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EC number: 240-834-4 | CAS number: 16803-97-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- Data is from study report.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15)
- Principles of method if other than guideline:
- Short term toxicity to Leuciscus idus study was carried out for 96 hrs using Standard Operating Procedures (SOP) of the laboratory of fish toxicology. The study was performed according to DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15), respectively.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material : 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide
- Common name: Diaminodiphenylsulfamid
- Solubility in water: slightly soluble
- Test substance no.: 87/135
- Molecular formula : C12H13N3O2S
- Molecular weight : 263.32 g/mol
- Smiles notation : O=S(=O)(c1ccc(cc1)Nc1ccc(cc1)N)N
- InChl : 1S/C12H13N3O2S/c13-9-1-3-10(4-2-9)15-11-5-7-12(8-6-11)18(14,16)17/h1-8,15H,13H2,(H2,14,16,17)
- Substance type : Organic
- Physical state : Solid - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: Test chemical concentration used for the study were 250 and 500 mg/l, respectively.
- Sampling method: The product/test chemical was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria. - Vehicle:
- not specified
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden Orfe
- Length at study initiation (length definition, mean, range and SD): 5.1 cm (ranges from 4.8 to 5.7 cm)
- Weight at study initiation (mean and range, SD): 1.8 gm (ranges from 1.4 to 2.7 gm)
- Housing: The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Duration of housing and adaptation: about 2 weeks
ACCLIMATION
- Acclimation period: about 2 weeks
- Type and amount of food during acclimation: Growth feed F/B 50, Ad libitum
- Adaptation to test water and test temperature: 3 days - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- Total hardness: about 2.5 mmol/l
- Test temperature:
- 20 ± 1°C
- pH:
- About 7.6
- Dissolved oxygen:
- Oxygen content: > 60% of maximum saturation
- Nominal and measured concentrations:
- Nominal concentrations: 250 and 500 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass aquarium
- Material, size, headspace, fill volume: The size of the test vessel was 30 cm X 22 cm X 24 cm, volume 10 ml
- Aeration: Yes, slight aeration was given.
- No. of organisms per vessel: 10/conc.
- Biomass loading rate: 1.8 fish/l test water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according to Din 38 412, part 11, draft sept . 1981 preparation from fully demineralized tap water
Conductivity : max . 10 micro mho
The resalting is carried out by addition of
294.0 MG/L CaCl2 . 2 H20
123.3 MG/L MgSO4 . 7 H20
64.8 MG/L NaHC03
5.8 MG/L KCl
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Acid capacity: 0.8 mmol/l
OTHER TEST CONDITIONS
- Photoperiod: 16 hr light and 8 hr darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Determination of mortality and symptoms; Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC 5 and the LC 95 using the probit analysis* after hours (nominal conc .) : 1, 24, 48, 72, 96
TEST CONCENTRATIONS
- Range finding study: Yes, based on the results of a range finding study (lC 50 after 96 h : > 500 mg/l) and according to the highest concentration of 500 mg/l, nowadays generally accepted for acute studies using the din-method, and to be sure to obtain the NOEC, the concentrations were fixed as follows : 250 and 500 mg/l, respectively.
- Test concentrations: Test chemical concentration used for the study were 250 and 500 mg/l, respectively. - Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide was used as a positive control
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No abnormalities were observed in any of the test animals after a period of 96 hrs.
No observable effect concentration: 500 mg/l
Maximum concentration causing no mortality: 500 mg/l
Minimum concentration causing 100$ mortality: > 500 mg/l - Reported statistics and error estimates:
- Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC 5 and the LC 95 using the probit analysis* after hours (nominal conc .) : 1, 24, 48, 72, 96
- Sublethal observations / clinical signs:
Positive control of animals conducted with chloroacetamide LC50 after 48 h: about 32 mg/l. This lethal concentration corresponds to the normal sensitivity.
Mortality during the time of housing: about 0 .1%
Mortality during the adaptation period: 0%
Corpulence factor of the batch*: 1 .4
The corpulence factor K is calculated from the weight w(g) and the length l(cm) measured from the tip of the mouth to the end of the tail fin according to the formula k=100*w/l**3 .
Results
Nominal concentration (mg/l)
Number of fish
Dead fish after
1h
4h
24h
48h
72h
96h
250
500
0
10
10
10
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Nominal concentration (mg/l)
Symptoms
1h
1h
1h
1h
1h
1h
250
500
0
No symptoms detectable
Explanation of Symptoms:
A = Apathy
E = Exophthalmos
H = Hyperreflexia
L = Gasping
T = Tumbling
V = Discoloration
X = Accelerated respiration
B = Abdominal distension
F = Escape reflex
K = Convulsions
N = Narcotic-like state
U = Restlessness
W = Headstand
Nominal concentration (mg/l)
pH
Beginning
4h
24h
48h
72h
96h
250
500
0
7.7
7.7
7.8
7.7
7.8
7.7
7.8
7.8
7.7
7.8
7.8
7.7
7.8
7.8
7.7
Nominal concentration (mg/l)
Oxygen content (mg/l)
Beginning
4h
24h
48h
72h
96h
250
500
0
8.8
8.8
8.5
8.3
8.4
7.9
8.3
8.6
7.6
8.4
8.5
8.0
8.7
8.7
8.1
Nominal concentration (mg/l)
Temperature (°C)
Beginning
4h
24h
48h
72h
96h
250
500
0
20
20
20
20
20
20
21
20
21
20
20
21
20
20
20
Nominal Concentrations
Fish Toxicity: After 1h
Concentration (mg/l)
Number of fish start
Dead fish after 1h
Mortality (%)
Concentration used
250
500
10
10
0
0
0.0
0.0
*
LC50 ˃ 500 (1% significance level)
Fish Toxicity: After 24h
Concentration (mg/l)
Number of fish start
Dead fish after 24h
Mortality (%)
Concentration used
250
500
10
10
0
0
0.0
0.0
*
LC50 ˃ 500 (1% significance level)
Fish Toxicity: After 48h
Concentration (mg/l)
Number of fish start
Dead fish after 48h
Mortality (%)
Concentration used
250
500
10
10
0
0
0.0
0.0
*
LC50 ˃ 500 (1% significance level)
Fish Toxicity: After 72h
Concentration (mg/l)
Number of fish start
Dead fish after 72h
Mortality (%)
Concentration used
250
500
10
10
0
0
0.0
0.0
*
LC50 ˃ 500 (1% significance level)
Fish Toxicity: After 96h
Concentration (mg/l)
Number of fish start
Dead fish after 96h
Mortality (%)
Concentration used
250
500
10
10
0
0
0.0
0.0
*
LC50 ˃ 500 (1% significance level)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- No abnormalities were observed in any of the test animals after a period of 96 hrs and also no mortality was recorded. Thus, as no adverse effect were observed of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the test organism Leuciscus idus, the NOEC and LC50 value was determined to be 500 and > 500 mg/l, respectively.
- Executive summary:
Short term toxicity to Leuciscus idus study was carried out for 96 hrs using Standard Operating Procedures (SOP) of the laboratory of fish toxicology. The study was performed according to DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15), respectively.The study was based on the effects of the test compound4-amino-N-(4-aminophenyl)benzene-1-sulfonamide(CAS no. 16803-97-7) on Leuciscus idus (Golden Orfe) in a static fresh water system at a temperature of 20 ± 1°C, pH of about 7.6, Total hardness of 2.5 mmol/l and Oxygen content > 60% of maximum saturation with a photoperiod of 16 hr light and 8 hr darkness.Test chemical concentration used for the study were 250 and 500 mg/l, respectively. Chloroacetamide was used as a positive control. Leuciscus idus (Golden Orfe) of 5.1 cm in length (ranges from 4.8 to 5.7 cm), 1.8 gm in weight (ranges from 1.4 to 2.7 gm)was used as a test organism. The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air. The test organism was kept for housing and adapted was about 2 weeks and fed with growth feed F/B 50, Ad libitum. Test was performed in glass aquarium. The size of the test vessel was 30 cm X 22 cm X 24 cm and volume 10 ml. Test system was slightly aerated. No. of organisms used per conc. was 10 and biomass loading rate consists of 1.8 fish/l test water. Reconstituted freshwater water was used as a dilution water during the study. Determination of mortality and symptoms; Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC5 and the LC95 was calculated using the probit analysis after hours 1, 24, 48, 72 and 96, respectively. The 48 hr LC50 for the positive control chloroacetamide was determined to be 32 mg/l. No abnormalities were observed in any of the test animals after a period of 96 hrs and also no mortality was recorded. Thus, as no adverse effect were observed of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the test organism Leuciscus idus, the NOEC and LC50 value was determined to be 500 and > 500 mg/l, respectively. Thus, based on the LC50 value, it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.
Reference
Description of key information
Short term toxicity to Leuciscus idus study was carried out for 96 hrs using Standard Operating Procedures (SOP) of the laboratory of fish toxicology (Sustainability Support Services (Europe) AB, Report no. 10F0135/875091, 1987). The study was performed according to DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15), respectively. The study was based on the effects of the test compound4-amino-N-(4-aminophenyl)benzene-1-sulfonamide(CAS no. 16803-97-7) on Leuciscus idus (Golden Orfe) in a static fresh water system at a temperature of 20 ± 1°C, pH of about 7.6, Total hardness of 2.5 mmol/l and Oxygen content > 60% of maximum saturation with a photoperiod of 16 hr light and 8 hr darkness. Test chemical concentration used for the study were 250 and 500 mg/l, respectively. Chloroacetamide was used as a positive control. Leuciscus idus (Golden Orfe) of 5.1 cm in length (ranges from 4.8 to 5.7 cm), 1.8 gm in weight (ranges from 1.4 to 2.7 gm)was used as a test organism. The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air. The test organism was kept for housing and adapted was about 2 weeks and fed with growth feed F/B 50, Ad libitum. Test was performed in glass aquarium. The size of the test vessel was 30 cm X 22 cm X 24 cm and volume 10 ml. Test system was slightly aerated. No. of organisms used per conc. was 10 and biomass loading rate consists of 1.8 fish/l test water. Reconstituted freshwater water was used as a dilution water during the study. Determination of mortality and symptoms; Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC5 and the LC95 was calculated using the probit analysis after hours 1, 24, 48, 72 and 96, respectively. The 48 hr LC50 for the positive control chloroacetamide was determined to be 32 mg/l. No abnormalities were observed in any of the test animals after a period of 96 hrs and also no mortality was recorded. Thus, as no adverse effect were observed of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the test organism Leuciscus idus, the NOEC and LC50 value was determined to be 500 and > 500 mg/l, respectively. Thus, based on the LC50 value, it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 500 mg/L
Additional information
Short term toxicity to Leuciscus idus study was carried out for 96 hrs using Standard Operating Procedures (SOP) of the laboratory of fish toxicology (Sustainability Support Services (Europe) AB, Report no. 10F0135/875091, 1987). The study was performed according to DIN 38 412 "Test procedure with water organisms (group l) general information for the planning, implementation and evaluation of biological test procedures (L1)" and "Determination of the effect of water ingredients on fish - fish test (L15), respectively. The study was based on the effects of the test compound4-amino-N-(4-aminophenyl)benzene-1-sulfonamide(CAS no. 16803-97-7) on Leuciscus idus (Golden Orfe) in a static fresh water system at a temperature of 20 ± 1°C, pH of about 7.6, Total hardness of 2.5 mmol/l and Oxygen content > 60% of maximum saturation with a photoperiod of 16 hr light and 8 hr darkness. Test chemical concentration used for the study were 250 and 500 mg/l, respectively. Chloroacetamide was used as a positive control. Leuciscus idus (Golden Orfe) of 5.1 cm in length (ranges from 4.8 to 5.7 cm), 1.8 gm in weight (ranges from 1.4 to 2.7 gm)was used as a test organism. The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air. The test organism was kept for housing and adapted was about 2 weeks and fed with growth feed F/B 50, Ad libitum. Test was performed in glass aquarium. The size of the test vessel was 30 cm X 22 cm X 24 cm and volume 10 ml. Test system was slightly aerated. No. of organisms used per conc. was 10 and biomass loading rate consists of 1.8 fish/l test water. Reconstituted freshwater water was used as a dilution water during the study. Determination of mortality and symptoms; Determination or calculation of the median lethal concentration (LC 50) and, if possible, the LC5 and the LC95 was calculated using the probit analysis after hours 1, 24, 48, 72 and 96, respectively. The 48 hr LC50 for the positive control chloroacetamide was determined to be 32 mg/l. No abnormalities were observed in any of the test animals after a period of 96 hrs and also no mortality was recorded. Thus, as no adverse effect were observed of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the test organism Leuciscus idus, the NOEC and LC50 value was determined to be 500 and > 500 mg/l, respectively. Thus, based on the LC50 value, it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.
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