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EC number: 807-577-5 | CAS number: 1182844-21-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 10, 2012 - Dec 04, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- 1996
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken (at least in duplicate) from each concentration during the Limit Test at 0, 24, 48, 72 and 96 h. On each occasion, one sample was analysed after pre-treatments (see below); the remaining samples were retained in case further analysis would be required.
Pre-treatment method ofthe sample
50.0 ml collected water samples were diluted to 100 mL with 80% acetonitrile solution (acetonitrile: water = 80:20 (v:v)) and filtered with a 0.22 μm millipore membrane( discarding the initial 1 0mL filtrates ), then the filtrates were analysed by UPLC. - Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test item directly added into dilution water, ultrasonicated for 20 min. For the limit test WAF solutions with a nominal concentrations of 100 and 150 mg/L were prepared. Aqueous test substance mixture was stirred for 24 h at 23-25°C. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish (Batch F20120315)
- Source: Guangzhou Hongfa Aquatic Animals Culture Company, China
- Length at study initiation: mean 2.38 cm
- Weight at study initiation: mean 0.25 g
ACCLIMATION
- Acclimation period: Feeding fry held for 28 days supplied with a continuous flow of aerated water before test. Fish to be used for test was held for 7 days under test conditions.
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: crude protein >49.0%, crude fat >5.0%, crude fibre <3.0%, crude ash <12%, moisture <10.0% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 122.2 mg/L CaCO3
- Test temperature:
- 23.2 °C to 24.0 °C
- pH:
- 7.55 - 7.67
- Dissolved oxygen:
- 80-93 % of saturation
- Nominal and measured concentrations:
- Nominal concentration 100 mg/L WAF measured range: 0.116 - 0.125 mg/L
Nominal concentration 150 mg/L WAF measured range: 0.106 - 0.128 mg/L - Details on test conditions:
- TEST SYSTEM
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.7 - 0.8 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: 1000 - 1500 lux
EFFECT PARAMETERS MEASURED:
mortality, inactivity, abnormal swimming pattern, other abnormal behaviour recorded at 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10.0, 100 mg/L
- Full range
- Test concentrations: 100.0, 150 mg/L (WAF) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: accomodated fraction (WAF)
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.128 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: value based on measured concentration
- Results with reference substance (positive control):
- 24h-LC50: 269 mg/L (potassium dichromate)
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study no toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >150 mg/L (WAF) and > 0.128 mg/L (measured concentration).
- Executive summary:
Study design
The test item was tested in a GLP study according to OECD GL 203.A range-finding test and then a limit test were performed. Nominal concentrations of 1, 10 and 100 mg/L were used in the range-finding test, while nominal concentrations of 100 and 150 mg/L WAFs were used in the limit test. Water samples taken from the control and the test treatments in the limit test were analysed. The mean analyzed concentrations were 0.120 mg/L and 0.128 mg/L respectively. The analytical results showed that the concentration of the test item is consistent in the test medium throughout the 96-hour test period ( deviation within 20% ). Thus a static procedure was reasonable.
Results
In the range-finding test, 5 fishes per treatment and no replicates were used, while 10 fishes per treatment and 3 replicates in the limit test in the limit test. The test fishes were exposed for 96 hours to the test solution. During the whole test period, the pH values of the control media and test media were between 7.55 and 7.67, and the Dissolved Oxygen (DO) values varied from 80% to 93% of the air saturation at the test temperature, and the total hardness was in the range of 119 to 125 mg (CaCO3)/L.
During the test, the temperature ofthe test media were maintained in the range of 23.2°C to 24.0°C, at\d all fishes in the control group were n01mal. With the same conditions, K2Cr2O7was used as the positive control substance, and the resulting 24 h-LC50 was 284 mg/L. So the study met the acceptability criteria prescribed by the protocol (pH: 6.0~8.5; dissolved oxygen concentration: > 60% of the air saturation value; total hardness: 10~250 mg (CaCO3)/L; temperature: (23 ±2) °C; 24 h-LC50 of K2Cr2O7in the range of 200 to 400 mg/L). Therefore the test was considered valid.
During the whole test period, all fish in the control groups and treated groups were alive and appeared normal. The results showed that under valid static test conditions, the 96 h-LC50 of the test item to zebra fish is greater than the nominal concentration of 150 mg/L WAFs (measured concentration 0.128 mg/L), which the maximum concentration causing no m01iality of the test item to zebra fish is the nominal concentration of 150 mg/L WAFs (measured concentration 0.128 mg/L).
Conclusion
In this study no toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >150 mg/L (WAF) and > 0.128 mg/L (measured concentration).
Reference
Description of key information
The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 203. In this study no toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >150 mg/L (WAF) and > 0.128 mg/L (measured concentration).
Key value for chemical safety assessment
Additional information
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