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EC number: 218-377-7 | CAS number: 2136-89-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23-06-1981 to 24-06-1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412 Part 11
- Version / remarks:
- related to OECD Guidelline 202, Guideline 84/449/EWG (C2) and ISO 6341 but the incubaiton time is different (24 hrs vs 48hrs)
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- GLP not in force at the time of the study
Test material
- Reference substance name:
- α,α,α,4-tetrachlorotoluene
- EC Number:
- 226-009-1
- EC Name:
- α,α,α,4-tetrachlorotoluene
- Cas Number:
- 5216-25-1
- Molecular formula:
- C7H4Cl4
- IUPAC Name:
- 1-chloro-4-(trichloromethyl)benzene
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- A saturated solution preapred under hydrolytic conditions for the test-substance: A suspension of the substance in de-ionised water was adjusted to pH 12 with NaOH, stirred for 72 hours at 25C and filtered.
Analysis of stock solution: 304 mg DOC/L
concnertation of stock solution
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Analysis of stock solution: 304 mg DOC/L
concentration of stock solution 831.4 mg/L calculated fro DOC of the solution and carbon contnet ofthe substance of 36.57%
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: straus
- Source: own laboratory strain
- Age of parental stock (mean and range, SD): not given
- Feeding during test -not given
- Food type: algae (Scenedesmus supspicatus)
- Amount:
- Frequency: twice daily
Breeding: cultivated adult animals in glass vessels (4L) canging the medium once a week and feeding the animals twice a day. Medium : Synthetic water according to DIN 38 412 part 11
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Hardness:
- as per DIN 38 412 part 11
- Test temperature:
- 21 C thermostatically controlled
- pH:
- as per DIN 38 412 part 11
- Dissolved oxygen:
- not determined
- Salinity:
- as per DIN 38 412 part 11
- Conductivity:
- as per DIN 38 412 part 11
- Nominal and measured concentrations:
- Nominal concentrations: 8.31, 83.1, 103.9, 166.3, 415.7, 831.4 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100ml glass vessel
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 100mL
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 10 animals per vessel
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water according to DIN 38 412 part 11
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: conducted in dark
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : motility
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate 2mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 112 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 210 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- other: EC90
- Effect conc.:
- ca. 394 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- 70% mean immobilised daphnia following 24hr exposure to 2mg/L potassium dichromate
- Reported statistics and error estimates:
- EC values calculated by logarithmic regression of the measured data
EC10 95% CI = 42-131 mg/L standard error 104-118 mg/L
EC50 95% CI = 188-417 mg/L standard error = 202-221 mg/L
EC90 95% CI= 271-4203 mg/L standard error = 346-472 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Considering the incubation time of only 24 hours it cannot be excluded that a test according to OECD202 would result in a range of 10-100 mg/L for the EC50 value of the hydrolysed solution of the test substance to Daphnia magna
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