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EC number: 241-155-6 | CAS number: 17090-93-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 23 March 2006
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- sodium 2-amino-3-carboxypropanoate
- Cas Number:
- 323194-76-9
- Molecular formula:
- C4H7NO4.xNa
- IUPAC Name:
- sodium 2-amino-3-carboxypropanoate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Name: L-aspartic acid, sodium salt monohydrate 1
Batch/Lot number: Z201125/ VG29848563
CAS number: 323194-76-9
Anhydrous substance name: L-aspartic acid, sodium salt, CAS: 17090-93-6
Appearance: Solid, white, crystalline powder
Purity: 98%
Expiry date: 24 November 2022
Storage conditions: Room temperature (15-25 ºC), protected from humidity (tight closed
container)
Constituent 1
- Specific details on test material used for the study:
- Information supplied by the Sponsor:
Name: L-aspartic acid sodium salt monohydrate
Lot number: Z201125 / VG29848563
CAS number: 323194-76-9
Description: Solid, crystalline powder, white
Expiry date: 24 November 2022
Purity: min. 98 %
Storage conditions: Room temperature (15-25ºC, below 70% relative humidity)
Safety precautions: Routine safety precautions (lab coat, gloves, safety glasses, face mask) for unknown materials will be applied to assure personnel health and safety.
The certificate of analysis is presented in Appendix 3.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For determination of the test item concentration, samples were taken from the test solutions and the controls at the start, at 24, 48 hours
and at the end of the experiment. Samples were transferred immediately after collection for
concentration measurement to the Test Facility.
The concentration analyses were conducted under the control of the Analyst, based on the analytical
method validation and in compliance with the relevant SOPs of the Test Facility. The results are
included in the Study Report as Appendix 2.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared just before inoculation with the algae (start of the experiment) by dissolving 0.0702g of test material in 700 mL OECD medium. The test solutions were prepared by diluting the stock solution.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Raphidocelis subcapitata
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 +-1 °C.
- pH:
- The pH was measured in the control and in the highest test concentration at the start and in each of these test vessels at the end of the experiment. The pH varied by not more than 1.5 units of the controls.
- Details on test conditions:
- The vessels were held for the duration of the study in a climate chamber, the test temperature was measured continuously with a min/max thermometer and remained between 21.3 and 22.8 °C. The pH was measured in the control and in the highest test concentration at the start and in each of these test vessels at the end of the experiment. The pH varied by not more than 1.5 units of the controls.
Illumination: fluorescent lamps were used with continuous lighting with light intensity of
6941 – 8128 lux. - Reference substance (positive control):
- yes
- Remarks:
- For the evaluation of the quality of the algal cultures and the experimental conditions, potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- In the 72-h algal growth inhibition test on Raphidocelis subcapitata, the 72-h EC50 value based on growth rate was determined to be higher than 100 mg/L and based on yield was calculated to be higher than 100 mg/L. The NOEC related to growth rates was determined to be 100 mg/L and related to yield it was determined to be 100 mg/L.
The results are based on the nominal concentration.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the 72-h algal growth inhibition test on Raphidocelis subcapitata, the 72-h EC50 value based on growth rate was determined to be higher than 100 mg/L and based on yield was calculated to be higher than 100 mg/L. The NOEC related to growth rates was determined to be 100 mg/L and related to yield it was determined to be 100 mg/L.
The results are based on the nominal concentration. - Executive summary:
The purpose of this study was to determine the effect of the test item on the growth of a unicellular green algal species Raphidocelis subcapitata. Exponentially growing cultures of Raphidocelis subcapitata were exposed to the test item over several generations under defined conditions.
As slight inhibition was seen in the highest concentration in the preliminary study, a full test with 5 test concentrations was carried out in the main test. The test design included three replicates of the test concentrations and six replicates for the untreated control.
As the test item was expected not to be stable for 72 hours, analytical measurements were carried out daily. However the concentration decreased, the final values were over 80% of the start concentration in the higher doses. As all the EC values are over the highest dose, all biological results are based on nominal concentrations.
The initial biomass in the test cultures was set to 104 cells/mL.
Glass flasks with total capacity of ~250 mL were used as test vessels. The volume of the test liquid in the vessels was 100 mL. The test vessels were covered with air-permeable stoppers.
Table 1. Summary of Biological Endpoints
Parameter
Growth rate (r)
[mg/L]
Yield (y)
[mg/L]
72-h EC50
>100
>100
72-h EC100
>100
>100
72-h NOEC
100
100
Conclusion: In the 72-h algal growth inhibition test on Raphidocelis subcapitata, the 72-h EC50 value based on growth rate was determined to be higher than 100 mg/L and based on yield was calculated to be higher than 100 mg/L. The NOEC related to growth rates was determined to be 100 mg/L and related to yield it was determined to be 100 mg/L.
The results are based on the nominal concentration.
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