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EC number: 247-660-8 | CAS number: 26401-35-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Remarks:
- The time frame of 10 days is sufficient as DITA is readily biodegradable.
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Ditridecyl adipate is appropriate for read-across because it is considered as a similar substance, based on similar structure, toxicokinetic and toxicological and ecotoxicological profile useful for this interpolation (please see also read-across justification attached in chapter 13). - Reason / purpose for cross-reference:
- read-across: supporting information
- GLP compliance:
- yes
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: No earthworms died or showed other effects during the 14-day exposure period.
- Total mass of adults at beginning of test: Individual weight of 10 earthworms at test start was 420 ± 90 mg.
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: In the control the reduction in mean earthworm weight was < 20% (2.8%). A statistically (p < 0.05) significant reduction of 14% in mean earthworm weight was found at 1000 mg/kg soil dw, the highest concentration tested. However, this reduction was considered to be biologically not significant (< 20%). - Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Jun - 10 Jul 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- adoptd 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
- Version / remarks:
- adopted 2008
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil: The batch of the test item tested was a clear colourless liquid with a purity of > 99%. Since the test substance was insoluble in Milli-RO water (tap water purified by reverse osmosis), acetone (≥ 99.8%) was used as a solvent. Solubility in Milli-RO water and organic solvent was based on a pre-test performed at NOTOX B.V. Preparation started with a stock solution prepared at a loading rate of 4 g test substance per 10 mL acetone (stock A). No special treatment other than careful mixing was necessary to dissolve the test substance. Stocks B through E were subsequently diluted (0.5 mL of higher stock was made up to 5 mL with acetone) from stock A through D, respectively. The colour of the stocks ranged from clear and yellow in stock A, thought clear and slight yellow in stock B to clear and colourless in stocks C to E. Subsequently, 1.45 mL of stocks B to E were spiked on 10 g silver sand. For the highest concentration 5.24 mL of stock A was spiked on 40 g of sand and the solvent control received 5.24 mL of acetone on 40 g sand. Each sand - test substance mixture was thoroughly mixed and subsequently left for to allow the solvent to evaporate. Soil pH was set with 11.4 g CaCO3 per kg of artificial soil (Merck, Darmstadt, Germany). Spiked quartz sand was added to artificial soil (dry artificial soil, CaCO3and Milli-RO water mixed for 10 minutes) and the whole was mixed for another 10 min using a mixing device.
- Controls: solvent control with acetone
- Chemical name of vehicle: acetone
- Evaporation of vehicle before use: yes - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm (Eisenia fetida fetida)
- Source: Earthworms were bred at the NOTOX B.V. facilities.
- Age at test initiation: 4 months with clitellum
- Weight at test initiation: 420 ± 90 mg
BREEDING CONDITIONS
- Method of breeding: Each batch is started ith adult earthworms with well-developed clitella, by placing 100 of them into ca. 15 litres of culture medium in a plastic container. Adults were removed between day 14 and 28 and juveniles were allowed to hatch from the cocoons produced by the adults. Batches were split up and fresh culture medium was added in case of overcrowding. Worms were transferred to fresh medium regularly.
- Culture medium: Mixture of 40% sphagnum peat and 60% pot soil humidified with tap water. The pH of the mixture is 6.0 ± 1.0. When necessary, the medium was humidified with tap water during breeding.
- Feeding: Every 7 - 10 days with oat meal
ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): Mortality within 24 hours prior to the test was less than 10%. - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.5 - 6.6 at test start
7.4 at test end - Moisture:
- 35 - 37%
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1.8 L glass with lid
- Amount of soil or substrate: 500 g/vessel
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per vehicle control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Artificial soil as described in the guidelines: 10% sphagnum peat, 20% kaolin clay, 70% industrial sand M32; pH was adjusted by addition of calcium carbonate.
- Water content: 25 - 42% of the dry weight
OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 515-654 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mortality and clinical effects were observed on day 7 and 14 of exposure.
- Ten earthworms were weighed individually prior to the start of the test. For all vessels the cumulative weight of the ten earthworms per vessel was recorded. The latter was also done for all vessels at the end of the test.
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: solvent control, 0.1, 1.0, 10, 100 and 1000 mg/kg soil dw
- Results used to determine the conditions for the definitive study: No mortality or other effects occurred up to the highest test concentration. - Nominal and measured concentrations:
- Nominal: vehicle control and 1000 mg/kg soil dw
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: No earthworms died or showed other effects during the 14-day exposure period.
- Total mass of adults at beginning of test: Individual weight of 10 earthworms at test start was 420 ± 90 mg.
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: In the control the reduction in mean earthworm weight was < 20% (2.8%). A statistically (p < 0.05) significant reduction of 14% in mean earthworm weight was found at 1000 mg/kg soil dw, the highest concentration tested. However, this reduction was considered to be biologically not significant (< 20%). - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Test date: 05 Jan - 19 Jan 2012
- LC50 (14d): 75 mg/kg soil dw, 95% CL: 56 - 100 mg/kg soil dw - Reported statistics and error estimates:
- To determine the effect of the test item on body weight of Eisenia fetida fetida, control data were compared with the data of the 1000 mg/kg soil dw treatment with the Two sample t-test (TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman) after checking for normality (Chi-square) and homogeneity of variance (F-test).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is not harmful to soil macroorganisms.
Referenceopen allclose all
Description of key information
LC50/NOEC(14d) > 1000/ >= 1000 mg/kg (nominal) for mortality of Eisenia foetida (OECD 207, read across)
Key value for chemical safety assessment
Additional information
The time frame of 10 days is sufficient as DITA is readily biodegradable.
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