Registration Dossier
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EC number: 801-694-5 | CAS number: 1392325-86-8
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity to soil microorganisms was examined in a study in accordance with OECD TG 216, and in compliance with GLP criteria. In this study, soil microflora in soil enriched with lucerne meal was exposed to nominal test substance concentrations of 0 (control), 10, 32, 100, 320, and 1000 mg/kg soil dw for 28 days. The test item was dissolved in acetone and mixed with fine quartz sand. After evaporation of the acetone, the quartz sand was mixed into the soil. No analytical verification of nominal test concentrations was performed (or required). After the 28-day exposure period, the nitrate-N content compared to the control was determined, and the nitrate-N formation rate calculated. The validity criteria of the test were met.
At day 28, the differences of nitrate-N content compared to the control for the range of test concentrations were 3.48%, 13.97%, 20.24%, 81.35% and 34.52%, respectively. With respect to nitrate-N formation rate, differences to the control for the range of test concentrations were 10.97%, 32.73%, 53.19%, 195.42% and 84.78%, respectively. However, for both nitrate-N content and formation rate the differences were not statistically significant compared to the control (Student-test procedure, one-sided smaller, α = 0.05). Therefore, no ECx values could be calculated. The 28-d NOEC was determined to be >1000 mg/kg soil dw.
Key value for chemical safety assessment
Additional information
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