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EC number: 226-009-1 | CAS number: 5216-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin sensitizer
Key value for chemical safety assessment
Skin sensitisation
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (sensitising)
- Additional information:
The authors of this study report conducted the assessment of Contact Hypersensitivity to the test substance in the Mouse (Local Lymph Node Assay). The study was carried out according to the OECD guideline n° 429 with no observed deviations or restrictions.
The test substance concentrations were selected for the main study were based on the results of a preliminary study.
In the main study, three experimental groups of five female/J mice were treated with test substance concentrations of 1, 5 or 10% w/w on three consecutive days, by open application on the ears. Five vehicle control animals were similarly treated, but with vehicle alone (Acetone/Olive oil (4:1 v/v)).
Three days after the last exposure, all animals were injected with3H-methyl thymidine and after five hours the draining (auricular) lymph nodes were excised and pooled for each animal.
After precipitating theof the lymph node cells, radioactivity measurements were performed. The activity was expressed as the number of Disintegrations Per Minute (DPM) and a stimulation index (SI) was subsequently calculated for each group.
No oedema was observed in any of the animals examined.
All nodes of the test substance treated animals were increased in size. The largest auricular lymph nodes were found in the higher dose groups
No macroscopic abnormalities of the surrounding area were noted in any of the animals.No macroscopic abnormalities of the surrounding area were noted.
Body weights and body weight gain of experimental animals remained in the same range as controls over the study period. The slight body weight loss, noted in some animals, was considered not toxicologically significant.
Mean DPM/animal values for the experimental groups treated with test substance concentrations 1, 5 and 10% were 8538, 18776 and 23082 DPM respectively. The mean DPM/animal value for the vehicle control group was 1182 DPM.
The SI values calculated for the substance concentrations 1, 5 and 10% were 7.2, 15.9 and 19.5 respectively. These results show that the test substance elicits an SI ≥ 3. The EC3 value (the estimated test substance concentration that will give a SI =3) was established to be between 0 and 1 %.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
The test substance was evaluated for its skin sensitisation properties according to the procedure described in the OECD guideline 429, i.e. the LLNA assay.
According to the CLP Regulation (EC 1272/2008), 3.4 Sensitiser, a substance is considered as skin sensitiser Category 1 in the LLNA assay if thecalculated stimulation index (SI) is three or more. I t can be thenallocated in one the the two sub-categories 1A and 1B as follows:
-sub-category 1A: Local lymph node assay, EC3 value ≤ 2 %
- sub-category 1B: Local lymph node assay, EC3 value > 2 %
The SI value calculated in the available study was > 3 and the EC3 value was established to be between 0 and 1 % therefore, the test substance is classified as skin sensitizer sub-category 1A.
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