Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 Oct 2017 - 23 Nov 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Version / remarks:
2008
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
Version / remarks:
1995
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 830.7550 (Partition Coefficient, n-octanol / H2O, Shake Flask Method)
Version / remarks:
1996
Deviations:
no
GLP compliance:
yes
Type of method:
flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Details on test material:
- Appearance: White powder
- Storage condition of test material: At room temperature

Study design

Analytical method:
other: UPLC-UV

Results and discussion

Partition coefficientopen allclose all
Key result
Type:
log Pow
Partition coefficient:
-0.6
Temp.:
20 °C
pH:
7
Key result
Type:
Pow
Partition coefficient:
0.28
Temp.:
20 °C
pH:
7
Details on results:
- Concentration analysed in the stock solution was 0.996 g/L.
- Nominal quantity of the substance in each test sample: 1.99 mg.
- Recovery (range) for the test samples: 99% - 105%.
- The log Pow values were within the criterion range of ± 0.3 log units (see table below)

Any other information on results incl. tables

Table: Shake-flask results

Ratio
n-octanol : buffer pH 7 [v:v]

Analyzed concentration

Pow

log Pow

pH

n-octanol
[g/L]

buffer pH 7
[g/L]

individual

mean

1:1

0.0744

0.261

2.8 x 10-1

2.8 x 10-1

-0.5

7.1

 

0.0726

0.260

2.8 x 10-1

 

-0.6

7.1

1:2

0.0627

0.228

2.7 x 10-1

2.7 x 10-1

-0.6

7.1

 

0.0614

0.231

2.7 x 10-1

 

-0.6

7.1

2:1

0.0870

0.321

2.7 x 10-1

2.7 x 10-1

-0.6

7.1

 

0.0900

0.328

2.7 x 10-1

 

-0.6

7.1

Mean

2.8 x 10-1

 

-0.6

 

Standard deviation

6.7 x 10-3

 

 

 

 

Applicant's summary and conclusion

Conclusions:
The log Pow of the substance at pH 7 and 20°C was determined to be -0.6.
Executive summary:

The log Pow of the substance was determined using the shake-flask method in a GLP study according to EC A.8, OECD 107 and OPPTS 830.7550. Quantitative analysis was performed by UPLC-UV. The partition coefficient was determined at 20°C and pH 7, obtaining a Pow of 0.28. The log Pow of the substance at pH 7 and 20°C was determined to be -0.6.