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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
31 March 1998 to 04 April 1998
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Concentration measurments only at t=0
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Deviations:
not specified
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
1992
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0.1 g/L
- Sampling method: The test material concentration was analysed at the beginning of the study
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test media (reconstituted water and test material) were freshly prepared. The calibrated flask was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated and stirred with a magnetic stirrer for 23 hours. After 24 hours the solution was filtered with a nutsch filter (pore size 10 to 16 µm).
- Controls: Reconstituted water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Strain: CRL/ZF 1
- Age at study initiation: Approximately 29 weeks

ACCLIMATION
- Acclimation period: 7 days
- Type and amount of food during acclimation: Tetra Min diet
- Feeding frequency during acclimation: Fish were fed during the acclimation, until one day before the start of the experiment.
- Health during acclimation: Less than 5% mortality in the test fish batch.

FEEDING DURING TEST: None
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
None
Hardness:
250 mg/L as CaCO3
Test temperature:
23°C
pH:
7.8 to 8.1
Dissolved oxygen:
92.2 to 97.7% oxygen saturation
Salinity:
n/a
Conductivity:
Not reported
Nominal and measured concentrations:
Nominal: 0.1 g/L
Measured (start of the test): 0.00074 g/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquarium
- Material, size, headspace, fill volume: 6 L test solution
- Aeration: No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 600 mL/fish

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fully demineralised water
- Culture medium different from test medium: No
- Intervals of water quality measurement: At the beginning of the exposure period and then daily.

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light, 12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The behaviour and general condition of all fish were checked immediately after the introduction into the test vessel, 3 and 6 hours later, and then daily.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 0.74 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 0.74 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 0.74 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.74 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: Signs of intoxication (slightly irregular swimming, near the water surface, near the bottom of the aquarium, lying on the back, gasping) were seen 3 hours after exposure up to the end of the study.
- Mortality of control: None
- Other adverse effects control: None

Table 1. Mortality results

 Concentration (mg/L)  Number of dead fish                  
   Immediately after test start  3 h  6 h  24 h  48 h  72 h  96 h
Control   0  0  0  0  0  0  0
 0.74  0  0  0  3  3  3  3
Validity criteria fulfilled:
not specified
Conclusions:
The 96-hour LC50 was > 0.74 mg/L for Brachydanio rerio.
Executive summary:

The acute toxicity of the test item to zebrafish (Brachydanio rerio) was determined in a 96 -hour static limit test conducted according to OECD Guideline 203 and EU Method C.2. Fish were exposed to a nominal concentration of 100 mg/L of the test item (measured concentration 0.74 mg/L) in reconstituted water, alongside a control group. The test solution was prepared by ultrasonication of the test item in the test media for 1 hour, followed by aeration and stirring for 23 hours. The solution was filtered before use. Each treatment contained one replicate with 10 fish. The 96 -h LC50 was determined to be > 0.74 mg/L. This test is reliable with restriction (Klimisch 2) as test concentrations were only measured at t = 0h.

Description of key information

Based on measured concentrations, the 96-hour LC50 was > 0.74 mg/L for Brachydanio rerio.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.74 mg/L

Additional information

The acute toxicity of the test item to zebrafish (Brachydanio rerio) was determined in a 96 -hour static limit test conducted according to OECD Guideline 203 and EU Method C.2 (1998). Fish were exposed to a nominal concentration of 100 mg/L of the test item (measured concentration 0.74 mg/L) in reconstituted water, alongside a control group. The test solution was prepared by ultrasonication of the test item in the test media for 1 hour, followed by aeration and stirring for 23 hours. The solution was filtered before use. Each treatment contained one replicate with 10 fish. The 96 -h LC50 was determined to be > 0.74 mg/L.