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EC number: 269-824-8 | CAS number: 68334-33-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- April 19, 1990 - February 07, 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Although this study is guideline and GLP compliant and would normally be assigned a reliability of 1 (reliable without restrictions), this study is used for read-across and therefore has been assigned a reliability of 2 (reliable with restrictions).
- Justification for type of information:
- The primary component of all substances provide complete coverage of 68334-33-8. 68334-33-8 shares high structural similarity with 61789-80-8, 68783-78-8, 107-64-2, and 112-02-7. As 68334-33-8 is a UVCB its components encapsulate the other substances except for the counter ion (Cl-). In solution, the counter ions will dissociate from the parent structures. Therefore, we are comparing substances of equivalent nature. CAS 107-64-2 represents the C18 boundary of the 61789-80-8. Ignoring the salt component CAS 61789-80-8 is equivalent to CAS 68334-33-8. 68783-78-8 is a worst case of both 68334-33-8 and 61789-80-8 since it is unsaturated and the SP2 carbon-carbon bonds are of higher reactivity and a more likely site of metabolic activation. The primary component of CAS 112-02-7 is a substructure of all the other substances. Additional documentation, provided within the IUCLID Assessment Reports section, supports the read-across approach.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- The oxygen depletion in the bottle without inoculation exceeds the values laid down in the guide lines slightly.
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- Secondary activated sludge was obtained from a plant treating predominately domestic wastewater. The sludge was pre-conditioned to reduce the endogenous respiration rates. To precondition, the sludge (200 mg Dry Weight (DW)/litre) was aerated for a period of one week. The sludge was diluted to a concentration in the BOD bottles of 2 mg DW/litre. Ammonium chloride was omitted from the medium to prevent nitrification. Due to omission the pH of the medium decreased slightly.
- Duration of test (contact time):
- >= 0 - <= 287 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- BOD:ThOD ratio
- Details on study design:
- TEST CONDITIONS
The sodium acetate was added to the bottles using a stock solution of 1.0 g/litre. A stock suspension of the test substance of 1.0 g/litre was prepared by ultrasonic dispersion. The sludge was diluted to a concentration in the BOD bottles of 2 mg DW/litre. Ammonium chloride was omitted from the medium to prevent nitrification. Due to omission the pH of the medium decreased slightly.
TEST SYSTEM
- Culturing apparatus: The test was performed in 280 ml BOD (Biological Oxygen Demand) bottles.
STATISTICAL METHODS:
The biodegradation was calculated as the ratio of the biochemical oxygen demand (BOD) to the theoretical oxygen demand (ThOD). The ThOD of the test compound is 2.6 g O2/g test substance. The BOD of the test substance and sodium acetate were calculated from the oxygen concentrations in the bottles without and with test substance (B, C) and the bottles without and with sodium acetate (B, D). - Reference substance:
- acetic acid, sodium salt
- Remarks:
- Lot number: B02099
- Parameter:
- % degradation (O2 consumption)
- Value:
- 3
- Sampling time:
- 38 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 287 d
- Details on results:
- DHTDMAC A4M is not biodegraded in the closed bottle test (28 days). However, in the prolonged closed bottle test DHTDMAC A4 M is biodegraded 68 % at Day 287. The pH of the medium at day 28 is 6.9.
- Results with reference substance:
- The validity of the test is shown by the oxygen consumption in the control bottle with sodium acetate and an endogenous respiration of 0.4 g/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- The test is valid as shown by an endogenous respiration of 0.4 g/L and the total mineralization of the reference compound, sodium acetate.
- Interpretation of results:
- other: Not readily biodegradable
- Conclusions:
- DHTDMAC A4M is not readily biodegradable.
- Executive summary:
Secondary activated sludge was obtained from a plant treating predominately domestic wastewater. The sludge was pre-conditioned to reduce the endogenous respiration rates. To precondition, the sludge (200 mg Dry Weight (DW)/litre) was aerated for a period of one week. The sludge was diluted to a concentration in the BOD bottles of 2 mg DW/litre. Ammonium chloride was omitted from the medium to prevent nitrification. Due to omission the pH of the medium decreased slightly.
DHTDMAC A4M was not biodegraded in the closed bottle test after 28 days (3%). However, in the prolonged closed bottle test DHTDMAC A4 M is biodegraded 68% at Day 287. The pH of the medium at day 28 is 6.9.
Reference
DHTDMAC A4M is not biodegraded in the closed bottle test (28 days). However, in the prolonged closed bottle test DHTDMAC A4 M is biodegraded 68 % at Day 287. The pH of the medium at day 28 is 6.9.
Description of key information
Based on 3% degradation with the read-across substance, the test substance is predicted to be not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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