the test article samples. Finally,
the oxygen consunption of control 2 was deternined.
- Measurements: oxygen consumption was measured over a period of 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. The apparatus used for oxygen meesurements was the WTW OXI 91 (Wissenschaftlich-Technische Werkstaetten, Weilheim/Federal Republìc of Gernany). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol sodium salt (500 mg/l)
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The aeration of the test medium with test article caused the solutions to foam. The foam was coloured. Under these conditions, the respiration rate of the sludge was not inhibited (0 %) at the highest concentration of the test substance of 100 mg/l.
- Results with reference substance (positive control):
- - Results with reference substance: valid.
- Relevant effect levels: IC50 was found to be 10.6 mg/l. Almost complete inhibition (93.6 %) was observed at 50 mg/l. - Validity criteria fulfilled:
- yes
- Remarks:
- the two controls respiration rated were withing 15 % of each other and the IC50 (3h) of 3,5-dichlorophanol resulted to be in the accepted range 5 to 30 mg/l
- Conclusions:
- IC50 (3h) > 100 mg/l (nominal)
- Executive summary:
Acute toxicity of the test substance to microorganisms was examined according to OECD guideline 209. This method determines the respiration rate (oxygen consumption) of an aerobic activated sludge. Aerobic activated sludge from a domestic waste-water treatment plant was used in the test. Two blank controls were used and 3,5 -dichlorophenol was employed as the reference standard. Nominal test substance concentrations were 100 and 10 mg/l.
Percentage inhibition by reference standards and test concentrations were determined as a function of mean oxygen consumption of the blank controls. The aeration of the test medium with test article caused the solutions to foam. The foam was coloured. Under these conditions, the respiration rate of the sludge was not inhibited (0 %) at the highest concentration of the test substance of 100 mg/l.
Conclusion
IC50 (3h) > 100 mg/l (nominal)
Reference
Influence of the test substance on oxygen consumption of activated sludge
Concentration [mg/l] |
Oxygen consumption [mg O2/ l min] |
Inhibition [%] |
Starting pH | |
Control | - | 0.46 | - | 7.7 |
Control | - | 0.48 | - | 7.7 |
Mean± s | 0.47 ± 0.01 | 4.3 | - | |
Test item | 100 | 0.47 | 0 | 7.7 |
Test item | 10 | 0.48 | -2.1 | 7.7 |
3,5-dichlorophenol | 50 | 0.03 | 93.6 | 7.7 |
3,5-dichlorophenol | 10 | 0.28 | 40.4 | 7.7 |
3,5-dichlorophenol | 3.2 | 0.36 | 23.4 | 7.7 |
Description of key information
Not harmful/toxic for microorganisms.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
Acute toxicity of the test substance to microorganisms was examined according to OECD guideline 209. This method determines the respiration rate (oxygen consumption) of an aerobic activated sludge. Aerobic activated sludge from a domestic waste-water treatment plant was used in the test. Two blank controls were used and 3,5 -dichlorophenol was employed as the reference standard. Nominal test substance concentrations were 100 and 10 mg/l.
Percentage inhibition by reference standards and test concentrations were determined as a function of mean oxygen consumption of the blank controls. The aeration of the test medium with test article caused the solutions to foam. The foam was coloured. Under these conditions, the respiration rate of the sludge was not inhibited (0 %) at the highest concentration of the test substance of 100 mg/l (Ritter, 1989).
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