Registration Dossier

Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 January 2018 - 24 March 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
other: Test item was diluted with methanol

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: viscous
Details on test material:
Identification: m-bis(2,3-epoxypropoxy)benzene
EC Number: 202-987-5
Physical state/Appearance: colorless slightly viscous liquid
Batch: 53715080
Purity: not provided
Expiry Date: 05 Aug 2019
Storage Conditions: room temperature in the dark
Specific details on test material used for the study:
- Source and lot/batch No.of test material: 53715080
- Storage condition of test material: Room temperature in the dark
Radiolabelling:
no

Study design

Test temperature:
30 °C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
HPLC System : Agilent Technologies 1200 Series
Detector type : variable wavelength (VW)
Column : HSS CYANO 5 μm (150 x 4.6 mm id)
Column temperature : 30 °C
Mobile phase : Methanol: purified water (55:45 v/v)
pH of mobile phase : 5.7
Flow-rate : 1.0 mL/min
Injection volume : 10 μL
VW detector wavelength : 210 nm (standards and dead time)
220 nm (sample)
- Method: The dead time was determined by measuring the retention time of formamide (purity* 99.6%, 607 mg/L solution in methanol: purified water 55:45 v/v) REPETITIONS
- Number of determinations: The sample, dead time and reference standard solutions were injected in duplicate
EVALUATION
- Calculation of capacity factors k': Equation 18 in report
- Calculation of retention times: log k' versus log Koc of the reference standards was plotted using linear regression
- Determination of the log Koc value: equation 19 in report

Batch equilibrium or other method

Analytical monitoring:
yes
Details on sampling:
- Concentrations: Test item (0.1022 g) was diluted to 100 mL with methanol to give a concentration of 1.02 g/L.

Results and discussion

Adsorption coefficient
Key result
Type:
log Koc
Value:
ca. 1.65 dimensionless
pH:
5.7
Temp.:
30 °C

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The adsorption coefficient of the test item has been determined to be 45.1, log10 Koc 1.65. The result classified the test item as having very high mobility.