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EC number: 218-377-7 | CAS number: 2136-89-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- α,α,α,2-tetrachlorotoluene
- EC Number:
- 218-377-7
- EC Name:
- α,α,α,2-tetrachlorotoluene
- Cas Number:
- 2136-89-2
- Molecular formula:
- C7H4Cl4
- IUPAC Name:
- 1-chloro-2-(trichloromethyl)benzene
- Details on test material:
- Charge DEBR008539
Purity: 99.76% (GC-a/a), p-chlorobenzotrichloride < 0.1%
Constituent 1
- Specific details on test material used for the study:
- Purity: OCTC 99.66%, PCTC 0.11% unknown 0.23%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentrations of o-chlorobenzoic acid in all test solutions and test cultures will be
measured at the beginning of exposure and 72 hours after the beginning of exposure by high
performance liquid chromatography (HPLC). Samples taken from middle layer of vessels
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance is quickly hydrolyzed in water to produce o-chlorobenzoic acid as a
hydrolysate, and the half life of the test substance is less than 30 minutes (156.57 g of
o-chlorobenzoic acid is generated from 229.93 g of the test substance, calculated by the
testing facility). Therefore, the exposure test will be conducted after changing the test
substance completely to o-chlorobenzoic acid in test water.
The test concentration will be expressed by both the test substance and o-chlorobenzoic
acid. The concentration of o-chlorobenzoic acid will be measured in test solutions and test
culutures, and the effect concentration will be calculated based on the concentration of
o-chlorobenzoic acid. The obtained toxicity data will be converted into the concentration of
the test substance.
Stock volumes of 100mg/L are prepared using dilution water and stirred for 48 hours at 22C.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular green algae
- Strain: ATCC22662
- Source (laboratory, culture collection): ATCC
- Age of inoculum (at test initiation): 3-4 days
- Method of cultivation: period subculture in Gorham medium
ACCLIMATION
- Acclimation period: 3-4 days
- Culturing media and conditions (same as test or not): as test
- Any deformed or abnormal cells observed:
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22 +/- 2C
- pH:
- 8.0( 7.7 to 8.1 at 22 C)
- Nominal and measured concentrations:
- Nominal concentration of OCTC: 0, 10, 18, 32, 56, 100 mg/L
Nominal concentration of o-chlorobenzoic acid: 0, 6.8, 12, 22, 38, 68 mg/L
measured concentration of o-chlorobenzoic acid 0 hr: 6.56,11.8,20.4, 35.7, 63.4 mg/L
measured concentration of o-chlorobenzoic acid 72 hrs: 6.28, 11.3, 20.3, 34.7, 60.5 mg/L
Mean measured concentration of o-chlorobenzoic acid: 6.42, 11.5, 20.3, 35.2, 61.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass 300 ml fill volume
- Aeration: cultures shaken 100 rpm
- Initial cells density: 5x10^3 cells/mL
- Control end cells density:
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3 (three)
- No. of vessels per control (replicates): 6 (six)
- No. of vessels per vehicle control (replicates): 6 (six)
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 65-75 µE/m^2/s white fluorescent lamp
- Salinity (for marine algae):
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: base on results of rangefinding study
- Range finding study
- Test concentrations: 0.1, 1, 10 ,100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 16.9 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 of the test substance was found to be 35.1 mg/L and the NOEC was 16.9 mg/L
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