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Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992-12-11 to 1993-01-18
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
yes (incl. QA statement)
Radiolabelling:
no
Details on sampling:
Samples of water were analysed two times per week (single samples).
Samples of fish were analysed at week 2, 4 , 6 and 8 (duplicate samples).
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
Two concentrations were prepared in dilution water. The nominal concentrations were 0.5 mg/l and 0.05 mg/l. A dispersant (HCO-40) was used in the test (the actual concentration present is unclear but appears to have been around 20 times the volume of test substance). A control containing HCO-40 was also run.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
At test initiation the fish had an average weight of 22.0 g, an average length of 9.3 cm and an average lipid content of 3.9%.TEST ORGANISM
- Common name: Carp
- Source: Sugishima fish farm (Address: 123-2 Gunchiku Ichibancho, Yatsushiro-shi, Kumamoto 866, Japan), Date received September 8, 1992
Weight, length and lipid content at initial exposure (Lot No. TFC-920908)
Weight average 22.0 g
Length average 9.3 cm
Lipid content average 3.9%

The fish were checked visually at the delivery and removed those demonstrating any abnormalities. The fish were reared for 1 day in a flow through system following an external disinfection. The disinfection was carried out by use of 50 mg/L Terramycin
(Taito pfizer) and 7 gIL Sodium Chloride for 24 hours under a static water condition.

ACCLIMATION
After rearing, the fish were medicated to eliminate parasites and transferred to an acclimatizing aquarium. After the second external disinfection, they were acclimatized. The fish those demonstrating any abnormalities during this period were removed and
reared for 25 days in a flow through system at the temperature of 25 ± 2 DC. The fish were then transferred to test tanks and reared at the same temperature in the flow through system for another 37 days, following the external disinfection.

Date of completed acclimatization October 9, 1992.
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk
Total depuration duration:
0 wk
Hardness:
116 mg/l (Ca, Mg)
Test temperature:
25°C
pH:
No data
Dissolved oxygen:
6.4-7.9 mg/l
TOC:
TOC was not given. The chemical oxygen demand of the water was <0.5 mg/l.
Details on test conditions:
A flow-through test system was used. The test vessels were 100 litre glass tanks receiving 579 litres/day of test water. The number of fish in each exposure group was 15 at the start of the test and the exposure was carried out for 8 weeks. No depuration phase was included.

Test tank: Round glass vessel
Volume of test water: 4 L/level
Temperature of test water 25 ± 2°C
Concentration of dissolved oxygen in test water
At initial exposure 7.6 mg/L
At termination of exposure 5.9 - 7.2 mg/L
pH of test water
At initial exposure 7.9
At termination of exposure 7.8 - 8.0
Number of fish: 10/level
Duration of exposure: 48 h

Preparation of stock solution:


Nominal and measured concentrations:
The mean measured concentrations in the water were 0.489 mg/l at week 2, 0.469 mg/l at week 4, 0.470 mg/l at week 6 and 0.469 mg/l at week 8 in the nominal 0.5 mg/l treatment group and 0.0470 mg/l at week 2, 0.0470 mg/l at week 4, 0.0475 mg/l at week 6 and 0.0482 mg/l at week 8 in the nominal 0.05 mg/l treatment group.
Reference substance (positive control):
no
Details on estimation of bioconcentration:
The BCF was estimated based on the ratio of the measured concentration in the fish to the measured concentration in the water.
Lipid content:
4.1 %
Time point:
start of exposure
Key result
Temp.:
25 °C
pH:
7.9
Type:
BCF
Value:
< 8 dimensionless
Basis:
whole body w.w.
Remarks on result:
other: The concentration in fish was below the limit of detection in all fish. The BCF represents the upper limit based on the limit of detection.
Remarks:
Conc.in environment / dose:0.05 mg/l
Key result
Temp.:
25 °C
pH:
7.9
Type:
BCF
Value:
ca. 1.4 - ca. 2.6 dimensionless
Basis:
whole body w.w.
Remarks on result:
other: The BCF was in the range 1.4 to 2.6 l/kg over week 2, 4, 6 and 8.
Remarks:
Conc.in environment / dose:0.5 mg/l

Concentrations of test substance in test water

Table 1: Measured concentrations of test substance in test water (average value at times from start of exposure)

 

2 weeks

4 weeks

6 weeks

8 weeks

Level 1

0.489

0.469

0.470

0.469

Level 2

0.0470

0.0470

0.0475

0.0482

 

Table 2: BCFs

 

2 weeks

4 weeks

6 weeks

8 weeks

Level 1

2.2

2.0

1.9

2.3

2.6

2.3

1.4

1.4

Level 2

<8.0

<8.0

<8.0

<8.0

<8.0

<8.0

<8.0

<8.0

 

BCFs of the test substance were 1.4 to 2.6 for Level 1 and lower than 8.0 for Level 2.

Results of test fish observation, no abnormality in behaviour or appearance was noted.

Each average exposure level was maintained at higher than 94 % for both exposure levels of the nominated concentrations

Validity criteria fulfilled:
yes
Conclusions:
The BCF was determined to be in the range 1.4 -2.6 in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. The BCF is low.

Executive summary:

The BCF was determined to be in the range 1.4 -2.6. The BCF is low.

The substance tested is the likely hydrolysis product of DCPMS.

Description of key information

Bioaccumulation: aquatic: The BCF for the hydrolysis product has been determined to be in the range 1.4-2.6. The BCF is low (OECD 305).

Key value for chemical safety assessment

Additional information

A reliable study conducted according to OECD Test Guideline 305C and according to GLP is available. The test substance was dicyclopentylsilanediol, the silanol hydrolysis product of dicyclopentyl(dimethoxy)silane. The measured BCF is low. The BCF was in the range 1.4 to 2.6 over 2, 4, 6 and 8 weeks at the higher test concentration (0.5 mg/L). At the lower test concentration (0.05 mg/L), the concentration in fish was below the limit of detection in all fish; a BCF value of <0.8 represents the upper limit based on the limit of detection.

Based on use pattern, exposure to the aquatic compartment is unlikely to be high, and furthermore based on the hydrolysis half-life of 19 hours at pH 7, any exposure of the aquatic compartment would be predominantly to the hydrolysis products. Therefore, in accordance with Column 2 of REACH Annex IX, the bioaccumulation in aquatic species study (required in Section 9.3.2 of REACH Annex IX) does not need to be conducted for the parent substance.