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EC number: 222-376-7 | CAS number: 3452-97-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 2002, Part 2; Daphnia Reproduction Test
- Principles of method if other than guideline:
- GLP guideline study. However, according to OECD TG 202 the exposure concentration selection should be based on the EC50 (24 h) for immobilization, starting at about the 24 h EC50 and ending at about 1/100 of the 24 h EC50. The effects of the test concentrations were far lower.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Regarding the entire test area (1 sample/ 1 test area made by mixing the extracts from the contents of the 4 test containers), after removing the feed by centrifugal separation (at 3000 rpm for 10 minutes) on the exposure start date (0th day), 7th day, 14th day and the exposure end day (21st day), it was analysed by GC by diluting as well as by keeping as-is.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock Solutions Preparations and Stability: A proportional diluter system was used for intermittent introduction of test material solution (0.1 wt% of test chemical with solubilizer hydrogenated caster oil HCO-40 5000mg/L) and dilution and water into the test chambers.
- Controls: water and vehicle controls included
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): hydrogenated caster oil HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 25 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Age at study initiation: < 24 hours after hatching
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- none
- Hardness:
- 55.6mg/L as CaCO3
- Test temperature:
- 19-21 °C
- pH:
- 7.7
- Dissolved oxygen:
- > 60% of saturation
- Nominal and measured concentrations:
- Nominal: 0, 0.128. 0.32, 0.8, 2.0, 5.0 mg/L
Measured: 0, 0.904, 0.2298, 0.641, 1.46, 3.87 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): no data whether open / closed
- Material, size, headspace, fill volume: 1.8 L test solution in 3.0L glass vessel (diameter = 16 cm, depth = 17 cm )
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter
- Renewal rate of test solution (frequency/flow rate): flow rate 50 mL/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: 180 mL/animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Chlorine: <0.02 mg/L
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16h:8h light-dark cycle
- Light intensity: room light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality of parental animals
VEHICLE CONTROL PERFORMED: yes
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 3.87 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- other: ErC50 (reproduction)
- Effect conc.:
- 2.09 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 95% CL: 1.94-2.25 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to OECVDT TG 202 the exposure concentration selection should be based on the EC50 (24 h) for immobilization, starting at about the 24 h EC50 and ending at about 1/100 of the 24 h EC50. The effects of the test concentrations were far lower. As to reproduction, the effect values were:
Reproduction: 21 day EC50 = 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr = 1.46 mg/L; LOECr = 3.87 mg/L - Executive summary:
3, 5,5 -trimethylhexan-1-ol was tested in a Daphnia reproduction test according to OECD TG 202, Part 2, under GLP for 21 days at concentrations up to nominal 5 mg/L (measured 3.87 mg/L). The effect data are as follows:
Mortality: 21 day LC50 > 3.87 mg/L
Reproduction: 21 day ErC50 = 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr = 1.46 mg/L; LOECr = 3.87 mg/L
According to OECD TG 202 the exposure concentration selection should be based on the EC50 (24 h) for immobilization, starting at about the 24 h EC50 and ending at about 1/100 of the 24 h EC50. The effects of the test concentrations were far lower (CITI, 1997).
Reference
Nominal/measured Concentration:
Measured concentration (mg/L) (percentage of nominal) |
|||||
Nominal concentration (mg/L) |
0-day |
7-day |
14-day |
21-day |
Mean* |
control |
n.d. |
n.d. |
n.d. |
n.d. |
- |
Solvent control |
n.d. |
n.d. |
n.d. |
n.d. |
- |
0.128 |
0.103 (80.1) |
0.0854 (66.7) |
0.0878 (68.6) |
0.0860 (67.2) |
0.0904 (70.7) |
0.320 |
0.273 (85.4) |
0.230 (71.9) |
0.231 (66.5) |
0.200 (62.5) |
0.229 (71.5) |
0.800 |
0.703 (87.9) |
0.622 (77.7) |
0.785 (98.1) |
0.454 (56.8) |
0.641 (80.1) |
2.00 |
1.62 (80.8) |
1.54 (76.8) |
1.51 (75.3) |
1.19 (59.3) |
1.46 (73.0) |
5.00 |
4.08 (81.6) |
4.06 (81.2) |
3.67 (73.5) |
3.67 (73.5) |
3.87 (77.4) |
n.d. : <0.100 mg
*The values are express as arithmetic means calculated
Water Temperature: 19.9-20.3 degree C
Water Chemistry in test: pH 7.3-7.6; Disooved oxygen = 8.3-8.9 mg/L(Oxygen saturation level >=60%)
Effect Data
Mortality: 21 day LC50 > 3.87 mg/L
Reproduction: 21 day ErC50 = 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr = 1.46 mg/L; LOECr = 3.87 mg/L
Cumulative number of dead parental Daphnia:
Cumulative number of dead parental Daphnia Exposure time (day) |
|||||||||||||||||||||
Measured conc. (mg/L) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
control |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
1 (2.5) |
2 (5.0) |
4 (10.0) |
5 (12.5) |
6 (15.0) |
Solvent control |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
1 (2.5) |
2 (5.0) |
5 (12.5) |
7 (17.5) |
7 (17.5) |
0.0904 |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
1 (2.5) |
1 (2.5) |
1 (2.5) |
2 (5.0) |
2 (5.0) |
4 (10.0) |
0.229 |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
1 (2.5) |
3 (7.5) |
3 (7.5) |
8 (20.0) |
12 (30.0) |
13 (32.5) |
0.641 |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
1 (2.5) |
1 (2.5) |
2 (5.0) |
5 (12.5) |
1.46 |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
1 (2.5) |
1 (2.5) |
1 (2.5) |
1 (2.5) |
2 (5.0) |
3 (7.5) |
4 (10.0) |
5 (12.5) |
3.87 |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
0 (0) |
3 (7.5) |
3 (7.5) |
3 (7.5) |
3 (7.5) |
3 (7.5) |
3 (7.5) |
3 (7.5) |
4 (10.0) |
5 (12.5) |
The value in parentheses express mortality (%) of Daphnia
Time (days) required to first brood production during exposure
Vessel No. |
|||||
Measured concentration (mg/L) |
1 |
2 |
3 |
4 |
Mean* |
control |
7 |
7 |
7 |
7 |
7.0 |
0.0904 |
7 |
7 |
7 |
7 |
7.0 |
0.229 |
7 |
7 |
7 |
7 |
7.0 |
0.641 |
7 |
7 |
7 |
7 |
7.0 |
1.46 |
7 |
7 |
7 |
7 |
7.0 |
3.87 |
10 |
10 |
10 |
10 |
10.0 |
Mean cumulative number of Juvenile produced per adult during exposure:
Exposure time (day) |
||||||||||||
Measured conc. (mg/L) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
control |
0 |
0 |
0 |
0 |
0 |
0 |
4.4 |
4.4 |
4.4 |
38.0 |
38.0 |
42.6 |
Solvent control |
0 |
0 |
0 |
0 |
0 |
0 |
5.3 |
5.3 |
5.3 |
38.0 |
38.0 |
41.0 |
0.0904 |
0 |
0 |
0 |
0 |
0 |
0 |
9.4 |
9.4 |
9.4 |
45.7 |
45.7 |
52.0 |
0.229 |
0 |
0 |
0 |
0 |
0 |
0 |
7.4 |
7.4 |
7.4 |
40.7 |
40.7 |
47.7 |
0.641 |
0 |
0 |
0 |
0 |
0 |
0 |
7.6 |
7.6 |
7.6 |
46.9 |
46.9 |
53.3 |
1.46 |
0 |
0 |
0 |
0 |
0 |
0 |
5.8 |
5.8 |
5.8 |
34.3 |
34.3 |
40.1 |
3.87 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3.0 |
3.0 |
6.0 |
Exposure time (day) |
|||||||||
Measured conc. (mg/L) |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
20 |
21 |
control |
42.6 |
85.0 |
85.0 |
85.0 |
138 |
138 |
188 |
188 |
197 |
Solvent control |
41.0 |
76.4 |
76.4 |
76.4 |
129 |
129 |
171 |
171 |
185 |
0.0904 |
52.0 |
90.4 |
90.4 |
90.4 |
143 |
143 |
188 |
188 |
208 |
0.229 |
47.7 |
89.8 |
89.8 |
89.8 |
133 |
133 |
176 |
176 |
206 |
0.641 |
53.3 |
88.8 |
88.8 |
88.8 |
149 |
149 |
174 |
174 |
193 |
1.46 |
40.1 |
60.0 |
60.0 |
60.0 |
108 |
108 |
128 |
128 |
135 |
3.87 |
6.0 |
15.2 |
15.2 |
15.2 |
17.4 |
17.4 |
17.5 |
17.5 |
23.1 |
Description of key information
3,5,5-trimethylhexan-1-ol was tested in a Daphnia reproduction test according to OECD TG 202, Part. The NOECr was 1.46 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.46 mg/L
Additional information
3, 5,5 -trimethylhexan-1 -ol was tested in a Daphnia reproduction test according to OECD TG 202, Part 2, under GLP for 21 days at concentrations up to nominal 5 mg/L (measured 3.87 mg/L). The effect data are as follows:
Mortality: 21 day LC50> 3.87 mg/L
Reproduction: 21 day ErC50= 2.09 mg/L (95% c.i.: 1.94 - 2.25 mg/L); NOECr= 1.46 mg/L; LOECr= 3.87 mg/L
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