Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-426-4 | CAS number: 95-51-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-08-24 to 1988-09-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-chloroaniline
- EC Number:
- 202-426-4
- EC Name:
- 2-chloroaniline
- Cas Number:
- 95-51-2
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 2-chloroaniline
- Details on test material:
- - Name of test material (as cited in study report): o-Chloraniline
- Physical state: yellow-orange, clear liquid
- Analytical purity: 99.6 % (IR spectroscopy)
- Impurities (identity and concentrations):
<0.01 % cyclohexylamine
<0.01 % N-isopropylaniline
<0.01 % 2-chloro-N-Isopropylanilin
0.08% aniline
<0.01% 2-Isopropylaniline
<0.01% o-nitrochlorobenzene
0.24% p-chloroaniline
<0.01% m-chloroaniline
<0.01% 2,4-dichloroaniline
<0.01% 2,5-dichloroaniline
<0.01% 2,3-dichloroaniline
<0.01% 2,3-dichloroaniline
<0.01% o-phenylendiamine
0.03 % unknown impurities
- Purity test date: 1 Feb 1988
- Lot/batch No.: Lagerkessel 15/ 594 488
- Stability under test conditions: stable throughout the test period
- Storage condition of test material: room temperature, dark
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen
- Age at study initiation: 2-3 months
- Weight at study initiation: 170-210 g
- Fasting period before study:
- Housing: 5 per macrolon III cage, changed every week, animals marked individually,
- Bedding: wood chips Type S8/15 Ssniff
- Diet : ad libitum; Altromin 1324
- Water: ad libitum; tap water
- Acclimation period: 5 d
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): 50
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12, artificial 14 W/m²
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- nose/head only
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: from Rhema Labortechnik, Hofheim, Germany
- Exposure chamber volume: 7 L
- Method of holding animals in test chamber: animals were held in place in special test tubes
- Source and rate of air: 10 L/ min; about 85 air changes /h
- Method of conditioning air: air was led through a bottle with prewarmed test substance
- System of generating particulates/aerosols: two-component jet and conditioned compressed air (480 kPa) were used to nebulize liquid test substance in the baffle
- Method of particle size determination: aerodynamic particle sizer with laser-velocimeter (TSI-APS 3300)
- Treatment of exhaust air: 70 % of the incoming air were withdrawn as exhaust air
- Temperature, humidity, pressure in air chamber:
aerosol: T= 20-22°C
Humidity=34 %
vapour: T= 20-22°C
Humidity= 32 %-35 % (group 2 and 3); 55 % (group 4)
TEST ATMOSPHERE (vapour)
- Brief description of analytical method used: Ratfisch RS 55 with FI detector
- Samples taken from breathing zone: yes
TEST ATMOSPHERE (aerosol)
- Brief description of analytical method used: not possible due to condensation
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: 37 to 44 % <5 µm
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.):
group 5: 5.53 µm/ 1.81
group 6: 6.11 µm/ 1.81 - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- analytical, vapour: 0.371, 0.745 or 1.156 mg/L, group 2,3 and 4, respectively
analytical, aerosol: 2.642 or 4.406 mg/L (maximal attainable dose), group 5 and 6, respectively - No. of animals per sex per dose:
- 5 per sex per dose
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observed twice a day (including weekends, not during actual exposure period), weighed before the test and on day 3, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- body weight: ANOVA [F-test:Box-test; if different Tukey-Kramer test with Howell modification]
necroscopy: Chi-Square test and pairwise Fisher's test
Results and discussion
- Preliminary study:
- no
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 4.406 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- no mortality observed
- Clinical signs:
- other: no signs of toxicity were observed in group 1 to 3 (up to 0.745 mg/L) group 4, 5 and 6 displayed following signs of toxicity: group 4 (11.556 mg/L, vapour): tremor (4 h-4 h) group 5 (26.421 mg/L, aerosol): reduced motor activity, tremor, face down positio
- Body weight:
- male rats of group 4, 5 and 6 and female rats of group 6 displayed delayed body weight gain on day 3
- Gross pathology:
- no pathophysiological changes were observed
Any other information on results incl. tables
Only slight symptoms of an irritating potential for respiratory organs (nasal secretion) at 4.4 mg/L (aerosol).
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category III
- Remarks:
- Migrated information T, R25 Criteria used for interpretation of results: EU
- Conclusions:
- Like other chloroanilines, the primary toxic effect of 2-chloroaniline is methaemoglobin
formation. Taking into account that humans are much more sensitive to methaemoglobin
producing substances than rats 2-chloroaniline is classified in Toxicity Category III. - Executive summary:
Märtins, T (1990)
2 -chloroaniline was tested for acute inhalation toxicity according to OECD 403 on male and female young adult wistar rats in a head/nose only apparatus for 4 h. The concentration in the test chamber was monitored throughout the experiment. Following concentration (analytical) of 2 -chloroaniline were applied as vapour: 0.371, 0.745 or 1.156 mg/L (group 2,3 and 4, respectively) and as aerosol up to the maximal attainable dose: 2.642 or 4.406 mg/L, group 5 and 6, respectively. Controls were exposed in the same way to a water/air mixture.No mortality was observed in all groups. No pathophysiological changes were evident. Signs of toxicity were only observed in Group 4 to 6 and included: tremor, face down position, reduced mortor activity and in the highest dose group serous secretion from the nose, indicating an irritating effect to the respiratory system. Therefore the LD50 value is greater than 4.406 mg/L. Based on this study o-Chloroaniline showed only slight toxic potential without mortality and should be classified as harmul if inhaled.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.