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EC number: 805-172-8 | CAS number: 65646-25-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 April 2016 - 06 July 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- Gas chromatograph
- Details on sampling:
- The test samples were thawed with the aid of sonication. The test samples (100 mL) plus 30 g of sodium chloride were extracted directly with 100 mL of hexane in the same bottle. An aliquot of the hexane layer was taken for analysis.
- Vehicle:
- not specified
- Details on test solutions:
- Based on the results of the range-finding test the following test concentrations were assigned to the definitive test: 1.0, 3.2, 10, 32 and 100% v/v saturated solution.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- freshwater unicellular alga, representative of primary producers found in natural waters and can therefore be considered as an important non-target organism in freshwater ecosystems
- Test type:
- static
- Water media type:
- other:
- Remarks:
- The culture medium was prepared using reverse osmosis purified deionized water and the pH adjusted to 7.5 with 0.1N NaOH or HCl.
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 ± 1 °C
- pH:
- pH 7.9 at 0 hours to pH 8.2 at 72 hours
- Nominal and measured concentrations:
- 0 hour : Control; 1.0; 3.2; 10; 32; 100
72 hour : Control; 1.0; 3.2; 10; 32; 100 - Details on test conditions:
- Pre-culture conditions gave an algal suspension in log phase growth characterized by a cell density of 8.96 x 10E5 cells per mL. Inoculation of 900 mL of test medium with 5.0 mL of this algal suspension gave an initial nominal cell density of 5 x 10E3 cells per mL and had no significant dilution effect on the final test concentration. The flasks were plugged with polyurethane foam bungs and incubated (INFORS Multitron Version 2 incubator) at 24 ± 1 °C under continuous illumination (intensity approximately 7000 lux) provided by warm white lighting (380 – 730 nm) and constantly shaken at approximately 150 rpm for 72 hours.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- 95% confidence limits 13 - 19 mg/L
- Details on results:
- There were no statistically significant differences between the control, 0.87 and 2.8 mg/L test concentrations (P≥0.05), however all other test concentrations were significantly different (P<0.05) and, therefore the "No Observed Effect Concentration" (NOEC) based on growth rate was 2.8 mg/L. Correspondingly the "Lowest Observed Effect Concentration" (LOEC) based on growth rate was 8.5 mg/L.
- Results with reference substance (positive control):
- The results from the positive control with potassium dichromate were within the normal ranges for this reference item.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of Pseudokirchneriella subcapitata to the test item gave the following results based on the 0-Hour measured test concentrations:
Growth Rate: EC50 (mg/L)=16; 95% Confidence Limits (mg/L)=13-19; No Observed Effect Concentration (NOEC) (mg/L)=2.8; Lowest Observed Effect Concentration (LOEC) (mg/L)=8.5
Yield: EC50 (mg/L)=8.1; 95% Confidence Limits (mg/L)=7.8-8.3; No Observed Effect Concentration (NOEC) (mg/L)=2.8; Lowest Observed Effect Concentration (LOEC) (mg/L)=8.5
Reference
Description of key information
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results based on the 0-Hour measured test concentrations:
Growth Rate: EC50 (mg/L)=16; 95% Confidence Limits (mg/L)=13-19; No Observed Effect Concentration (NOEC) (mg/L)=2.8; Lowest Observed Effect Concentration (LOEC) (mg/L)=8.5
Yield: EC50 (mg/L)=8.1; 95% Confidence Limits (mg/L)=7.8-8.3; No Observed Effect Concentration (NOEC) (mg/L)=2.8; Lowest Observed Effect Concentration (LOEC) (mg/L)=8.5
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 16 mg/L
- EC10 or NOEC for freshwater algae:
- 2.8 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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