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EC number: 241-300-3 | CAS number: 17265-14-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
No data are available for the test article. However, closely related chemicals gave negative results when tested in an Ames test, chrmomsomal aberration study and in an HPRT study. In addition, no findings were reported in an in vivo micronuclues test. The test article is therefore considered to be not genotoxic.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- According to Ames et al., Mut. Res., 31, 1975
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source of test material: Tokyo Kasei Kogyo Co. LTD. Tokyo, Japan. - Target gene:
- S. typhimurium: his-
E. coli: trp- - Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- S. typhimurium TA 1538 / E.coli WP2 uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 1, 5, 10, 50, 100, 500, 1000, and 5000 µg/plate
- Vehicle / solvent:
- DMSO
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2 -(2 furyl)-3 -(5 -nitro-2 -furyl) acrylamide (AF2)
- Remarks:
- 0.01 µg/plate for TA100, 0.05 µg/plate for WP 2 uvrA and TA 98, without S9-mix
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- 5 µg/plate for TA1535 and for TA1535, without S9-mix
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- 80 µg/plate for TA1537, without S9-mix
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- 0.25 µg/plate for TA1538, without S9-mix
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- 5 µg/plate for TA1535 and WP2 uvrA, with S9-mix
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- 5 µg/plate for TA100, TA98, TA1537, TA1538, with S9-mix
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
- preincubation, added to a minimal glucose agar plate
DURATION
- Preincubation period: 20 min - Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Positive controls validity:
- valid
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- other: In Vitro Mammalian Cell Gene Mutation Test (HPRT)
- Target gene:
- HPRT
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 liver microsomal fraction from male Wistar rats
- Test concentrations with justification for top dose:
- 0.1, 0.25, 0.5, 1, 2.5, 5, 7.5, and 10 mM without metabolic activation
0.5, 0.75, 1, 2, 5, 4, 7, 8.5, and 10 mM with metabolic activation - Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- Key result
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- no metabolc activation was used
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Human embryonic lung fibroblast cultures (WI-38) were suspended in tissue culture medium and plated. The test compound was added at three dose levels using three bottles for each level, 24 hours after plating. A preliminary determination of tissue culture toxicity was performed (cytotoxic effects were observed at 400 mg/l). Cells were incubated at 37 degree Celsius and examined twice daily to determine when an adequate number of mitoses were present. Cells were harvested and fixed (3:1 absolute methanol : glacial acetic acid). The specimens were centrifuged, decanted, and suspended in acetic acid-orcein stain and dropped on a slide. The preparations were examined by microscopy. Cells in anaphase were observed for non-disjunction as indicative of cytogenetic damage. Analyzed aberrations include bridges, pseudochiasmata, multipolar cells, and acentric fragments. The positive control was triethylene melamine (TEM) and the negative control was saline. 100 cells were investigated per dose.
- GLP compliance:
- not specified
- Remarks:
- pre-GLP
- Type of assay:
- other: in vitro mammalian chromosome aberration test
- Species / strain / cell type:
- mammalian cell line, other: human fibroblasts (WI-38)
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 0, 2, 20, and 200 mg/L
- Untreated negative controls:
- yes
- Remarks:
- saline
- Positive controls:
- yes
- Positive control substance:
- triethylenemelamine
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: 24 h
STAIN (for cytogenetic assays): acetic acid-orcein
NUMBER OF METAPHASE SPREADS ANALYSED PER DOSE (if in vitro cytogenicity study in mammalian cells): 100 cells were investigated/dose.
DETERMINATION OF CYTOTOXICITY
- Any supplementary information relevant to cytotoxicity: Cytotoxic effects were observed at 400 mg/L in a preliminary tissue culture toxicity test.
- OTHER: Cells in anaphase were observed for non-disjunction as indicative of cytogenetic damage. Analyzed aberrations include bridges, pseudochiasmata, multipolar cells, and acentric fragments. - Key result
- Species / strain:
- mammalian cell line, other: human fibroblasts (WI-38)
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 400 mg/L, preliminary Cytotoxicity test
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Referenceopen allclose all
SUMMARY OF RESULTS
Compound | Dose (µg/plate) | TA100 | TA1535 | WP2 uvrA | TA98 | TA1537 | TA1538 | ||||||
-S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | ||
Revertant Colonies |
|||||||||||||
DMSO | 150 | 154 | 30 | 15 | 30 | 34 | 32 | 42 | 18 | 22 | 22 | 28 | |
AF-2 | 0.01 | 501 | |||||||||||
AF-2 | 0.05 | 1082 | 278 | ||||||||||
ENNG | 5.0 | 1101 | |||||||||||
9AC | 80.0 | 889 | |||||||||||
4NQO | 0.25 | 270 | |||||||||||
B(a)P | 5.0 | 1084 | 809 | 313 | 354 | ||||||||
2AA | 5.0 | 440 | 359 | ||||||||||
Sebacic Acid | 1 | 130 | 130 | 29 | 9 | 17 | 26 | 43 | 49 | 17 | 23 | 23 | 38 |
5 | 119 | 138 | 34 | 11 | 24 | 22 | 52 | 50 | 13 | 25 | 26 | 38 | |
10 | 131 | 130 | 26 | 15 | 23 | 27 | 39 | 46 | 14 | 24 | 31 | 33 | |
50 | 125 | 123 | 34 | 13 | 18 | 24 | 47 | 56 | 11 | 24 | 27 | 33 | |
100 | 136 | 119 | 36 | 16 | 26 | 29 | 50 | 47 | 13 | 29 | 36 | 39 | |
500 | 140 | 122 | 28 | 13 | 24 | 32 | 42 | 56 | 11 | 18 | 20 | 34 | |
1000 | 127 | 132 | 26 | 12 | 27 | 23 | 34 | 45 | 18 | 12 | 28 | 33 | |
5000 | 112 | 134 | 36 | 14 | 24 | 27 | 45 | 47 | 12 | 20 | 30 | 32 |
Negative and positive controls were functional. The negative controls contained two cells with bridges one of which contained an acentric fragment. The test compound was negative except for one cell which contained a bridge at the high dose level. In summary, the compound produced no significant aberration.
Dose level | Mitotic index** | Number of cells | % cells with acentric fragments | % cells with bridges | % multipolar cells | % cells other aberrations* | % cells with aberrations++ |
2 | 1 | 100 | 0 | 0 | 0 | 0 | 0 |
20 | 1 | 100 | 0 | 0 | 0 | 0 | 0 |
200 | 1 | 100 | 0 | 1 | 0 | 0 | 1 |
negative control | 1 | 100 | 1 | 2 | 0 | 0 | 3 |
positive control | 1 | 100 | 3 | 12 | 0 | 0 | 15 |
*Cells that have polyploidy (P), pulverization (pp), fragments (f) or greater than 10 aberrations (a)
** Percent of cells in mitosis : 200 cells observed/dose level
++ Duplicate aberrations in a single cell will cause this to be a % less than a summation of the % aberration seen
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
In a mammalian bone marrow chromosome aberration test similar to OECD Guideline 475 in rats (Litton, 1974), adipic acid was administered a single dose of 5000 mg/kg bw. Adequate positive and negative controls were valid. The mitotic indices were considered to be within the normal limits of the controls and there was no evidence for chromosomal damage.
Link to relevant study records
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Groups of 5 treated and 3 control animals were used. Animals were killed 6, 24 and 48 hours after a single administration in the acute study. In the subacute study 5 doses, 24 hours apart, were administered and animals were killed 6 hours after the last dose. Four hours after the last compound administration, and two hours prior to killing, each animal was given 4 mg/kg bw of colcemid intraperitoneally in order to arrest the bone marrow cells in mitosis. The marrow "plug" was removed and aspirated into Hanks' balanced salt solution. The specimen were centrifuged and resuspended in hypotonic 0.5% KCl. The specimens were placed in a 37 degree Celsius water bath in order to swell the cells. Following centrifugation the cells were resuspended in a fixative (3:1 absolute methanol : glacial acetic acid) and again centrifuged. Cells were resuspended and placed at 4 degree Celsius overnight. The following day cells were again centrifuged and freshly prepared fixative was added. The suspension was dropped onto a slide and ignited by an alcohol burner and allowed to flame. Slides were stained with 5% Giemsa solution. The preparations were examined by microscopy. The chromosomes of each cell were counted and only diploid cells were analyzed. They were scored for chromatid gaps and breaks, chromosome gaps and breaks, reunions, cells with greater than ten aberrations, polyploidy, pulverization, and other chromosomal aberrations which were observed. Fifty metaphase spreads were scored per animal. Mitotic indices were obtained by counting at least 500 cells and the ratio of the number of cells in mitosis / the number of cells observed was expressed as the mitotic index. Negative and positive (TEM) controls were run in each experiment. Two tests were performed at different time intervals.
- GLP compliance:
- not specified
- Remarks:
- pre-GLP
- Type of assay:
- chromosome aberration assay
- Species:
- rat
- Strain:
- not specified
- Sex:
- male
- Route of administration:
- oral: gavage
- Duration of treatment / exposure:
- Acute study: single dosing; subacute study: once a day for 5 consecutive days
- Frequency of treatment:
- Acute study: single dosing; subacute study: once a day for 5 consecutive days
- Post exposure period:
- Animals were killed 6, 24 and 48 hours after a single administration in the acute study. In the subacute study 5 doses, 24 hours apart, were administered and animals were killed 6 hours after the last dose.
- Remarks:
- Doses / Concentrations:
Test 1: acute and subacute: 3.75, 37.5, 375 mg/kg bw/day
Test 2: acute 5000 mg/kg bw and subacute 2500 mg/kg bw/day - No. of animals per sex per dose:
- Groups of 5 treated/dose and 3 control male animals were used.
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Triethylenemelamine (TEM)
- Tissues and cell types examined:
- Bone marrow
- Details of tissue and slide preparation:
- DETAILS OF SLIDE PREPARATION:
Four hours after the last compound administration, and two hours prior to sacrifice, each animal was given 4 mg/kg bw of colcemid intraperitoneally in order to arrest the bone marrow cells in mitosis. The marrow "plug" was removed and aspirated into Hanks' balanced salt solution. The specimen were centrifuged and resuspended in hypotonic 0.5 % KCl. The specimens were placed in a 37 °C water bath in order to swell the cells. Following centrifugation the cells were resuspended in a fixative (3:1 absolute methanol : glacial acetic acid) and again centrifuged. Cells were resuspended and placed at 4 °C overnight. The following day cells were again centrifuged and freshly prepared fixative was added. The suspension was dropped onto a slide and ignited by an alcohol burner and allowed to flame. Slides were stained with 5 % Giemsa solution.
METHOD OF ANALYSIS:
The preparations were examined by microscopy. The chromosomes of each cell were counted and only diploid cells were analyzed. They were scored for chromatid gaps and breaks, chromosome gaps and breaks, reunions, cells with greater than ten aberrations, polyploidy, pulverization, and other chromosomal aberrations which were observed. Fifty metaphase spreads were scored per animal. Mitotic indices were obtained by counting at least 500 cells and the ratio of the number of cells in mitosis / the number of cells observed was expressed as the mitotic index.
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Reference
Test I (3.75, 37.5 and 375 mg/kg bw/day dosing):
Acute study: The negative control group cells contained no aberrations. The compound produced no aberrations except for one cell containing a break in the 6-hour sample of the intermediate dose level. The expected severe chromosomal damage was observed for the positive control group (triethylenemelamine treated animals). The mitotic indices were within normal limits. Negative and positive controls were functional.
Subacute study (5 days): The negative control group and the low level test group contained no aberration. The intermediate level contained one cell with a reunion and one cell that was polyploid. The highest level contained three cells with breaks and one fragment. These were considered to be within the normal limits of the historical negative controls of the laboratory. Negative control was functional. No positive control was performed.
Test 2:
Acute study: Adipic acid was administered at a single dose of 5000 mg/kg bw. The compound produced no aberrations except for 3 cells with polyploidy (2 in the 6-hour sample and 1 in the 24-hour). Neither the variety nor the number of these aberrations differed significantly from the negative controls (polyploidy observed in 4 cells). Negative and positive controls were functional.
Subacute study (5 days, 2500 mg/kg bw/day). Only 218 metaphases have been evaluated. The compound produced no aberrations except for 1 cell with polyploidy. Polyploidy was also observed in the negative control group. These are considered to be within the normal limits of the historical negative controls. Negative control was functional, no positive control.
In summary, adipic acid can be considered non-mutagenic in the cytogenetic test.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
The genotoxicity potential of the test article was assessed with data available for structurally close related compounds (sebacic acid, adipic acid).
AMES TEST
In an Ames test (Shimizu, 1985), no mutagenicity was observered after exposure of S. typhimurium TA 1535, TA 1537, TA 98, TA 1538, TA 100, and E.coli WP2 uvrA with up to 5000 µg/plate sebacic acid with or without metabolic activation. Sebacic Acid disolved in DMSO was tested using the preincubation method in duplicates. Vehicle controls and adequate positive control substances were used concurrently. No cytotoxicity was observed within the used concentration range. No significant increase in the numbers of revertant colonies was recorded for any of the bacterial strains with any dose of Sebacic Acid either with or without metabolic activation. In a second Ames test following OECD guideline 471 (Safepharm Laboratories Limited, 1992), similar results were obtained. In this assay, Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98 and TA100 were treated with Sebacic Acid by the Ames plate incorporation method at five dose levels, in triplicate, both with and without the addition of a rat liver homogenate metabolising system. The experiment was repeated on a separate day using fresh cultures of the bacterial strains and fresh chemical solutions. The solvent (acetone) control plates gave counts of revertant colonies within the normal range. All positive control chemicals produced marked increases in the number of revertant colonies, both with and without the metabolising system. Sebacic Acid caused no reduction in the growth of the bacterial lawn at any of the dose levels employed in all of the strains of Salmonella used. Sebacic Acid was, therefore, tested up to the maximum recommended dose level of 5000 pg/plate. No significant increase in the numbers of revertant colonies was recorded for any of the bacterial strains with any dose of Sebacic Acid either with or without metabolic activation. Sebacic Acid was found to be non-mutagenic under the conditions of this test.
CHROMOSOME ABERRATION IN VITRO
In a mammalian chromosome aberration test (Litton Bionetics, 1974, cited in OECD SIDS for dicarboxylic acid category, July 2001), no abberation was observed after exposure of human embryonic lung fibroblast cells (WI-38) with up to 200 mg/L adipic acid without metabolic activation. Cytotoxicity was observed at 400 mg/L. The positive and negative controls were functional.
GENE MUATATION MAMMALIAN CELLS
Adipic acid was investigated in an OECD TG 476 study in Chinese hamster V79 cells in the absence and in the presence of metabolic activator (S9) up to concentrations of 10 mM. No precipitation of the test item was noted in any experiment; no biological relevant growth inhibition was observed with and without metabolic activation. In both experiments no biologically relevant increase of mutants was found after treatment with the test item. DMBA and EMS were used as positive controls and showed distinct and biologically relevant effects in mutation frequencies. In conclusion, adipic acid is considered to be non-mutagenic in the HPRT locus using V79 cells.
CHROMOSOME ABERRATION IN VIVO
In a chromosome abberration test (Litton Bionetics, 1974) male rats were gavaged with adipic acid; either once with up to 5000 mg/kg bw or once per day on 5 consecutive days with up to 2500 mg/kg bw. 200 - 500 metaphase chromosomes of bone marrow cells per dose were scored for chromatid gaps and breaks, chromosome gaps and breaks, reunions, cells with more than 10 aberrations, polyploidy, pulverization and other chromosomal aberrations. The mitotic indices for all dose groups were considered to be within the normal limits of the controls and there was no evidence of chromosomal damage. The positive control groups, performed only during the acute studies, were functional.
Justification for classification or non-classification
The available experimental test data with the source substances sebacic acid or adipic acid are reliable and suitable for classification purposes under Regulation 1272/2008. In vitro results with sebacic acid were negative as well as in vitro and in vivo results with adipic acid. As a result the target substance is also not considered to be classified for genitic toxicity under Regulation (EC) No. 1272/2008, as amended for the ninth time in (EC) No. 2016/1179.
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