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EC number: 427-440-1 | CAS number: 24487-91-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 February 1997 - 21 February 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to GLP in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 427-440-1
- EC Name:
- -
- Cas Number:
- 24487-91-0
- Molecular formula:
- C9H9ClO2
- IUPAC Name:
- 2-Methyl-3-methoxy benzoyl chloride
- Test material form:
- other: solid
- Details on test material:
- MMBC
Appearance: Light brown solid
Storage conditions: In darkness at room temperature
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
The test substance rapidly hydrolysed to MMBA.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- TEST SUBSTANCE PREPARATION
The test substance was added to diluent water to give an initial stock solution of 199.2 mg test substance equivalents/L and ultrasonicated for approximately 90 minutes so that test substance dissolved by hydrolysis to MMBA. The pH of this stock solution was then adjusted using 5.5 mL 1N sodium hydroxide to a pH of 7.8.
Serial dilutions of this stock solution were prepared with test water to give the desired series of exposure levels.
STABILITY OF TEST CONCENTRATIONS
Test concentrations were verified by chemical analysis. Duplicate medium samples (10 mL) were taken from the control and each exposure level at 0 hours (fresh media), 24 and 96 hours (expired media) and analysed.
CONCENTRATIONS
Five test concentrations were prepared plus one control.
Test substance rapidly hydrolysed
to MMBA with a half life of <15 minutes at 25°C in water. Based on this data, the compound in this study was initially converted to the hydrolysis product MMBA in order to test the compound up to a concentration of 100 mg/L as required in the pertinent guidelines. The method of analysis investigated the concentration of MMBA, however all results are expressed as mean measured concentration of MMBC equivalents. Measured concentrations ranged from 100 to 110% of nominal at 0 hours, 101 to 108% of nominal at 24 hours, and 105 to 112% of nominal at 96 hours.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Norfolk, UK.
- Age at study initiation (mean and range, SD): Not reported.
- Length at study initiation (length definition, mean, range and SD): The control fish were measured at the end of the exposure period. Mean standard length was found to be 3.2 cm (SD = 0.32).
- Weight at study initiation (mean and range, SD): The control fish were measured at the end of the exposure period. Mean weight was determined as 0.86 g (SD = 0.14).
- Feeding during test: No
ACCLIMATION
- Acclimation period: 13 days.
- Acclimation conditions (same as test or not): Temperature remained within the range 14 to 15 °C and dissolved oxygen within the range 9.5 to 10.1 mgO2/L.
- Type and amount of food: Commercial fry pellet, fed to repletion. No food was given during the 24 hour period immediately prior to exposure.
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): No medication was given during the acclimatisation period and mortalities were recorded as <1% in the 7 days prior to the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 136 - 181 mg CaCO3/L, calculated daily.
- Test temperature:
- 13 ± 1 °C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 9.1 - 10.2
- Nominal and measured concentrations:
- Nominal test concentrations: 4.58, 9.96, 21.9, 45.8 and 99.6 mg test substance equivalents/L.
Mean measured test concentrations: 5.06, 10.8, 23.5, 46,8 and 103 mg test substance equivalents/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria containing prepared test medium or diluent water.
- Material, size, headspace, fill volume: Each vessel contained 20 litres of medium to a depth of 19 cm (approximate dimensions of vessel: 25 x 46 x 25 cm).
- Aeration: Supplementary aeration was provided via a narrow bore glass.
- No. of organisms per vessel: 10
- Biomass loading rate: 0.43 g bodyweight/litre (static volume).
DILUENT WATER
The water used for the study was laboratory tap water filtered, dechlorinated and softened by passage through an Elga water purification system. The water passed in sequence through a high pressure sand filter to remove colloids and particulate matter and a high grade activated carbon filter to remove chlorine and any organic contaminants. A proportion of the supply then passed through a water softener before final reverse osmosis treatment to produce a highly purified water supply. The two grades of dechlorinated water were then remixed in the ratio of approximately 1 : 1 to give a supply with the desired water hardness.
Chlorine levels ranged from 0.00 to 0.09 mg/L in the seven days of the acclimatisation period and the mean hardness level, calculated from daily measurements during the same period, was between 136 and 181 mg CaCO3/L.
The water was analysed and the levels of dissolved minerals, metals and organics was found to be not significant.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness.
CRITERIA OF EFFECT
The criteria of death employed were (i) absence of respiratory movement and (ii) absence of response to physical stimulation of the caudal peduncle.
In addition to observations on mortality at 3, 6, 24, 48, 72 and 96 hours, subjective assessments were also made on the type and incidence of sub-lethal effects compared with control fish.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 103 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: MMBC equivalents as the test material hydrolyses rapidly to MMBA
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 103 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: MMBC equivalents as the test material hydrolyses rapidly to MMBA
- Basis for effect:
- mortality (fish)
- Details on results:
- There were no mortalities observed in any of the test levels.
In addition, no sub-lethal effects were observed in any of the fish at any concentration.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1 Summary of Chemical Analysis Results
Nominal Concentration (mg/L)
Measured Concentration
QC Samples % Recovery
As MMBA (mg/L)
As MMBC (mg/L)
As a % of Nominal
0 hours
Control
4.6
10
22
46
100
ND
4.518
9.558
20.95
41.03
92.30
-
5.042
10.67
23.37
45.78
103.0
-
110
107
106
99.5
103
-
99.7
98.3
90.4
24 hours
Control
4.6
10
22
46
100
ND
4.471
9.594
20.63
41.58
90.96
-
4.989
10.71
23.02
46.40
101.5
-
108
107
105
101
102
-
99.1
99.6
89.8
96 hours
Control
4.6
10
22
46
100
ND
4.616
9.933
21.55
43.17
94.47
-
5.151
11.08
24.04
48.17
105.4
-
112
111
109
105
105
-
103
102
93.0
ND = None Detected
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 value for the test substance with rainbow trout was >103 mg test substance equivalents/L.
- Executive summary:
The acute toxicity of the test substance to fish was investigated in Oncorhynchus mykiss (rainbow trout) in accordance with standardised guidelines OECD 203 and EU Method C.1. Groups of ten fish were exposed to hydrolysed test substance for 96 hours at nominal concentrations of 4.58, 9.96, 21.9, 45.8 and 99.6 mg test substance equivalents/L dissolved in water (mean measured concentrations of 5.06, 10.8, 23.5, 46.8 and 103 mg test substance equivalents/L). Since the test substance was seen to convert to the hydrolysis product MMBA, the method of analysis investigated the concentration of MMBA, however, all the results were expressed as mean measured concentration of test substance equivalents. During the study, observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 3, 6, 24, 48, 72 and 96 hours exposure. No mortalities or sub-lethal effects were observed, therefore the 96 hour LC50 value was estimated to be greater than 104 mg test substance equivalents/L.
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