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Diss Factsheets
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EC number: 916-899-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [Reaction mass of 2,7-Naphthalenedisulfonic acid, 5-amino-3-[[4-[2-[4-[(7-amino-1-hydroxy-3-sulfo-2-naphthalenyl)azo]-2-sulfophenyl]ethenyl]-3-sulfophenyl]azo]-4-hydroxy-, compd. with 2,2',2''-nitrilotris[ethanol] (1:5) and 3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:6) and 3,3'-[vinylenebis[(3-sulpho-p-phenylene)azo]]bis[6-amino-4-hydroxynaphthalene-2-sulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:4)]
- EC Number:
- 916-899-6
- Molecular formula:
- not available
- IUPAC Name:
- [Reaction mass of 2,7-Naphthalenedisulfonic acid, 5-amino-3-[[4-[2-[4-[(7-amino-1-hydroxy-3-sulfo-2-naphthalenyl)azo]-2-sulfophenyl]ethenyl]-3-sulfophenyl]azo]-4-hydroxy-, compd. with 2,2',2''-nitrilotris[ethanol] (1:5) and 3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:6) and 3,3'-[vinylenebis[(3-sulpho-p-phenylene)azo]]bis[6-amino-4-hydroxynaphthalene-2-sulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:4)]
- Test material form:
- liquid
Constituent 1
- Radiolabelling:
- no
Study design
- Analytical monitoring:
- yes
- Buffers:
- Citrate Buffer (0.05 M): pH 4
Phosphate Buffer (0.05 M): pH 7
Borate Buffer (0.05 M): pH 9 - Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: glass volumetric flasks
- Sterilisation method: filtration through pre-assembled sterile 500 mL filters (Nalgene).
- Lighting: performed under dark conditions
- Measures taken to avoid photolytic effects: dark conditions
- Measures to exclude oxygen: each sterilized buffer solution was purged in a gentle stream of nitrogen for approx. 20 min to deplete any dissolved oxygen.
- open system
- No indication of the test material adsorbing to the walls of the test apparatus
TEST MEDIUM
- Volume used/treatment 10 mL
- Kind and purity of water: Millipore
- Mobile phase: Solvent A: 0.05 M Ammonium acetate in Millipore water, Solvent B:Acetonitrile
OTHER TEST CONDITIONS
- Adjustment of pH: buffers used
- Number of replicates:
- 3 replicates at each pH level
- Positive controls:
- no
- Negative controls:
- yes
- Remarks:
- Blanks were tested
Results and discussion
- Preliminary study:
- The average recoveries obtained in Tier 1 testing were always in the range of 90-110 %. All main components of the substance were hydrolyzed less than the 10 %. This indicated a sufficient hydrolytic stability of three components of the test item different buffer solutions after 5 days of incubation at 50 °C.
- Transformation products:
- not measured
Total recovery of test substance (in %)open allclose all
- % Recovery:
- > 93.4 - < 103
- St. dev.:
- 9.3
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- > 93.9 - < 99.9
- St. dev.:
- 10.1
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- > 95.9 - < 97.1
- St. dev.:
- 8.9
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 5 d
Dissipation DT50 of parent compound
- Key result
- Remarks on result:
- hydrolytically stable based on preliminary test
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is hydrolytically stable.
- Executive summary:
No significant hydrolysis (≤ 10 %) was observed in the preliminary test at 50 °C for all tested pH values (4, 7, 9) of the test item. This indicates a half-life period of analytes at 25 °C of more than 1 year (t0.5 25°C > 1 year). Thus, the test item was considered to be hydrolytically stable and a further testing (Tier 2 experiments) (according to OECD guideline 111) is not required.
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