Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-908-3 | CAS number: 19444-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- prop-2-en-1-yl 2-hydroxy-2-methylpropanoate
- EC Number:
- 700-908-3
- Cas Number:
- 19444-21-4
- Molecular formula:
- C7H12O3
- IUPAC Name:
- prop-2-en-1-yl 2-hydroxy-2-methylpropanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: 2 x 50 ml of each concentration
- Sample storage conditions before analysis: Room Temperature
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Calculated amounts of the stock solution to produce the desired test concentrations and calculated amoutns of DMF to achieve identical vehicle concentrations were given into the reconstituted water and were homogeneously distributed.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Clone 5
- Source: Derived from culture from Solvias AG Ecotox Center.
- Age at study initiation (mean and range, SD): <24 hours
- Size at study initiation (sieved through a 1220µm sieve): <1220µm
- Feeding during test
- Frequency: No feeding during the 48 hour test period.
Study design
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- The water hardness of the freshly prepared test medium was determined at test initiation. The water hardness corresponded to 244 mg CaCO3/L.
- Test temperature:
- The water temperature was maintained constant throughout the duration of the test at 21 ± 1°C in all test concentrations.
- pH:
- The pH was 8.5 - 8.6 and 8.0 - 8.1 at the start and at the end of the exposure, respectively.
- Dissolved oxygen:
- The oxygen saturation was measured to be 101 - 102 % and 97 - 99 % at the start d at the end of the exposure, respectively.
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal test concentration were 6.25, 12.5, 25, 50 and 100 mg/L of CA 2215 A. The test item was homogeneously distributed in the test vessels at all test concentrations at the start of the test. The measured test concentrations of CA 2215 A were 6.2, 12.5, 24.1, 41.0 and 104.2 mg/L at the start of the expousure and 5.5, 11.5, 23.9, 41.2 and 104.5 mg test item/L at the end of exposure.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150ml glass beaker
- Material, size, headspace, fill volume: 100ml of test solution
- No. of organisms per vessel: 5 daphnids were exposed per test vessel.
- No. of vessels per concentration (replicates): 4 replicates for the test concentrations
- No. of vessels per control (replicates): 4 replicates for the control
- No. of vessels per vehicle control (replicates): 4 replicates per vehicle
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was used as test medium (M4-medium according to ELENDT)
- Intervals of water quality measurement: Start and end of test
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark with a 30 min. transistion period
- Light intensity: 1200 lux
EFFECT PARAMETERS MEASURED : Immobilization and other behavioural changes of the daphnids were recorded after 24 and 48 hours of exposure. Those animals not able to swim within 15 seconds after gentle agitation of the test container were considered to be immobile.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 951.6mg of CA 2215 A wee mixed and made up to 10 ml with DMF (ML1). 1.0 ml of ML1 was mixed and made up to 10.0 ml with DMF (ML2). 1.0 ml of mL2 was mixed and made up to 10 ml with DMF (ML3). These solutions were used to prepare test solutions with test concentrations of 0.1, 1.0 and 10 mg/L, respectively.
- Results used to determine the conditions for the definitive study: 1.6033 g of CA 2215 A were mixed and made up to 10 ml with DMF (stock solution 1). 5 ml of stock solution 1 were mixed and made up to 10.0 ml with DMF (stock solution 2). 5 ml of stock solution 2 were mixed and made up to 10 ml with DMF (stock solution 3). 5 ml of stock solution 3 were mixed and made up to 10 ml with DMF (stock solution 4). 5 ml of stock solution 4 were mixed and made up to 10 ml with DMF (stock solution 5). These stock solutions were used to prepare test solutions with test concentrations of 1.0, 2.0, 4.0, 8.0 and 16.0 mg/L, respectively.
The following nominal concentrations were selected for the definitive test: Final test concentrations were 6.25, 12.5, 25.0, 50.0 and 100.0 mg/L of CA 2215 A - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 21.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 24.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL
- Details on results:
- Other biological observations: At test concentrations of 6.25 mg/L no daphnids were immobiized after 24 hours and after 48 hours of exposure. Immobilization rates after 48 hours of exposure were 5, 75, 95 and 100% at test concentration of 12.5, 25, 50 and 100 mg?l , respectively.
- Mortality of control: No immobilization of daphnids was observed in the control (blank). In addition, during the biological phase of the study, no unexpected phenomena were recorded. Therefore, the validity criteria regarding the biology of the test were fulfilled.- Reported statistics and error estimates:
- Probit Analysis
Variable : dead Control: (no) At time: 24 h
EC_XX and 95% confidence limits
PERC. EC (PERC) LOWER UPPER
(mg/L) (mg/L) mg/L)
0.05 12.4602 7.5498 16.0508
0.10 14.5054 9.5426 18.1175
0.20 17.4365 12.5819 21.1301
0.50 24.7952 20.3304 29.7871
0.80 35.2595 29.3915 46.9329
0.90 42.3842 34.4791 61.5219
0.95 49.3412 39.0235 77.5518
Summary:
Goodness-of-fit Tests
Statistics Value DF Prob>Chi-Sq
Pearson Chi-Square 3.9735 3 0.2643
Variable DF Estimate Std Err ChiSquare Pr>Chi
SLOPE: 1 2.39039142 0.46381 26.56179 0.0001
Control Rate = 0.0000
Variable : dead Control: (no) At time: 48 h
EC_XX and 95% confidence limits
PERC. EC (PERC) LOWER UPPER
(mg/L) (mg/L) mg/L)
0.05 11.1172 6.9289 14.1549
0.10 12.8522 8.6549 15.9032
0.20 15.3195 11.2477 18.4460
0.50 21.4363 17.6889 25.7162
0.80 29.9953 25.0737 39.7769
0.90 35.7537 29.1983 51.4884
0.95 41.3336 32.8656 64.1948
Summary:
Goodness-of-fit Tests
Statistics Value DF Prob>Chi-Sq
Pearson Chi-Square 2.5795 3 0.4611
Variable DF Estimate Std Err ChiSquare Pr>Chi
SLOPE: 1 2.50514874 0.485211 26.65661 0.0001
Control Rate = 0.0000
Any other information on results incl. tables
Biological Observations
Exposure period (h) |
EC 50 (mg/L) |
95 % confidence limit (mg/L) |
Slope |
No effect level (observed) (mg/L) |
24 48 |
24.8 21.4 |
20.3 – 29.8 17.7 – 25.7 |
2.39 2.50 |
12.5 6.25 |
Immobilization of Daphnids after 24 and 48 hours of exposure
Initial number of daphnids per test concentration: 20
Concentration
nominal (mg/L) |
Number of daphnia immobilized after 24 h of exposure |
|||||
Replicate: |
|
|||||
1 |
2 |
3 |
4 |
Total |
% |
|
Blank Vehicle 6.25 12.5 25 50 100 |
0 0 0 0 3 4 5 |
0 0 0 0 4 4 5 |
0 0 0 0 3 5 5 |
0 0 0 0 3 5 5 |
0 0 0 0 13 18 20 |
0 0 0 0 65 90 100 |
Concentration
nominal (mg/L) |
Number of daphnia immobilized after 48 h of exposure |
|||||
Replicate: |
|
|||||
1 |
2 |
3 |
4 |
Total |
% |
|
Blank Vehicle 6.25 12.5 25 50 100 |
0 0 0 1 3 4 5 |
0 0 0 0 4 4 5 |
0 0 0 0 4 5 5 |
0 0 0 0 4 5 5 |
0 0 0 1 15 19 20 |
0 0 0 5 75 95 100 |
Measurement of Temperature, pH and Oxygen Saturation
Concentration nominal (mg/L) |
0 h* |
Final measurement 48 h |
||||
|
pH |
Oxygen (%) |
Temp (°C) |
pH |
Oxygen (%) |
Temp (°C) |
Blank Vehicle 6.25 12.5 25 50 100 |
8.6 8.6 8.6 8.6 8.6 8.6 8.6 |
102 101 101 101 101 101 102 |
21.9 |
8.0 8.1 8.0 8.0 8.0 8.0 8.0 |
99 98 99 98 97 98 98 |
21.7 |
*Measured in separate beakers
Analytically determined Concentrations of CA 2215 A
Labelling of specimens CA 2215 A |
Concentration of CA 2215 A (mg/L) |
% of nominal concentration |
|
Day 0 (0 h) |
Blank Vehicle 6.25 mg/l 12.5 mg/l 25 mg/l 50 mg/l 100 mg/l |
<0.04 <0.04 6.2 12.5 24.1 41.0 104.2 |
- - 99.2 100.0 96.4 82.0 104.2 |
|
|
|
|
Day 2 (48 h) |
Blank Vehicle 6.25 mg/l 12.5 mg/l 25 mg/l 50 mg/l 100 mg/l |
<0.04 <0.04 5.5 11.5 23.9 41.2 104.5 |
- - 88.0 92.0 95.6 82.4 104.5 |
Applicant's summary and conclusion
- Conclusions:
- The EC50 (48h) of CA 2215 A was determined to be 21.4mg/L based on nominal concentrations. The highest concentration with no observed effects was 6.25mg/L based on moninal concentrations.
- Executive summary:
Test concentrations of nominal 6.25, 12.5, 25.0, 50.0 and 100.0mg/L of CA 2215 A were selected based on results of a range-finding test and a preliminary test. Exposure started when the daphnids were placed in the test vessel containing the test solutions.
Twenty daphnids were exposed to each of the desired test concentrations under static conditions in M4-medium. For the test concentrations and the control, four replicates were performed. Each replicate consisted of five specimens in 100ml test solution. The daphnids were observed for immobilization and behavioural changes after 24 h and 48 h of exposure.
The water temperature was maintained constant throughout the duration of the test at 21±1 °C in all test concentrations. The oxygen saturation was measured to be 101 -102 % and 97 – 99 % at the start and at the end of exposure, respectively. The pH was 8.5 – 8.6 and 8.0 – 8.1 at the start and at the end of exposure, respectively.
The test item was homogeneously distributed in the test vessels at all test concentrations at the start of the test. The measured test concentrations of CA 2215 A were 6.2, 12.5, 24.12, 41.0, 104.2 mg/L at the start of exposure and 5.5, 11.5, 23.9, 41.2, 104.5 mg test item/L at the end of exposure.
The total content of CA 2215 A was found 99.2, 100.0, 96.4, 82.0 and 104.2 % of nominal concentration at test begin and 88.0, 92.0, 95.6, 82.4 and 104.5 % at test end.
Following values are based on nominal concentrations.
Exposure period
(h)
EC 50
(mg/L)
95 % confidence limit
(mg/L)
Slope
No effect level (observed)
(mg/L)
24
48
24.8
21.4
20.3 – 29.8
17.7 – 25.7
2.39
2.50
12.5
6.25
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.