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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
1984
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Green alga
- Source: National Institute for Environmental Studies, Tsukuba, Japan

ACCLIMATION
- Acclimation period: at least one month in a laboratory of the University of Tokushima
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
24 °C
Nominal and measured concentrations:
At least five different concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel: 100-mL Erlenmeyer flasks

GROWTH MEDIUM
- Standard medium used: yes; AAP medium according to Japan Society of Environmental Toxicity (2003): Handbook for testing ecological effects of chemical compound

OTHER TEST CONDITIONS
- Light intensity and quality: 5000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): number of algae every 24 hours
- Determination of cell concentrations: UV/Vis spectrophotometer; 450 nm
Reference substance (positive control):
yes
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
9.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Reported statistics and error estimates:
EC50 was calculated by linear correlation using a log-normalized plot.
Validity criteria fulfilled:
not specified

Description of key information

ErC50 (96 h) = 9.5 mg/L for Pseudokirchneriella subcapitata (OECD 201, full test)

NOErC (96 h) = 0.8 mg/L for Pseudokirchneriella subcapitata (OECD 201, full test)

Key value for chemical safety assessment

EC50 for freshwater algae:
9.5 mg/L
EC10 or NOEC for freshwater algae:
0.8 mg/L

Additional information

One publication is available by Yamamoto et al. (2007a) on the short-term toxicity towards aquatic algae. The study was conducted according to OECD 201 guideline but not reported in as much detail as current reports according to GLP standards. Pseudokirchneriella subcapitata were exposed for 96 h in a full test. An ErC50 (96 h) of 9.5 mg/L and a NOErC (96 h) of 0.8 mg/L were determined.