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EC number: 248-258-5 | CAS number: 27138-31-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 2018 to May 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Oxydipropyl dibenzoate
- EC Number:
- 248-258-5
- EC Name:
- Oxydipropyl dibenzoate
- Cas Number:
- 27138-31-4
- Molecular formula:
- C20H22O5
- IUPAC Name:
- oxydipropyl dibenzoate
- Details on test material:
- - Name of test material (as cited in study report): Dipropylene glycol dibenzoate (DPGDB)
- Physical state: Clear colourless liquid
- Analytical purity: Not stated
- Lot/batch No.: 9903-28-1
- Expiration date of the lot/batch: 29 July 2000
- Storage condition of test material: Room temperature.
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Chemical marketing Concept, New Milford, Connecticut V988816101
- Expiration date of the lot/batch: 31 October 2019
- Purity test date:
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)
OTHER SPECIFICS:
- measurement of pH, osmolality, and precipitate in the culture medium to which the test chemical is added:
- other information:
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Days 0, 7, 14 adn 20 (new) - each treatment level and the control prior to division into replicate vessels
Dsya 1, 8, 15 and 21 (aged) - composite of all available replicate vessels within each treatment level and the control
The number of samples taken from each exposure solution was one at each interval.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was added directly to full volume of dilution water and was observed to be clear and colorless with oily globules of undissolved test substance on the surface. The solution was mixed slowly overnight using a Teflon-covered stir bar and magnetic stir plate. Following mixing overnight, the solution was observed to be clear and colorless with oily globules of undissolved test substance on the surface. The solubilized portion of the solution was carefully drained into a glass beaker from a spout on the bottom of the bottle, avoiding the surface of the solution. The resulting 100% of a 100 mg/L WAF solution was observed to be clear and colorless with no visible undissolved test substance following preparation. Based on stability information obtained during the trial, which determined that the stock was stable for up to 24 hours, the primary stock was prepared daily in the same manner as described above.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone:
- Justification for species other than prescribed by test guideline:
- Age at study initiation (mean and range, SD): <24 hrs
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Stage and instar at study initiation:
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Source: Smithers Viscient
- Age of parental stock (mean and range, SD):
- Feeding during test none
- Food type:
- Amount:
- Frequency:
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Remarks on exposure duration:
- 21-day reproduction study
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 170 to 180 CaCO3 (mg/L)
- Test temperature:
- 19 to 21 °C
- pH:
- 7.5 to 8.3
- Dissolved oxygen:
- 7.4 to 10
- Salinity:
- NA
- Conductivity:
- 740 to 790
- Nominal and measured concentrations:
- 0 (control), 2.6, 6.4, 16, 40 and 100 mg/L (as % of 100 mg/L WAF) nominal and control, 0.12, 0.33, 0.83, 2.2 and 5.6 mg/L geometric mean measured concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100-mL clear glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 80 mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): NA
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates):10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fortified laboratory well water
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study: 17-day preliminary range-finding exposure
- Test concentrations: control, 0.10, 1.0, 10 and 100 as % of 100 mg/L WAF
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: length
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: yes
- Other biological observations:
- Mortality of control: 10%
- Other adverse effects control:
- Immobilisation of control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations and the most sensitive endpoints, reproduction (total living offspring per surviving female) and length, the 21-day No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) were determined to be 40 and 100% of 100 mg/L WAF, respectively (2.2 and 5.6 mg/L as geometric mean measured concentrations, respectively).
- Executive summary:
The objective of this study was to evaluate the chronic effects of Benzoflex 9-88 on the survival, reproduction, and growth (mean total body length) of the daphnid, Daphnia magna. The exposure was performed under static-renewal conditions for a period of 21 days. Results of this study were used to determine the No-Observed-Effect Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC) for the most sensitive biological parameter monitored (e.g., daphnid survival, reproduction, and total body length). In addition, a median effective concentration (EC50), EC20, and EC10 values for survival, reproduction, and growth (total body length) were determined. The results of this study are based on nominal and geometric mean measured concentrations of Benzoflex 9-88.
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