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EC number: 212-506-0 | CAS number: 822-89-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1994-05-20 to 1994-06-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-1 (Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydroxyl-2-pyridone
- EC Number:
- 212-506-0
- EC Name:
- Hydroxyl-2-pyridone
- Cas Number:
- 822-89-9
- Molecular formula:
- C5H5NO2
- IUPAC Name:
- 1-hydroxy-1,2-dihydropyridin-2-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (as cited in study reports): Oxy PYRION
- Physical state: solid (powder)
- Appearance: white speckled gold powder
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 93100020
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at ambient temperature in the original container until required.
- Stability under test conditions: not specified
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: 4 samples for possible analysis were taken from all test concentrations and the control at t=0 and t=96h. 20 mL of volume was taken from the approximate centre of the test vessels. On each occasion, two of the samples were analysed immediately and the other two were stored in a a refrigerator in case further analysis was required. Dilution water, either fresh or taken from the control vessels, served as blanks during analysis
- Sample storage conditions before analysis: two of the samples were analysed immediately and the other two were stored in a a refrigerator in case further analysis was required. The samples of aqueous test media (20 mL) were diluted with HPLC mobile phase and the concentrations of Oxypyrion in these solutions determined by high performance liquid chromatoraphy (HPLC) with a spectrophotometric detector
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium was prepared in duplicate. Each batch was prepared from a concentrated aqueous dispersion (2L) in which Oxypyrion (nominally 5 g) had been added to water; this was diluted in a test vessel to a final volume of fifty litres.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): All aqueous solutions were clear and colourless
Test organisms
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- Common name: Bluegill Sunfish
- Source: Aquatic Research Organisms, New Hampshire, USA. The fish were reared at Osage Catfisheries Inc., Missouri, USA.
- Length at study initiation (length definition, mean, range and SD): mean length 4.6 cm
- Weight at study initiation (mean and range, SD): mean weight 1.6 g
- Feeding during test: no. The fish were last fed ~ 20 hours before the start of the definitive test.
ACCLIMATION
- Acclimation period: at least 14 days after delivery in an aerated supply of dilution water under flow-through conditions
- Acclimation conditions (same as test or not): yes
- Monitored water quality during the 14 day holding period:
* Temperature: 21.1 - 21.4 °C
* pH values: 7.5 - 7.7
* DO (% ASV): 88-100%
* water hardness: 58 - 67 mg/L CaCO3
- Type and amount of food: pelleted fish food, supplemented with live Daphnia magna when available
- Feeding frequency: daily
- Health during acclimation (any mortality observed): mortality less than 1%, 14 days before test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 54-56 mg/L as CaCO3
- Test temperature:
- 20.9-22.2 °C
- pH:
- At t= 0h: 7.4-7.7
At t= 96h: 6.2-7.2 - Dissolved oxygen:
- 85-100 % Air Saturation Value
- Salinity:
- not relevant
- Nominal and measured concentrations:
- Final test
Nominal concentrations: 0 and 100 mg/L
Measured concentrations (mg/L)* at t=0h: n.d., 96.12
Measured concentrations (mg/L)* at t=96h: n.d., 99.50
* average of 2 replicates - Details on test conditions:
- TEST SYSTEM
- Test vessel: all-glass aquaria
- Material, size, headspace, fill volume: 80L, all-glass filled with 50 litres
- Aeration: gently aerated during the test
- No. of organisms per vessel: 15
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: treated tap water. Treatment involved blending tap water previously filtered through activated carbon to remove chlorine, with tap water, which has been softened and treated by reverse osmosis. In the holding and test areas this water was further blended with a supply of softened water treated by reverse osmosis to reduce hardness to 40-75 mg/L as CaCO3. This water was held in an intyermediate tank were it was equilibrated to the test temperature and gently aerated before being supplied to the holding and test areas.
- Metals: Cd < 0.005 mg/L; Cu = 0.05 - 0.1 mg/L; Pb < 0.05 mg/L: Mercury < 0.0001 mg/L; Ni < 0.1 mg/L; Tin = 0.003 - 0.1 mg/L; Zn = 0.03 - 0.07 mg/L
- Pesticides: < 0.1 µg/L
- Chlorine: 26-28 mg/L (as chloride)
- Ca/Mg ratio: 87-93 mg/L / 3.7-4.1 mg/L (26:1)
- Salinity: Na = 12-13 mg/L; K = 2.0-2.1 mg/L (6:1)
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment
- Photoperiod: 16h light daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Fish were considered to be dead when no reaction was observed after touching the caudal peduncle and visible breathing movements were absent. In addition, fish that were convulsing or showing other severe forms of distress not considered transient in nature and likely to become more severe before the exposure is terminated, will be sacrificed for humane reasons. These fish will be treated as having died in the test, based on OECD guideline on humane endpoint (ENV/JM/MONO/(2000)7).
TEST CONCENTRATIONS
- Range finding study: combined/range finding study was performed
- Test concentrations: 0.10, 1.0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 97.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations:
* rangefinding test: no effects were observed
* limit test: one fish died between 24 and 48 hrs, no other mortalities occured. From 48 hrs onwards, seven fish (23%) exhibited minor sublethal effects which comprised darkened pigmentation. One fish exhibited lethargy but thatwas not sustained.
- Mortality of control: none
- Other adverse effects control: none
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of Oxypyrion to Bluegill Sunfish was determined in a 96-hour static test according to the OECD guideline 203.
Under the conditions of the present study, the 96-h LC50 was greater than 97.8 mg/L based on mean measured concentrations (95% confidence interval not determined). The results of the test can be considered reliable without restriction.
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