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EC number: 213-947-1 | CAS number: 1068-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-01-24 to 2013-01-24
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- , adopted 4 April 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "The Guidelines for the Testing of Chemicals" State Environmental Protection Administration (SEPA) of China, 2004, No.209, 'Activated Sludge, Respiration Inhibition Test'
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- Chloro(1-methylethyl)magnesium
- EC Number:
- 213-947-1
- EC Name:
- Chloro(1-methylethyl)magnesium
- Cas Number:
- 1068-55-9
- Molecular formula:
- C3H7ClMg
- IUPAC Name:
- chloro(1-methylethyl)magnesium
- Reference substance name:
- Isopropylmagnesium chloride
- IUPAC Name:
- Isopropylmagnesium chloride
- Test material form:
- other: liquid: 20% in Tetrahydrofuran
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- 16 mL of synthetic sewage feed and specific weight of test item were made up to 300 mL with Tap water (aerating for 24 hours). 200 mL of microbial inoculation liquid was added and the total mixture (500 mL) poured in to the vessel
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - 4 g/L activated sludge (pH = 7.49) was prepared as microbial inocluation liquid when it was used
- activated sludge streamd from Guizhou estate water Co., LTD
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- Temperature range 19.5 °C - 19.7°C
- pH:
- pH range 7.59 - 7.72
- Dissolved oxygen:
- Dissolved oxygen range 3.28 - 6.50
- Nominal and measured concentrations:
- Nominal concentrations:
25,0 mg/L, 50.0 mg/L, 100 mg/L, 200 mg/L, 400 mg/L - Details on test conditions:
- Observed testconcentrations were made up in 15-min intervalls to give a series of vessels containing different concnetrations of test substance. The contact time in vessels was three hours. Subsequently the respiration rate of 10 minutes was meassured in each vessel.
- Temperature 19.2 °C
- Relative humidity 43% - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- LC50 (3h) = 10.6 mg/L
This is in accordance with the accepted 5 - 30 mg/L range, which satisfied the test requirement. The results of reference substance are valid. - Reported statistics and error estimates:
- The respiration rate was calculated from the recorder trace as mg O2/L. In order to calculate the inhibitory effet of a test substance the repration rate was expressed as a percentage of the mean of the two control respiration rates.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the performed Activated Sludge Respiration Inhibition Test, the EC50 value of test item was determined as higher than 400 mg/L.
- Executive summary:
The acitvated sludge respiration inhibition test of isopropyl magnesium chloride was conducted. Therefore test item was given to activated sludge for 3 hours. Subsequently the respiration rate of 10 minutes was measured. The respiration rate was calculated from the recorder trace as mg O2/L. In order to calculate the inhibitory effet of a test substance the respiration rate was expressed as a percentage of the mean of the two control respiration rates. Corresponding to the observed concentrations (25, 50, 100, 200 and 400 mg/L isopropyl magnesium chloride) the respiration depression were 5.0%, 12.6%, 15.4%, 19.5% and 24.2%. The result showed that EC50 was greater than 400 mg/L. 3,5 -Dichlorphenol was used as positive control. The calculated EC50 (3 hours) = 10.6 mg/L and was in the accepted range from 5 to 30 mg/L.
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