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Diss Factsheets
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EC number: 204-310-9 | CAS number: 119-27-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- This study was published online on 05 Decemeber
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- other: US Environmental Protection Agency. 2002. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, 4th ed. EPA 821/R-02/013. Washington, DC.
- Deviations:
- not specified
- GLP compliance:
- not specified
- Remarks:
- Study conducted to a high scientific standard and findings presented in a comprehensive test report.
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source of test material: Holston Army Ammunition Plant
- Appearance: dry yellow powder
- Purity: >95% - Analytical monitoring:
- yes
- Details on sampling:
- Laboratory control samples and duplicates and matrix spike and duplicates were analyzed with test samples at a frequency of 5%.
- Details on test solutions:
- DNAN was ground to a fine powder by mortar and pestle. The desired DNAN mass and moderately hard reconstituted water volume were magnetically stirred (StableTemp Ceramic Stirring Hot Plate,700rpm;Cole-Parmer Instrument) at 22”C in the dark.
Nominally (mg/L): 25 12.5, 6.3, 3.1, 1.6, and 0.8 DNAN - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Pimephales promelas (< 24h old) were obtained from a commercial source (Aquatic Biosystems) and held to acclimate overnight while being provided a daily ration of Artemia nauplii.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 25”C
- Nominal and measured concentrations:
- nominal
- Details on test conditions:
- Chronic toxicity tests using P.promelas were conducted in accordance with the USEPA at 25°C. Bioassays consisted of 5 to 6 test concentrations (nominally (mg/L) 25, 12.5, 6.3, 3.1, 1.6, and 0.8 DNAN). The chronic toxicity methods involved daily, static water renewals (80% for P. promelas) using freshly prepared DNAN solution. In-water and out-water samples were collected daily and submitted to the US Army Institute of Public Health for determination of concentration by gas chromatography (described in Analytical method). Water-quality parameters were assessed daily on Inter- and intraspecies sensitivity to dinitroanisole both in- and out-water samples. The chronic P. promelas bioassay was conducted for 7d, and each exposure concentration consisted of 5 replicates (250-mL glass beakers containing 10 fish and 200mL test medium) and received a twice-daily feeding ration of Artemia nauplii to support growth. Survival, biomass, and growth (dry wt) were assessed. Surviving fish were collected, dried in a desiccator for 24h, and weighed on a microbalance to 0.001mg (AX26DR [Delta Range]; Mettler Toledo).
- Key result
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Survival
- Key result
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 11.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Survival
- Key result
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- >= 8.8 - <= 11.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- / Survival
- Key result
- Duration:
- 7 d
- Dose descriptor:
- other: MATC = maximum acceptable toxicant concentration
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Survival
- Key result
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 11.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Sublethal
- Key result
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 24.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Sublethal
- Key result
- Duration:
- 7 d
- Dose descriptor:
- other: MATC¼maximum acceptable toxicant concentration
- Effect conc.:
- 16.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Sublethal
- Details on results:
- Chemical stability and fate dinitroanisole was stable under static conditions for at least 48h in both the absence and the presence of test organisms. Analysis of the concentration and stability of DNAN in both filtered and unfiltered moderately hard reconstituted water over a 48-h period indicated that the magnetic stirring method fully dissolved the DNAN to a stable concentration. No significant differences in DNAN concentration were observed between preparation methods (unfiltered vs filtered) or over time.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 50% lethal concentration (LC50) values of DNAN ranged from 8.8 mg/L to 11.2 mg/L, whereas NOEC of DNAN was found to be 2.8 mg/L for the tested fish species.
- Executive summary:
A determination of the chronic toxicity to fish of DNAN was conducted (Kennedy, A.J et al, 2014). Tests were conducted in accordance with standard guidance (U. S. Environmental Protection Agency (USEPA), 2002, EPA-821-R-02-013.
Groups of Pimephales promelas were expose to nominal concentrations of 25, 12.5, 6.3, 3.1, 1.6, 0.8 mg/L of DNAN.
It was concluded that 7-days 50% lethal concentration (LC50) values of DNAN ranged from 8.8 mg/L to 11.2 mg/L, whereas NOEC of DNAN was found to be 2.8 mg/L for the tested fish species.
Reference
Description of key information
The 7-day toxicity (NOEC) of DNAN was found to be 2.8 mg/L for the tested fish species.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 2.8 mg/L
Additional information
A determination of the chronic toxicity to fish of DNAN was conducted (Kennedy, A.J et al, 2014). Tests were conducted in accordance with standard guidance (U. S. Environmental Protection Agency (USEPA), 2002, EPA-821-R-02-013.
Groups of Pimephales promelas were expose to nominal concentrations of 25, 12.5, 6.3, 3.1, 1.6, 0.8 mg/L of DNAN.
It was concluded that 7-days 50% lethal concentration (LC50) values of DNAN ranged from 8.8 mg/L to 11.2 mg/L, whereas NOEC of DNAN was found to be 2.8 mg/L for the tested fish species.
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