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Diss Factsheets
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EC number: 209-968-0 | CAS number: 599-64-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed per OECD Test Guideline 203 following GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-(α,α-dimethylbenzyl)phenol
- EC Number:
- 209-968-0
- EC Name:
- 4-(α,α-dimethylbenzyl)phenol
- Cas Number:
- 599-64-4
- Molecular formula:
- C15H16O
- IUPAC Name:
- 4-(2-phenylpropan-2-yl)phenol
- Reference substance name:
- 290-968-0
- IUPAC Name:
- 290-968-0
- Details on test material:
- p-Cumylphenol (PCP, CAS #599-64-4); Purity: 98-100% received as a white crystalline solid.
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples (50 mL) for chemical analyses were collected from the control and all test concentrations used in the test at the beginning and end of the 96-hour test period. If there was 100% mortality in the test concentration before 96 hours, then a sample was collected on the day of the observed mortality. All samples were frozen on dry ice in sealed 50 mL polypropylene centrifuge tubes before shipping to Reimer Analytical Associates, Inc. (Vancouver, B.C., Canada) for analysis.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- The test organism was rainbow trout (Oncorhynchus mykiss). The test was conducted with larval fish with fork lengths of 4.0 ± 1.0 cm and wet weights ranging from 0.3 to 0.5 g (approximately 50 days post hatch). The fish were obtained from a certified, disease-free commercial supplier. They were held at 15 ± 2 degrees C under an 8 hour dark and 16 hour light photoperiod, with a light intensity at the water surface of 133 lux. The fish were held in dechlorinated City of Calgary tap water. Dechlorinated tap water was the dilution and control water in the test. The fish were held 20 days before the test. Mortality in the stock culture was less than 0.1% the week prior to test initiation. The fish were fed a daily ration of trout chow equal to 5% of their body weight. The fish were not fed for 24 hours prior to test initiation or during the test.
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 15 ± 2 degrees C
- Dissolved oxygen:
- 72 to 100% of saturation
- Nominal and measured concentrations:
- Nominal concentrations: 0.2, 0.3, 0.6, 1.3, 2.5, 5.0, and 10.0 mg/L
Measured concentrations: < 0.2, < 0.2, 0.2, 0.5, 1.5, 2.2, and 4.2 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- >= 1.5 - <= 4.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
The measured initial concentrations of p-Cumylphenol were, on average, 64% lower than nominal concentrations. Final measured concentrations of p-Cumylphenol were, on average, 44% lower than nominal concentrations. All final measured concentrations were higher than initial measured concentrations. The coefficient of variance on duplicate analyses of p-Cumylphenol from low, medium, and high test concentrations was 10%. Values ranged from 3 to 19% and there was no apparent trend with the concentration of p-Cumylphenol. The variance at low concentrations was of the same relative magnitude as the higher concentrations. Although this variability may account for some of the differences, the measured concentrations were consistently lower than nominal concentrations and the differences were greater than 10 %. The toxicity values were derived with an average of the initial and final measured concentrations because the differences between the measured and nominal values were greater than 20 %.
There was 100% mortality in the 1.5, 2.2, and 4.2 mg/L test concentrations after 24 hours of exposure. The fish exposed to 0.5 mg p-Cumylphenol per L appeared stressed (lying on side and back flushing gills). The fish exposed to lower test concentrations appeared normal. The maximum concentration of p-Cumylphenol that did not cause any mortality within the 96-h test period was 0.5 mg/L. The minimum concentration of p-Cumylphenol that caused 100% mortality within the test period was 1.5 mg/L. An LC50(concentration causing lethality to 50% of the organisms) was determined to be 0.9 mg/L at 24, 48, 72 and 96 hours.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this study, the LC50 for p-Cumylphenol exposure was determined to be 0.9 mg/L at 24, 48, 72 and 96 hours.
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