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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- None
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- - Deion. water instead of dest. water for the stock solution. - Preparation of the inoculum : higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines) - 4 instead of 5 test concentrations
- Principles of method if other than guideline:
- Guideline followed
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Code number: FAT 20042/D
- Batch no. : 276
- Purity: ca 50%
- Solubility: insoluble
- Storage: room temperature
- Expiration date: 10/98 - Analytical monitoring:
- no
- Details on sampling:
- None
- Vehicle:
- not specified
- Details on test solutions:
- 320, 100, 32 and 10 mg/l of the test substance
32, 10 and 3.2 mg/l of the reference substance - Test organisms (species):
- activated sludge
- Details on inoculum:
- Organism: Activated sludge of a communal sewage treatment plant (ARA-Birsig Therwil)
Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- None
- Hardness:
- None
- Test temperature:
- 21.0 °C
- pH:
- 7.0 before the test beginning, after 3 h : 7.5 - 7.9
- Dissolved oxygen:
- None
- Salinity:
- None
- Conductivity:
- None
- Nominal and measured concentrations:
- 320, 100, 32 and 10 mg/l of the test substance
32, 10 and 3.2 mg/l of the reference substance - Details on test conditions:
- Sludge dry weight: 1.44 g/L
Test volume: 100 mL
Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV : 00 109)
Aeration: 0.1 - 0.2 l/min by pasteur-pipette
pH of sludge: 7.0 before the test beginning - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- The % inhibitions observed with 320, 100, 32 and 10 mg/L test concentrations were 3.27, 1.96, 4.58 and 12.42 % respectively.
- Results with reference substance (positive control):
- IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 9.5 mg/l
- Reported statistics and error estimates:
- The respiration rate of each test concentration was calculated as a percentage of the mean of the two control respiration rates. The results were plotted on a logarithmic (concentration) / normal (% inhibition) graph, where the IC50 was evaluated.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 (3 h) of the test substance FAT 20042/D was determined to be >320.0 mg/L
- Executive summary:
The toxicity of FAT 20042/D to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. The % inhibitions observed with 320, 100, 32 and 10 mg/L test concentrations were 3.27, 1.96, 4.58 and 12.42 % respectively. Based on the findings, the IC50 (3 h) of the test substance FAT 20042/D was determined to be >320.0 mg/l and the IC50 (3 h) of the reference substance 3,5-Dichlorophenol was 9.5 mg/l.
Reference
None
Description of key information
The IC50 of Acid Red 195 is greater than 320 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
Additional information
The toxicity of FAT 20042/D to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l. The % inhibitions observed with 320, 100, 32 and 10 mg/L test concentrations were 3.27, 1.96, 4.58 and 12.42 % respectively. Based on the findings, the IC50 (3 h) of the test substance FAT 20042/D was determined to be >320.0 mg/l and the IC50 (3 h) of the reference substance 3,5-Dichlorophenol was 9.5 mg/l.
In other supporting studies that evaluated toxicity to microorganisms, the IC50 for Acid Red 195 was found to be >300 mg/l.
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