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The acute toxicity of isodecyl benzoate as measured by mortality to rainbow trout was evaluated in freshwater. The LC50 for the test material was determined to be greater than 6.5 mg/L, and the highest concentration achievable under the conditions of the test. The 96 -hour no mortality concentration and no observed effect concentration were also determined to be 6.5 mg/L.

The acute toxicity of isodecyl benzoate as measured by immobility to the water flea (Daphnia magna) was evaluated in freshwater. The test material did not produce acute toxicity to Daphnia at a loading level of 108 mg/L which measured 0.014 mg/L, the highest concentration achievable under the conditions of the test.

The toxicity of isodecyl benzoate as measured by biomass and growth rate to the green algae (Selenastrum capriornutum) was evaluated in fresthwater. No significant effect was observed at the maximum test material water solubility which measured 50 ug/L under the conditions of this study.

The chronic toxicity of the test material as measured by hatching success, survival, and growth to the fathead minnow was evalutated in freshwater. No effects were observed under the conditions of the test. The no observed effect concentration was 47 ug/L (mean measured concentration), the highest concentration achievable under the conditions of the test. The nominal loading was 100 ug/L.

The chronic toxicity of isodecyl benzoate as measured by reproduction, survival and growth of the water flea (Daphnia magna) was evaluated in freshwater. No significant effect was observed at the maximum test material water solubility under the conditions of the test, which measured 39 ug/L. The test material did not affect reproductive capacity in Daphnia magna at the maximum water soluble concentration achievable under the test conditions