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EC number: 800-038-5 | CAS number: 1071838-81-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Analysis of the test item was performed by the chemical analysis unit of INERIS (CARA/RESA) that is not included in the GLP areas of expertise of the Test Facility. This analytical phase has been inspected by the Quality Assurance Personnel of the Test Facility for both the technical phase and the raw data generated. Consequently, owing to the approach set up for the control of these data, we estimate that the analytical monitoring of the test item does not affect compliance with Good Laboratory Practices in this study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- CuGUN is rapidly hydrolyzed in test medium, in copper and N-guanylurea.
Then, the initial concentration and maintenance of the exposure concentrations during the test have been checked by analyzing hydrolysis products (copper and N-guanylurea).
Analyses have been performed on each test solutions at the beginning and the end of the test.
At T48h, for each test concentration and for the control, analysis has been conducted, on solutions containining daphnids ( by pooling the eight vessels for each test concentration) - Vehicle:
- no
- Details on test solutions:
- A stock solution of CuGUN at 10 mg/L was prepared in deionised water acidified (pH<4) with nitric acid. This stock solution has been diluted ten times to obtain a solution at 1 mg/L in dilution water. Appropriate quantities of this solution (1 mg/L) were introduced into the glass vessel containing dilution water, to reach the required concentrations.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus 1820
- Source: INERIS
- Age at study initiation (mean and range, SD): less than 24 hours
- Feeding during test: None - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- NONE
- Hardness:
- Dilution water (NF EN ISO 6341): 240 mg/L expressed as CaCO3
- Test temperature:
- 20°C+/- 1°C
- pH:
- pH at T 0h
Control : 7.5
Stock solution (10 mg/L): 4.0
Diluted solution (1 mg/L): 7.1 - Dissolved oxygen:
- Dissolved oxygen at T 0h
Control : 8.7 mg/L
Stock solution (10 mg/L): 7.7 mg O2/L;
Diluted solution (1 mg/L): 8.4 mg O2/L - Nominal and measured concentrations:
- Measurements à T 0h : see table 4
Measurements à T 48h : see table 5 - Details on test conditions:
- TEST SYSTEM
- Test vessel: disposable glass tubes of 20 mL, filled with 10 mL of test solution
- Type : open
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Composition of the dilution water (NF EN ISO 6341)
Chemicals Amount in 1 litre of dilution water
CaCl2, 2H2O 294.0 mg
MgSO4, 7H2O 123.3 mg
NaHCO3 64.75 mg
KCl 5.75 mg
All the chemicals were of recognised analytical grade purity.
- Alkalinity: Not measured
- Ca/Mg ratio: 4
- Conductivity: 647 µS/cm
- Dissolved oxygen concentration: 8.7 mg/L
- Culture medium different from test medium: Yes M4 Elendt
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: No
- Light intensity: Complete darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility at 24 and 48 hours
TEST CONCENTRATIONS
Range finding study
- Test concentrations: mg/L 1.00 - 0.33 - 0.11 - 0.037 - 0.004 (CuGUN initial nominal concentrations)
Definitive study
-Spacing factor for test concentration: 2
Test concentrations: mg/L 0.300 - 0.150 - 0.075 - 0.038 - 0.019 - 0.009 (CuGUN initial nominal concentrations) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.084 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: "CuGUN equivalent" concentration based on total copper measured concentration
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.072 - 0.097
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.132 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: "CuGUN equivalent" concentration based on measured N-guanylurea concentration
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.114 - 0.150
- Details on results:
- - Behavioural abnormalities: None
- Other biological observations: None
- Mortality of control: None
- Observation at 24h : Table 6
- Observation at 48h : Table 7
As CuGUN is hydrolizing in copper and N-guanyl urea, "CuGUN equivalent" concentrations have been calculated, based on :
-Total copper measured concentration (CUGUN equivalent = Total copper concentration *CuGUN molar mass /Copper molar mass)
-N-guanylurea concentration (CUGUN equivalent = N-guanylurea concentration *CuGUN molar mass/ 2*N-guanylurea molar mass)
with :
CuGUN molar mass = 391.75 g/mol
Copper molar mass = 63.5 g/mol
N-guanyl molar mass = 102 g/mol
See Table 10
Calculated values of "CuGUN equivalent" , based on hydrolysis product measurement were outside the range of 80 to 120% . Therefore, EC50 have been calculated using "CuGUN equivalent" concentration geometric mean (see table 11) - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Mobility 24h
- EC50: 1.23 mg/L (1.16 – 1.32 mg/L)
- Other: The EC50 24h is in the recommended range: 0.6–2.1 mg/L. This result confirms the appropriate sensitivity of the strain of Daphnia magna. - Validity criteria fulfilled:
- yes
- Remarks:
- In the control vessels, the immobilisation of the daphnids did not exceed 10% at the end of the test. The dissolved oxygen concentration at the end of the test was > 3 mg/L in control and test vessels.
- Conclusions:
- The test is valid, an EC50 (48h) of 0.084 mg/L was determined.
According to the Regulation EC 1272/2008, Copper Guanylurea nitrate as to be classified as Aquatic Acute 1. - Executive summary:
Young daphnids, aged less than 24 hours at the start of the test, have been exposed to the test item CuGUN at a range of concentrations between 0.009 mg/L and 0.300 mg/L for a period of 48 hours.
Immobilisation was recorded at 24 hours and 48 hours.
CuGUN was hydrolyzed, in dilution water, in copper and N-guanyl urea. Then, the exposure concentrations during the test have been checked by analyzing hydrolysis products (copper et N-guanyl urea) at T 0h and T 48h.
EC50 of immobilisation have been calculated using "CuGUN equivalent" concentrations
EC5048h
“CuGUN equivalent”concentrations
(Confidence interval at 95%)
based on total copper measured concentrations.
0.084 mg/L
(0.072 – 0.097)
based onN-guanyl urea measured concentrations.
0.132 mg/L
(0.114 – 0.150)
According to the Regulation EC 1272/2008, Copper Guanylurea nitrate as to be scored as Aquatic Acute 1.
Reference
Table 6 : Observation at T 24 Hours
Test concentrations (CuGUN initial nominal concentrations) mg/L |
Number of daphnids immobilised |
% of immobilisation |
||||||||
A |
B |
C |
D |
E |
F |
G |
H |
Total (/40) |
|
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.009 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.019 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.038 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.075 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.150 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.300 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
40 |
100 |
Table 7: Observation at T 48 Hours
Test concentrations (CuGUN initial nominal concentrations) mg/L |
Number of daphnids immobilised |
% of immobilisation |
||||||||
A |
B |
C |
D |
E |
F |
G |
H |
Total (/40) |
||
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.009 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.019 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.038 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.075 |
1 |
2 |
0 |
0 |
1 |
0 |
1 |
1 |
6 |
15 |
0.150 |
4 |
4 |
5 |
4 |
3 |
5 |
3 |
1 |
29 |
72.5 |
0.300 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
40 |
100 |
Table 8: pH at T 48h
Control |
Test item 0.038 mg/L |
Test item 0.300 mg/L |
7.7 |
7.7 |
7.7 |
Table 9: Dissolved oxygen at T 48h
Control |
Test item 0.038 mg/L |
Test item 0.300 mg/L |
8.3 |
8.4 |
8.1 |
Table 10 : “CuGUN equivalent” concentrations based on total copper measured concentration and onN-guanylurea measured concentration.
CuGUN initial nominal concentration (mg/L) |
T 0h |
T48h |
||
CuGUN equivalent concentration (mg/L) based on |
||||
Total copper |
N-guanyl urea |
Total copper |
N-guanyl urea |
|
0.009 |
0.005 |
<0.010 |
0.008 |
0.011 |
0.019 |
0.016 |
0.018 |
/ |
0.022 |
0.038 |
0.029 |
0.049 |
0.030 |
0.044 |
0.075 |
0.058 |
0.093 |
0.051 |
0.089 |
0.150 |
0.110 |
0.173 |
0.107 |
0.152 |
0.300 |
0.227 |
0.457 |
0.210 |
0.329 |
Table 11 : Geometric mean of “CuGUN equivalent” based on total copper and N-guanylurea measured concentrations
CuGUN initial nominal concentration (mg/L) |
Geometric mean of“CuGUN equivalent” |
|
based on Total copper |
based onN-guanyl urea |
|
0.009 |
0.006 |
/ |
0.019 |
/ |
0.020 |
0.038 |
0.029 |
0.046 |
0.075 |
0.054 |
0.091 |
0.150 |
0.108 |
0.162 |
0.300 |
0.218 |
0.388 |
Description of key information
Young daphnids, aged less than 24 hours at the start of the test, have been exposed to the test item CuGUN at a range of concentrations between 0.009 mg/L and 0.300 mg/L for a period of 48 hours.
Immobilisation was recorded at 24 hours and 48 hours.
CuGUN was hydrolyzed, in dilution water, in copper and N-guanyl urea. Then, the exposure concentrations during the test have been checked by analyzing hydrolysis products (copper et N-guanyl urea) at T 0h and T 48h.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.084 mg/L
Additional information
CuGUN was hydrolyzed, in dilution water, in copper andN-guanylurea. Therefore, EC5048h have been calculated based on measured concentrations of copper andN-guanylurea.
|
EC5048h “CuGUN equivalent” concentration (Confidence interval at 95%) |
Based on total copper measured concentrations. |
0.084 mg/L (0.072 – 0.097) |
Based onN-guanyl urea measured concentrations. |
0.132 mg/L (0.114 – 0.150) |
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