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EC number: 201-941-1 | CAS number: 89-80-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one by using Pseudomonas citronellolis DSM 50332 as an inoculum.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one- Common name: Menthone - Molecular formula: C10H18O- Molecular weight: 154.2512 g/mol- Smiles notation: C1([C@@H](CC[C@@H](C1)C)C(C)C)=O- InChl: 1S/C10H18O/c1-7(2)9-5-4-8(3)6-10(9)11/h7-9H,4-6H2,1-3H3- Substance type: Organic- Physical state: Liquid- Other: Test chemical was obtained from Fluka, Neu-Ulm, Germany.
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- other: Pseudomonas citronellolis DSM 50332 (Microorganisms)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Test inoculum Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany.
- Duration of test (contact time):
- 21 d
- Initial conc.:
- 308.5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- TEST CONDITIONS- Composition of medium: Anoxic media was used for the study. The medium contained (per liter of distilled water) 1 g of NaCl, 0.1 g of MgCl2.7H2O, 0.04 g of CaCl2, 0.5 g of KCl, 0.125 g of NH4Cl, 0.2 g of Na2SO4, 0.4 g of KH2PO4, 1.2 g of K2HPO4, and 0.85 g of NaNO3. After autoclaving, 2 ml of a non-chelated trace element mixture, 2 ml of a selenite-tungstate solution, vitamins, and 20 ml of a 1 M NaHCO3 solution were added, and the pH was adjusted to 7.0.- Additional substrate: 10 mM nitrate, 0.5 ml 2,2,4,4,6,8,8-heptamethylnonane (HMN), and 2 mM menthone (test chemical) was added as an additional substrate. - pH: 7.0- pH adjusted: YesTEST SYSTEM- Culturing apparatus: Glass tubes (21 ml) were used as a test vessel for the study. CONTROL AND BLANK SYSTEM- Other: Pasteurized samples and inoculated preparations without nitrate or test chemical were used as controls.
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 1.13
- Sampling time:
- 21 d
- Remarks on result:
- other: Other details not known
- Details on results:
- Test substance undergoes 1.13% degradation by Test mat. analysis parameter in 21 days.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The percentage degradation of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one was determined to be 1.13% degradation by Test mat. analysis parameter in 21 days. Thus, based on percentage degradation, (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one is considered to be not readily biodegradable in water.
- Executive summary:
Biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one by using Pseudomonas citronellolis DSM 50332 as an inoculum.Test inoculum Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany. Initial test substance conc. used for the study was 308.5 mg/l (2 mM). Anoxic media was used for the study.The medium contained (per liter of distilled water) 1 g of NaCl, 0.1 g of MgCl2.7H2O, 0.04 g of CaCl2, 0.5 g of KCl, 0.125 g of NH4Cl, 0.2 g of Na2SO4, 0.4 g of KH2PO4, 1.2 g of K2HPO4, and 0.85 g of NaNO3. After autoclaving, 2 ml of a non-chelated trace element mixture, 2 ml of a selenite-tungstate solution, vitamins, and 20 ml of a 1 M NaHCO3 solution were added, and the pH was adjusted to 7.0.Enrichment cultures were inoculated with activated sludge obtained from a local wastewater plant (Lintel, Osterholz-Scharmbeck, Germany). Enrichment cultures were maintained and test chemical consumption was measured in 21-ml glass tubes containing 15 ml of anoxic medium (10 mM nitrate, 0.5 ml2,2,4,4,6,8,8-heptamethylnonane (HMN), and 2 mM menthone (test chemical)). The overpressure due to gas formation was measured with a syringe, and 10 mM nitrate was added when the electron acceptor was depleted. The gas formed was analyzed by gas chromatography. Bacterial growth was observed daily and required between 3 - 22 days. Monoterpene contents were determined with a gas chromatograph equipped with flame ionization detectors and connected to a digital data-analyzing system. The amounts of monoterpene dissimilated were calculated from the differences in monoterpene contents between the pasteurized controls and the grown enrichment cultures. A model MAT ITS 40 ion trap system was used for the gas chromatography-mass spectrometry analysis.Microbial growth on test chemical menthone was observed within 10 days to 3 weeks. The disappearance of test chemical in mud-free enrichment cultures was quantified.The percentage degradation of test substance (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one was determined to be 1.13% degradation by Test mat. analysis parameter in 21 days. Thus, based on percentage degradation, (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one is considered to be not readily biodegradable in nature.
Reference
Microbial growth on test chemical menthone was observed within 10 days to 3 weeks. The disappearance of test chemical in mud-free enrichment cultures was quantified.
Table: Amount of test chemical consumed by nitrate reducing enrichment cultures.
Substrate | Amt. of monoterpene consumed (µmol) | Amt. of nitrate consumed (µmol) | Bacterial growth | Amt. of gas formed (ml) | No. of electrons released from complete substrate oxidation to CO2 per no. of electrons consumed by nitrate reduction (%) |
Menthone | 22.6 | 150 | +0.254 | ND | 167 |
Where,
ND = not determined
Description of key information
Biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one by usingPseudomonas citronellolis DSM 50332 as an inoculum (Jens Harder and Christina Probian, 1995).Test inoculum Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany. Initial test substance conc. used for the study was 308.5 mg/l (2 mM). Anoxic media was used for the study.The medium contained (per liter of distilled water) 1 g of NaCl, 0.1 g of MgCl2.7H2O, 0.04 g of CaCl2, 0.5 g of KCl, 0.125 g of NH4Cl, 0.2 g of Na2SO4, 0.4 g of KH2PO4, 1.2 g of K2HPO4, and 0.85 g of NaNO3. After autoclaving, 2 ml of a non-chelated trace element mixture, 2 ml of a selenite-tungstate solution, vitamins, and 20 ml of a 1 M NaHCO3 solution were added, and the pH was adjusted to 7.0.Enrichment cultures were inoculated with activated sludge obtained from a local wastewater plant (Lintel, Osterholz-Scharmbeck, Germany). Enrichment cultures were maintained and test chemical consumption was measured in 21-ml glass tubes containing 15 ml of anoxic medium (10 mM nitrate, 0.5 ml2,2,4,4,6,8,8-heptamethylnonane (HMN), and 2 mM menthone (test chemical)). The overpressure due to gas formation was measured with a syringe, and 10 mM nitrate was added when the electron acceptor was depleted. The gas formed was analyzed by gas chromatography. Bacterial growth was observed daily and required between 3 - 22 days. Monoterpene contents were determined with a gas chromatograph equipped with flame ionization detectors and connected to a digital data-analyzing system. The amounts of monoterpene dissimilated were calculated from the differences in monoterpene contents between the pasteurized controls and the grown enrichment cultures. A model MAT ITS 40 ion trap system was used for the gas chromatography-mass spectrometry analysis.Microbial growth on test chemical menthone was observed within 10 days to 3 weeks. The disappearance of test chemical in mud-free enrichment cultures was quantified.The percentage degradation of test substance (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one was determined to be 1.13% degradation by Test mat. analysis parameter in 21 days. Thus, based on percentage degradation, (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one is considered to be not readily biodegradable in nature.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Experimental study and predicted data for the target compound (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (CAS No. 89-80-5) and varioussupporting studiesfor its structurally similar read across substance were reviewed for the biodegradation end point which are summarized as below:
In an experimental key study from peer reviewed journal (1995), biodegradation experiment was conducted for 21 days for evaluating the percentage biodegradability of test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (CAS no. 89-80-5) by using Pseudomonas citronellolis DSM 50332 as an inoculum. Test inoculum Pseudomonas citronellolis DSM 50332 was obtained from the Deutsche Sammlung von Mikroorganismen, Braunschweig, Germany. Initial test substance conc. used for the study was 308.5 mg/l (2 mM). Anoxic media was used for the study.The medium contained (per liter of distilled water) 1 g of NaCl, 0.1 g of MgCl2.7H2O, 0.04 g of CaCl2, 0.5 g of KCl, 0.125 g of NH4Cl, 0.2 g of Na2SO4, 0.4 g of KH2PO4, 1.2 g of K2HPO4, and 0.85 g of NaNO3. After autoclaving, 2 ml of a non-chelated trace element mixture, 2 ml of a selenite-tungstate solution, vitamins, and 20 ml of a 1 M NaHCO3 solution were added, and the pH was adjusted to 7.0.Enrichment cultures were inoculated with activated sludge obtained from a local wastewater plant (Lintel, Osterholz-Scharmbeck, Germany). Enrichment cultures were maintained and test chemical consumption was measured in 21-ml glass tubes containing 15 ml of anoxic medium (10 mM nitrate, 0.5 ml2,2,4,4,6,8,8-heptamethylnonane (HMN), and 2 mM menthone (test chemical)). The overpressure due to gas formation was measured with a syringe, and 10 mM nitrate was added when the electron acceptor was depleted. The gas formed was analyzed by gas chromatography. Bacterial growth was observed daily and required between 3 - 22 days. Monoterpene contents were determined with a gas chromatograph equipped with flame ionization detectors and connected to a digital data-analyzing system. The amounts of monoterpene dissimilated were calculated from the differences in monoterpene contents between the pasteurized controls and the grown enrichment cultures. A model MAT ITS 40 ion trap system was used for the gas chromatography-mass spectrometry analysis. Microbial growth on test chemical menthone was observed within 10 days to 3 weeks. The disappearance of test chemical in mud-free enrichment cultures was quantified. The percentage degradation of test substance (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one was determined to be 1.13% degradation by Test mat. analysis parameter in 21 days. Thus, based on percentage degradation, (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one is considered to be not readily biodegradable in nature.
In a prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound (2R,5S)-5 -methyl-2 -(propan-2 -yl)cyclohexan-1 -one (CAS No. 89 -80 -5) in the presence of mixed populations of environmental microorganisms was estimated.The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one is expected to be not readily biodegradable.
Next study was experimental study done from from authoritative database (HSDB, 2017), in this study the biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical. Activated sludge was used as a test inoculum for the study. The percentage degradation of test chemical was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
In a supporting study from authoritative database (J-CHECK and HSDB, 2017) biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 0% and 5% by BOD, TOC removal and GC parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
On the basis of above results for target chemical (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one (from peer reviewed journal and EPI suite, 2017) and for its read across substance (from authoritative database J-CHECK and HSDB, 2017), it can be concluded that the test substance (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one can be expected to be not readily biodegradable in nature.
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