2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report with restrictions (no data on test substance purity and body weight development, no GLP)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Reference 2

Reference Type:

other: Correspondence

Title:

Unnamed

Year:

1989

Report date:

1989

Reference 3

Reference Type:

publication

Title:

Unnamed

Year:

2001

Reference 4

Reference Type:

publication

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

(no data on test substance purity and body weight development)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol

EC Number:

247-384-8

EC Name:

2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol

Cas Number:

25973-55-1

Molecular formula:

C22H29N3O

IUPAC Name:

2-(2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylpropyl)phenol

Test animals

Species:

rat

Strain:

other: Tif: RAIf (SPF)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 160-180 g
- Fasting period before study: overnight
- Housing: in groups of 5 in Macrolon cages (type 3)
- Diet (ad libitum): rat food - NAFAG, Gossau SG
- Water (ad libitum)
- Acclimation period: a minimum of 4 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 1
- Humidity (%): 55 +/- 5
- Photoperiod (hrs dark / hrs light): 14/10

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 30 % of Formulation

DOSAGE PREPARATION: Before treatement the suspension was homogeneously dispersed with an Ultra-Turrax and during treatment it was kept stable with a magnetic stirrer.

Doses:

4640, 6000, 7750 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: Physical condition and rate of deaths were monitored throughout the whole observation period.
- Necropsy of survivors performed: yes (submitted at random to a necropsy at the end of the observation period)

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 7 750 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality occured.

Mortality:

No mortality occured.

Clinical signs:

other: Within 2 hours after treatment the rats in all dosage groups showed sedation, dyspnoe, curved position and ruffled fur. The animals recovered within 8 to 9 days.

Gross pathology:

No substance related gross organ changes were seen.