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EC number: 695-953-8 | CAS number: 204918-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Settled sludge used instead of a centrifuged sludge
- Qualifier:
- according to guideline
- Guideline:
- other: EEC L133 s. 118-122 (30 May 1988)
- Deviations:
- yes
- Remarks:
- Settled sludge used instead of a centrifuged sludge
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- prop-2-en-1-yl 2-(2-chloro-5-isocyanatobenzoyloxy)-2-methylpropanoate
- EC Number:
- 695-953-8
- Cas Number:
- 204918-22-9
- Molecular formula:
- C15H14ClNO5
- IUPAC Name:
- prop-2-en-1-yl 2-(2-chloro-5-isocyanatobenzoyloxy)-2-methylpropanoate
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on September 20, 2000.
The pH after collection was 7.6.
The sludge was separated from the aqueous layer by settling instead of centrifugation.
The pH of the sludge before use was 7.2.
The sludge concentration in the test bottles was 1.72G suspended solids/L.
Study design
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- The test with CA 2814 B was conducted at a temperature of 20 ± 1°C.
- pH:
- The pH of sludge was 7.6 after collection and 7.2 after preparation (before use).
The pH of the mixtures in the test bottles was 8.3 – 8.5 at the end of exposure time. - Nominal and measured concentrations:
- The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours.
The respiration rate of the same activated sludge was also measured in the presence of 1.5, 5.2, 23.5, 40.9 and 109.3 mg test item/L and 3.2, 10 and 23. mg/L reference item, respectively.
The sludge concentration in the test bottle was 1.72 g/L suspended solids/L. - Details on test conditions:
- Vessels: 250 ml BOD flasks with gas inlet
Water: Dechlorinated drinking water
Temperature: 20 ± 2°C
Duration: 3 hours
Stock solution: No stock solution prepared due to lack of solubility
Test item solutions: Calculated amounts of the test item to produce the desired test concentrations were mixed with 120 ml medium
(114 ml water and 6.4 ml nutrient solution) in 250 ml BOD flasks.
Then 80 ml of the sludge were added, mixed and aerated immediately.
Nutrient solution: 16 g Peptone; 11g Meat extract; 3.0 g Urea; 0.7 g NaCl; 0.4 g CaCl2.2H2O; 0.5 g MgSO4.7H2O; K2HPO4,
were dissolved and the volume made up to 1 litre with dechlorinated drinking water.
- Reference substance (positive control):
- yes
Results and discussion
- Details on results:
- The test item CA 2814 B was tested at nominal concentrations of 1.5, 5.2, 23.5, 40.9 and 109.3 mg test item/L after a contact time of 3 hours.
The test item was not homogeneously distributed in the test vessels.
At the end of exposure small drops were sticking on the surface of the test vessels.
The calculation of the following effect levels was based on nominal values.
EC50 (3h): > 100 mg/L
EC20 (3h): > 100 mg/L
EC80 (3h): > 100 mg/L - Results with reference substance (positive control):
- For the reference item (tested nominal concentrations: 3.2, 10 and 23. mg/L reference item)
EC50 (3h): 9.0 mg/L
EC20 (3h): 3.0 mg/L
EC80 (3h): 26.7 mg/L - Reported statistics and error estimates:
- The deviation of the respiration rates in controls was 4%.
The EC 50 (3h) of the reference item was 9.0 mg/L.
Since the deviation of the respiration rates of the controls was <15% and the EC 50 (3h) of the reference item was in the range of 5 to 30 mg/L, the test was valid in terms of the given validity criteria.
Any other information on results incl. tables
Oxygen consumption rate and inhibitory effects of the test item and reference
Sample |
Concentration (mg/L) |
Consumption (mg/L) |
Inhibition (%) |
pH |
Blank 1 Blank 2 |
0.0 0.0 |
41.4 39.7 |
-2 2 |
8.3 8.4 |
Reference 1 Reference 2 Reference 3 |
32.0 10.0 3.0 |
6.9 17.3 32.7 |
83 57 19 |
8.4 8.4 8.5 |
Test conc. 1 Test conc. 2 Test conc. 3 Test conc. 4 Test conc. 5 |
109.3 40.9 23.5 5.2 1.5 |
46.0 41.2 48.5 38.8 40.1 |
-14 -2 -20 5 1 |
8.5 8.4 8.5 8.4 8.5 |
The pH was measured after 3 h aeration.
Negative inhibition results, when obtained in a test, are a consequence of the comparison of oxygen consumption values obtained in the blank, and the higher oxygen consumption values obtained in the presence of the test item, which are due to biological variations between the various test samples.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (3h) for CA 2814 B is > 100 mg/L.
Based on the inherent toxicity of CA 2814 B in this test alone, the test item can be classified as :
“not toxic to bacteria”. - Executive summary:
Findings:
The test with CA 2814 B was conducted at a temperature of 20 ± 1°C.
The pH of sludge was 7.6 after collection and 7.2 after preparation (before use). The pH of the mixtures in the test bottles was 8.3 – 8.5 at the end of exposure time.
The EC50 (3h) of the reference item 3,5-Dichlorophenol was 9.0 mg/L.
The inhibitory effect of the test item was expressed as a percentage of the mean respiration rates of two controls. Following values were calculated based on nominal concentrations:
EC50 (3h): > 100 mg/L
EC20 (3h): > 100 mg/L
EC80 (3h): > 100 mg/L
Conclusion:
TheEC50 (3h) for CA 2814 B is > 100 mg/L.
Based on the inherent toxicity of CA 2814 B in this test alone, the test item can be classified as :
“not toxic to bacteria”.
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