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EC number: 800-153-0 | CAS number: 1313206-64-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 23-05-1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, No certificate of Analyses included.
- Justification for type of information:
- The category and read-across approach are justified in both documents, please find this further information in the attached documentation part in the dedicated ESR for microorganisms toxicity "Supporting Read-Across document".
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A stock suspension was used of 1 g/L.
The test was performed using the following test concentrations: Control (duplicate), 6.3, 12.5, 25, 50 and 100 mg/L - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Secondary activated sludge was obtained from the WWTP Nieuwgraaf in Duiven. The WWTP Nieuwgraaf is an actvated sludge plant treating predominantly domestic waste water. The activated sludge concentration in the incubation vessels was 1.0 g DW/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Hardness:
- Sythetic sewage feed was used: This was made by dissolving 16 peptone, 11 g meat extract, 3 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O and 2.8 g K2HPO4 in one liter of deionized water.
- Test temperature:
- 21 °C
- pH:
- pH ranged from 6.7 to 7.3
- Nominal and measured concentrations:
- Control (duplicate), 6.3, 12.5, 25, 50 and 100 mg/L nominal
- Details on test conditions:
- The activated sludge respiration inhibition test was performed according to OECD test guideline 209. In this test the respiration rate of activated sludge fed with a standard amount of synthetic sewage was measured. The respiration rate of the same activated sludge in the presence of various concentrations of the test substance under otherwise identical conditions was also measured.
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 10.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 25.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% conf. lim. 24.6 - 25.7
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- 58.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The control respiration rates are within 0% of each other
- Reported statistics and error estimates:
- The respiration rate was expressed as a percentage of the mean of two controls of the respiration rate: (1 - 2Rs/(Rc1 + Rc2))*100 = percentage inhibition where: Rs = oxygen consumption rate at tested concentration of the test substance; Rc = oxygen consumption rate of controls.
The EC values were computed from the best fitted lines (least square method) through the points given by the probit of the percentage inhibition and the logarithm of the concentration of the test substance. Confidence limits were computed based on Fieller's Theorem. - Validity criteria fulfilled:
- yes
- Conclusions:
- Activated sludge EC50 (30 min) of Duomeen O is EC50 = 25.1 mg/L according to OECD TG 209.
- Executive summary:
In order to predict effects of chemicals in the environment and in biological waste water treatment plants, an activated sludge respiration inhibition test was performed. The toxicity to activated sludge was determined in accordance with OECD test guideline 209.
The toxicity of Duomeen O to activated sludge was determined by measuring the respiration ratee after a contact time of 30 minutes at various concentrations of the test substance. The inhibitory effect of Duomeen O at a perticular concentration was expressed as a percentage of the two controls. From the results ECvalues were calcualted. The EC50 of Duomeen O for activated sludge is 25.1 mg/L with 95% CL of 24.6 and 25.7 mg/L. The EC20 and EC80 are 10.7 and 58.7 mg/L resp.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP, limited details
- Justification for type of information:
- The category and read-across approach are justified in both documents, please find this further information in the attached documentation part in the dedicated ESR for microorganisms toxicity "Supporting Read-Across document".
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- other: 88/302/EWG (Teil C) EG amtsblatt L 133 (1988), S 118 - 122
- GLP compliance:
- no
- Vehicle:
- no
- Details on test solutions:
- Due to the low solubility of the test substance in water no stock solution was prepared. The definitive test concentrations were directly prepared in the test vessel by weighing. The test concentration range was 15.6 to 1000 mg/L.
A control without test substance or reference substance was included in the procedure. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Starting concentration: 1600 mg/L based on dry weight
Originating from: communal STP Frankfurt/M-Sindlingen
Pretreatment according to OECD guideline 209
No adaptation applied - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Test medium according to EG guideline
- Test temperature:
- 20 ± 2 ° C
- pH:
- 7.2 ± 0.2
- Salinity:
- Test medium according to EG guideline
- Nominal and measured concentrations:
- Nominal quantification is not required
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 18.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 234.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The control and reference tests results were within the required limits
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 of activated sludge for (Z)-N-9-octadecenylpropane-1,3-diamine is 66.0 mg/L
- Executive summary:
The EC50 (3 h) of activated sludge for (Z)-N-9-octadecenylpropane-1,3-diamine is 66.0 mg/L
Referenceopen allclose all
Respiration rates of the activated sludge, inhibition percentages, probit values and pH at various concentrations of test substance.
Concentration mg/L |
Activity mg O2/min.L |
Inhibition % |
Probit |
pH |
Control |
0.24 |
- |
6.7 |
|
Control |
0.24 |
- |
6.7 |
|
100.0 |
0.00 |
100 |
8.10 |
7.2 |
50.0 |
0.06 |
75 |
5.67 |
6.8 |
25.0 |
0.12 |
50 |
5.00 |
6.8 |
12.5 |
0.18 |
25 |
4.33 |
6.8 |
6.3 |
0.22 |
8 |
4.59 |
6.8 |
Description of key information
The 30min_EC50 of N-9-octa-decenyl-, (Z)- propane-1,3-diamine (CAS 7173 -62 -8) to microorganisms is 25.1 mg/L in an OECD TG 209 test. This data has been used as read-across for Amines, N-(C16-18 and C18-unsatd. alkyl)trimethylenedi-, diacetates (CAS 1313206 -64 -2). This is then converted to the corresponding value of the salt, resulting to an EC50 of about 34.2 mg/L (MW of C18-unsaturated diamine = 316, MW of diamine diacetate = 430).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 34.2 mg/L
Additional information
The lowest EC50 for microorganisms as observed in an OECD 209 test is 25.1 mg/L for N-9-octa-decenyl-, (Z)- propane-1,3-diamine (CAS 7173 -62 -8).
This value is used as a read-across for the N-C16-18-alkyl-(evennumbered) C18 unsaturated) propane-1,3-diamine (CAS 1313206 -64 -2). The substances have the same hydrophilic group and nearly the same hydrophobic group. The only difference concerns the unsaturation level which is higher for (Z)-N-9-octadecenylpropane-1,3-diamine (CAS 7173 -62 -8).
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