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EC number: 457-830-5 | CAS number: 2557-13-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Qualifier:
- according to guideline
- Guideline:
- ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 457-830-5
- EC Name:
- -
- Cas Number:
- 2557-13-3
- Molecular formula:
- Hill formula: C9 H7 F3 O2
- IUPAC Name:
- Methyl, 3-Trifluoromethylbenzoate
- Reference substance name:
- 607-745-6
- EC Number:
- 607-745-6
- IUPAC Name:
- 607-745-6
- Details on test material:
- - Name of test material (as cited in study report): Methyl, 3-Trifluoromethyl Benzoate
- Substance type: mono costituent
- Physical state: liquid
- Analytical purity: 99.77 %
- Lot/batch No.: 250037
- Expiration date of the lot/batch: March 10, 2009
- Stability under test conditions: stable
- Storage condition of test material: room temperature in the dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): Different concentrations of the test item were mixed homogeneously into the soil which was filled in glass vessels before the earthworms were introduced on top of the soil
- Controls: deionised water
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Bred by IBACON in a breeding medium of cattle manure, peat, sand and straw, mainly fed with cattle manure, stored at room temperature.
- Age: ca. 11 to 12 months, with clitellium
- Weight : 300 to 600 mg
- Acclimatisation: 1 day, in artificial soil, under test conditions
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- Acclimatisation and exposure: 19°C to 21°C
- pH:
- pH range at experimental start: 5.6 to 6.0
pH range at experimental end: 5.6 to 5.9 - Details on test conditions:
- TEST SYSTEM
- Test container /Amount of soil :
Normal glass bottling jars (1 L), loosely covered by glass-lids to enable exchange of air and to minimise evaporation, filled with approximately 500 g (dry weight equivalent) artificial soil.
- replicates : 4 per treatment group
- individuals: 10 per test unit, i.e. 40 individuals per treatment group
SOURCE AND PROPERTIES OF SUBSTRATE (artificial soil):
Based on OECD 207 but with reduced organic matter content:
• 5.0% Sphagnum-peat, air-dried and finely ground (2 mm); (Floragard, Vertriebs GmbH für Gartenbau, 26138 Oldenburg, Germany)
• 20% Kaolin clay (Erbslöh, 65558 Lohrheim, Germany)
• approximately 0.2% chalk (CaCO3) added to adjust pH to 6.0 ± 0.5 (Merck, 64293 Darmstadt, Germany)
• approximately 74.8% fine quartz-sand (F34) containing more than 50% by mass of particle size 0.05 mm to 0.2 mm; (Quarzwerke Frechen, Postfach 1780, 50207 Frechen, Germany)
OTHER TEST CONDITIONS
- Light intensity: 400 to 790 lux
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.25 mg/kg soil dw
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 62.5 mg/kg soil dw
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 168.4 mg/kg soil dw
Any other information on results incl. tables
Effect of Methyl, 3-Trifluoromethyl Benzoate on Earthworm (Eisenia fetida) mortality and biomass (14 d)
Test Species |
Eisenia fetida |
|||||
Exposure |
artificial soil |
|||||
14 d NOEC |
31.25 mg/kg dry weight soil |
|||||
14 d LOEC |
62.5 mg/kg dry weight soil |
|||||
14 d LC50 |
168.4 mg/kg dry weight soil |
|||||
|
126.0 to 244.0 mg/kgdry weight soil |
|||||
Treatment Group |
Control |
Methyl, 3-Trifluoromethyl Benzoate |
||||
|
deionised water |
31.25 |
62.5 |
125 |
250 |
500 |
Mortality after 14 days [%] |
0 |
0 |
0 |
0 |
100 |
100 |
Significance (Fisher’s exact test,a= 0.05) |
- |
n.s. |
n.s. |
n.s. |
* |
* |
Body weight change after 14 days [%] |
-10.4 |
-13.5 |
-15.7 |
-18.5 |
- |
- |
Significance (Dunnett-test,a= 0.05) |
- |
n.s. |
* |
* |
- |
- |
- not applicable
n.s. = No statistically
significant differences compared to the control *
= statistically significantly different to the control
Applicant's summary and conclusion
- Conclusions:
- In a 14-day acute toxicity study with Methyl, 3-Trifluoromethyl Benzoate to earthworms (Eisenia fetida) the 14-day LC50 was determined to be 168.4 test item/kg soil (95% confidence limits: 126.0 mg/kg to 244.0 mg test item/kg soil.
The No Observed Effect Concentration (NOEC) related to mortality was determined to be 31.25 mg Methyl, 3-Trifluoromethyl Benzoate/kg soil. The Lowest Observed Effect Concentration (LOEC) was determined to be 62.5 mg Methyl, 3-Trifluoromethyl Benzoate/kg soil.
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