Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1975
- Reference Type:
- secondary source
- Title:
- Dinitrotoluene (isomers mixture), CAS No.: 25321-14-6
- Author:
- OECD SIDS
- Year:
- 2 004
- Bibliographic source:
- UNEP Publication
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
- Principles of method if other than guideline:
- The test was conducted in accordance with "Bioaccumulation test of chemical substance in fish and shellfish" stipulated in the Order Prescribing the Items of the Test Relating to the New Chemical Substance (1974, Order of the Prime Minister, the Minister of Health and Welfare, the Minister of International Trade and Industry No. 1). This guideline corresponds to "305C, Bioaccumulation: Degree of Bioconcentration in Fish" stipulate in the OECD Guidelines for Testing of Chemicals (1981)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- Reaction mass of 1-methyl-2,4-dinitrobenzene and 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,4-/2,6-DNT (technical mixture)
- Analytical purity: no data
Constituent 1
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Source: Sugishama fish farm
- Fish at start of incubation: ca. 30 g, ca. 10 cm
- After external desinfection under static conditions with 50 mg/l Terramycin and 7 g/l sodium chloride, the fish were reared in a flow through system for about 28 d
- Feeding during test: Fish feeding with pelleted food (Japan Haigo Shiryo K.K.), about 1 % of body weight twice per day
ACCLIMATION
- Fish were reared in an acclimatization tank (flow through system) for another 28 d at 25 +/- 2 °C
ion period:
Study design
- Route of exposure:
- aqueous
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 56 d
Test conditions
- Test temperature:
- 25°+/- 1°C
- Dissolved oxygen:
- 6-8 mg/L
- Details on test conditions:
- - Incubation of each 15-20 fish per level in glass tank containing 100 l of liquid each
- Water was groundwater from the Kurume Research Laboratories
- Water temperature, pH, dissolved oxygen were continuously measured
- Total hardness, COD, chloride, and other parameters were measured every 6 months - Nominal and measured concentrations:
- 0.025 mg/L
Results and discussion
Lipid content
- Lipid content:
- 6.8 %
- Time point:
- start of exposure
Bioaccumulation factor
- Key result
- Type:
- BCF
- Value:
- > 3.2 - < 21.2 dimensionless
Any other information on results incl. tables
With a concentration of 0.25 mg/L, a BCF of 0.6 - 2.9 was obtained.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.