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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (e.g. no analytical verification of the test concentrations)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German Industrial Standard DIN 38412, part 15
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-methylacetamide
EC Number:
201-182-6
EC Name:
N-methylacetamide
Cas Number:
79-16-3
Molecular formula:
C3H7NO
IUPAC Name:
N-methylacetamide
Details on test material:
- test substance: N-Methylacetamide
- purity: 99 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
- test water: reconstituted freshwater was prepared from fully demineralized tap water, which was resalted by the addition of 294 mg/l CaCl2*2H2O, 123.3 mg/l MgSO4*7H2O, 64.8 mg/l NaHCO3, 5.8 mg/l KCl
- test water had a total hardness of 2.5 mmol/l, an acid capacity (to pH 4.3) of 0.8 mmol/l, 82 mg/l Ca, 12 mg/l Mg, > 8 mg/l oxygen, and ca. pH 8

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
- animal species: Leuciscus idus L., golden variety (golden orfe)
- mean body length: 5.3 cm (4.7-5.8 cm)
- mean body weight: 3.7 g (2.1-4.9 g)
- mean corpulence factor of the batch: 2.5
- duration of adaptation to test water and test temperature: 3 days

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Details on test conditions:
- volume of test water: 10 l
- test vessels: all-glass aquaria (30x22x24 cm)
- aeration: continuously with oil-free air
- No. of animals per test concentration: 10
- loading (fish/vessel): 3.7 g/l
- temperature: 20 °C +- 1 °C
- nominal test concentrations: 0.0 (control), 1000, 2150, 4640, and 10000 mg/l
- observations: mortality after 1, 4, 24, 48, 72, and 96 h; symptoms after 4, 24 and 48 h
- measurements: pH and oxygen content after 1, 24, 48, 72, and 96 h
- the median lethal concentration (LC50) was calculated using Probit Analysis (Finney DJ, Probit Analysis, Cambr. Univ. Press, 3rd Edition, 1971)

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3 390 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:
RS-Freetext:
Mortality:
----------
Nominal Cumulative mortality (n)
concentration (mg/l) 1 h 4 h 24 h 48 h 72 h 96 h
0 (Control) 0 0 0 0 0 0
1000 0 0 0 0 0 0
2150 0 0 1 3 3 4
4640 0 0 2 3 4 5
10000 0 0 7 9 10 10

Symptoms:
---------
Nominal Symptoms
concentration (mg/l) 1 h 4 h 24 h 48 h 72 h 96 h
0 (Control)
1000
2150 T
4640 T
10000 T N

Legend: T = tumbling; N = narcotic-like state


Oxygen:
-------
Nominal Oxygen content (mg/l)
concentration (mg/l) 0 h 24 h 48 h 72 h 96 h
0 (Control) 8.1 8.5 8.9 8.7 8.4
1000 8.2 8.6 8.7 8.7 8.6
2150 8.3 8.2 8.7 8.9 8.8
4640 8.0 8.0 8.9 8.9 8.7
10000 8.0 7.7 8.9 8.7 ---


pH:
---
Nominal pH
concentration (mg/l) 0 h 24 h 48 h 72 h 96 h
0 (Control) 8.0 7.8 8.0 8.7 8.4
1000 7.9 7.9 7.9 8.7 8.6
2150 7.8 7.8 7.9 8.9 8.8
4640 7.7 7.8 7.9 8.9 8.7
10000 7.7 7.7 8.0 8.7 ---


LC50
----
after 1 hour: > 10000 mg/l (1 % significance level)
after 4 hours: > 10000 mg/l (1 % significance level)
after 24 hours: 7240 mg/l (slope factor = 2.16)
after 48 hours: 4690 mg/l (slope factor = 2.21)
after 72 hours: 4600 mg/l
after 96 hours: 3390 mg/l (slope factor = 1.94)

NOEC: 1000 mg/l
LC0: 1000 mg/l
LC100: 10000 mg/l

The control with chloracetamide (48-h LC 50: ca. 26 mg/l)
corresponds to the normal sensitivity.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes