β,β-carotene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Fingerling rainbow trouts were exposed for 48 hours to different concentrations of the test substance at a water temperature of 14 ± 1 °C. The no-observed-effect-levels and lethal concentrations (LC100) were determined.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

β,β-carotene

EC Number:

230-636-6

EC Name:

β,β-carotene

Cas Number:

7235-40-7

Molecular formula:

C40H56

IUPAC Name:

β,β-carotene

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trouts
- Length at study initiation (length definition, mean, range and SD): 4 to 8 cm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The substance was defined as barely toxic (i.e. no toxic effects observed up to 1000 mg/L) on the basis of a 48-hour test on the acute toxicity to fish conducted with rainbow trouts.

Executive summary:

A study (presumably under static conditions) on the acute toxicity of beta-Carotene to rainbow trouts (Salmo gairdneri L., now Oncorhynchus mykiss) was conducted over a period 48 hours. Fingerlings of 4 to 8 cm body length were exposed to different concentrations of the test substance. The test temperature was 14 ± 1 °C. The substance was defined as barely toxic on the basis of the test results, i.e. no toxic effects were observed up to a (presumably nominal) test concentration of 1000 mg/L.