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EC number: 604-102-1 | CAS number: 13893-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA 600/9-78-016/OTS/ASTM
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-amino-2,3-dimethylbutanenitrile
- Cas Number:
- 13893-53-3
- Molecular formula:
- C6H12N2
- IUPAC Name:
- 2-amino-2,3-dimethylbutanenitrile
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Test concentrations were prepared by preparing a stock solution in deionized water and serially diluting to obtain desired concentration.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Nominal and measured concentrations:
- - Based on the results of the range-finder, test concentrations (nominal) were set at 0, 0.01, 0.1, 0.5, 1.0, and 10 mg/L.
- Details on test conditions:
- - Temperature and light readings were measured throughout the test and were within acceptable limits.
- The static algal toxicity study on Selenastrum capricornutum was conducted in 250 mL Erlenmeyer flasks containing 100 mL of synthetic algal nutrient medium.
- The deionized water was filtered through a millipore Milli-Q water purification system.
- After the media was prepared, the pH was adjusted to 7.5 and filter-sterilized through a 0.45 um filter.
- To each flask was added 1 mL of algal inoculum containing 2 ×106 ± 10 % cells.
- The test vessels were incubated for 96 hours at 24 ± 2°C under continuous "cool white" fluorescent light and constant shaking.
- Temperature and light intensity were monitored throughout the study.
- Log phase growth was confirmed at 96-hours with a count of 6.9 x 105 cells/ml in the control.
- A 96-hour range finding study was conducted to determine the concentration range for the definitive study.
- Test flasks were prepared in triplicate for each test concentrations and the control.
- Gravimetric determinations of algal growth at each test concentration (0, 0.01, 0.1, 0.5, 1.0, and 10 mg/L) indicated percent effected as 7, 7, 7, 58, 95, and 100, respective to the concentrations tested.
- Statistical analysis of the concentration vs. effect data was obtained by employing a computerized LC50 program.
- This program calculated the LC50 statistic and its 95% C.L. using the moving average test.
- The method of calculation selected for use was that which gave the narrowest confidence limits for the LC50.
- The no effect level was determined by using ANOVA and a multiple means comparison test (Fisher’s LSD).
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.