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EC number: 213-947-1 | CAS number: 1068-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-11-20 to 2013-06-14
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: "The Guidelines for the Testing of Chemicals" State Environmental Protection Administration (SEPA) of China, No.202
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- Chloro(1-methylethyl)magnesium
- EC Number:
- 213-947-1
- EC Name:
- Chloro(1-methylethyl)magnesium
- Cas Number:
- 1068-55-9
- Molecular formula:
- C3H7ClMg
- IUPAC Name:
- chloro(1-methylethyl)magnesium
- Reference substance name:
- Isopropylmagnesium chloride
- IUPAC Name:
- Isopropylmagnesium chloride
- Test material form:
- other: 20% in 80% Tetrahydrofuran
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Testconcentration was messeaured at beginning and the end of the test c(48h)/c(0h) = 96.8%
Test solutions
- Details on test solutions:
- Preparation of stock solution (100 mg/L Mg) and nominal tested concnentrations:
250 µL Isopropyl magnesium chloride ca. 20% in Tetrahydrofuranwas added into a 500 mL beaker (contained 50 mL pure water). 500 mL test water was added and treated with ultrasonic dissolving for 20 min. Thereafter, after filtrated through 0.45 µn filter membrane, 1 mol/L HNO3 was added to adjust the pH value to 7.8 +- 0.1 and it was stirred with a glass rod for 2 min to obtain 100 mg/L test solution of Isopropyl magnesium chloride ca. 20% in Tetrahydrofuran.
Reference soluiton: Deionized water
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: Shanghai Fisheries university school of life science and technology
- Age at study initiation: 0-24 h (small Daphnia were removed), more than second generation
ACCLIMATION
- Temperature: 20°C
- Culturing avoided from direct sunlight
- Type and amount of food: cultivated algae of the lab, daily
- Health during acclimation (any mortality observed): if no mortality occured during 24 hours Daphnia were used for the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- Temperature 20.1°C to 20.6°C at the start of the test
Temperature 19.8°C to 20.7°C at the end of the test - pH:
- pH 7.82 to 7.85 at the start of the test
pH 7.81 to 7.85 at the end of the test - Dissolved oxygen:
- 108.1 to 110.5 mg/L at test start of the test
94.9 mg/L to 97.5 mg/L at test end of the test - Nominal and measured concentrations:
- Nominal concentration: 100 mg/L (limited test); it was not necessary to conduct the defiitve test , because Daphnia magna showed no effects during exposition. Nominal concentration is equal to analytical measured concentration of 116,89 mg/L.
The concentrations were measured by the described analytical method. - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: five
- No. of vessels per concentration (replicates): four replicates
- No. of vessels per control (replicates): four replicates
- No. of vessels per vehicle control (replicates): four replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- 11.76 g CaCl2 dissolved in deionized water, diluted to 1 L deionized water
- 2.59 g NaHCO3 dissolved in deionized water, diluted to 1 L deionized water
- 0.23 g KCl issolved in deionized water, diluted to 1 L deionized water
- 25 mL of these solutions were mixed and diluted in 1L deionized water
- after 30 min of aearation ph, dissolved oxygen were measured (after 48h test duration again)
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Adjustment of pH: 1 mol/L HNO3 to adjust the pH value to 7.8 +- 0.1
EFFECT PARAMETER MEASURED:
- Inhibition of Daphnia after 24h and 48h
- EC50 (48h)
TEST CONCENTRATIONS:
Preliminary test:
- nominal concentrations: 1, 10, 100 mg/l + control group (five Daphnia magna pro group)
- Expositiontime: 48 hours
- Pretest results showed that the inhibiton rate to Daphnia was 0 at 100 mg/L (higest tested concentration) - tested concentrations: 1, 10, 100 m/L
- Therefore it was not necessary to conduct the definitve test
- EC50 (48h) was determinded with limited testconcentration of 100 mg/L
- Testconcentration was messeaured at beginning end end of the test c(48h)/c(0h) = 96.8% - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromat
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 116.89 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 (24h) = 0.9 mg/L
This 24-hour EC50 value is in accordance with the accepted 0.6 – 2.1 mg/L range for Daphnia magna. - Reported statistics and error estimates:
- No calculation was necessary, because under exposition at highest test concentration no effects were observed.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Daphnia magna did not have abnormal behavior/mobility during the test period. The result indicated that the EC50(48h) for Daphnia magna was greater than 100 mg/L (highest observed testconcentration).
- Executive summary:
The purpose of this study was to evaluate the influence of the test item isopropyl magnesium chloride on the mobility of Daphnia magna. Young organims were exposed to testwater at a range of test item concetrations for 48 h.
Atomic absorption spectrometry of test solutions was used to generate standard curve. This chromotographic genereated curve enabled to read substance concentration directly at the beginning and the end of test ( c(48h)/c(0h) = 96.8%).
During the pretest the testsolution indicated no effets on Daphnia magna under nominal concentrations of 1, 10 and 100 mg/L isopropyl magnesium chlorid. Therefore it was not necessary to conduct the definitve test. However limit test with 100 mg/L testsolution was carried out for four replicates (five organisms). Each organism was less than 24 h old and more than the second generation. The inhibition of Daphnia magna was recorded after 24 h and 48 h test duration. The result indicated that the EC50(48h) for Daphnia magna was greater than 100 mg/L, which was highest observed nominal testconcentration and and was equivalent to 116,89 mg/L (analytical meassured result). Potassium dichromat was used as positive control. The calculated EC50 (24h) = 0.9 mg/L is in accordance with the accepted 0.6 – 2.1 mg/L range for Daphnia magna.
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