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EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 August 2004 to 06 January 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Commission Directive 87/302/EEC
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): incorporated into dry soil using reverse osmosis water and a Hobart A200N mixer
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Blades Biological, Cowden, Edenbridge, Kent, UK
ACCLIMATION
- Acclimation period: 24 hours - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20-21.1 °C
- pH:
- 6.0-6.4
- Moisture:
- 28-29 % (initiation); 23-26 % (termination)
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L glass beakers
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): industrial quartz sand (70 % w/w), kaolinite clay (20 % w/w), sphagnum moss peat (10 % w/w)
- Maximum water holding capacity (in % dry weight): 30 % is considered the appropriate maximum moisture content
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 681-702 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): weights recorded at day 0 before addition of test material or control and day 14 (surviving animals); mortalities and sublethal effects were recorded at day 14
VEHICLE CONTROL PERFORMED: no
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1.0, 10, 100 and 1000 mg/kg
- Results used to determine the conditions for the definitive study: yes - Nominal and measured concentrations:
- 0, 100, 180, 320, 560 and 1000 mg/kg
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 270 other: mg/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 % C.I. 250-290 mg/kg
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 180 other: mg/kg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Cumulative mortality data in the definitive test are presented in Table 1 whereas the mean earthworm weights are presented in Table 2.
- Results with reference substance (positive control):
- The results of the positive control study gave a 14-Day LC50 for chloroacetamide of 28 mg/kg with 95 % confidence limits of 26-30 mg/kg confirming the sensitivity of the test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the test, the 14 day LC50 of the test material to earthworms was 270 mg/kg (95 % C.I. 250-290 mg/kg). The NOEC was determined to be 180 mg/kg.
- Executive summary:
In a GLP compliant acute earthworm toxicity study conducted in line with standardised guideline OECD 207, the acute toxicity to earthworm of the test material over 14 days was determined. Earthworms were exposed to the test material at nominal test concentrations of 0, 100, 180, 320, 560 and 1000 mg/kg. Under the conditions of the test, the 14 day EC50 based on mortality was determined to be 28 mg/kg (95 % C.I. 250-290 mg/kg). The NOEC was determined to be 180 mg/kg.
Reference
Table 1: Cumulative mortality data
Cumulative mortality (initial population: 40) | % mortality | |||
Concentration (mg/kg) | Day 7 | Day 14 | Day 7 | Day 14 |
Control | 0 | 0 | 0 | 0 |
100 | 0 | 0 | 0 | 0 |
180 | 0 | 0 | 0 | 0 |
320 | 26 | 32 | 65 | 80 |
560 | 40 | 40 | 100 | 100 |
1000 | 40 | 40 | 100 | 100 |
Table 2: Mean earthworm weights
Mean earthworm weights (g) | ||||
Concentration (mg/kg) | Day 0 | Day 14 | ||
Control | 0.45 ± 0.090 | 0.38 ± 0.082 | ||
100 | 0.46 ± 0.084 | 0.41 ± 0.073 | ||
180 | 0.44 ± 0.096 | 0.38 ± 0.089 | ||
320 | 0.42 ± 0.090 | 0.29 ± 0.074 | ||
560 | 0.42 ± 0.079 | 100% mortaity | ||
1000 | 0.40 ± 0.078 | 100% mortality |
Description of key information
The 14 day EC50 of the test material was determined to be 270 mg/kg and the NOEC determined to be 180 mg/kg according to a study performed in line with OECD Guideline 207.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 270 mg/kg soil dw
Additional information
In a GLP compliant acute earthworm toxicity study conducted in line with standardised guideline OECD 207, the acute toxicity to earthworm of the test material over 14 days was determined. Earthworms were exposed to the test material at nominal test concentrations of 0, 100, 180, 320, 560 and 1000 mg/kg. Under the conditions of the test, the 14 day EC50 based on mortality was determined to be 28 mg/kg (95% C.I. 250-290 mg/kg). The NOEC was determined to be 180 mg/kg.
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