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EC number: 201-240-0
CAS number: 79-97-0
The study fulfilled the validity criteria laid down by the
guideline (Table 1).
Table 1: Validity criteria for OECD 202.
Criterion from the guideline
Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.
Immobilization was 0% in the control
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.
The dissolved oxygen concentration was ≥ 7.91 mg/L in the control and test vessels at the end of the test.
Results are summarized in Table 2. The derived EC50 (48 h) was 4.7
mg/L and the NOEC (48 h) was 1.7 mg/L (nominal).
Table 2. Immobility data after 0, 24 and 48 h.
Nominal test concentration
n° of replicates
n° of daphnids
immobility n° and % at
OECD guideline, GLP compliant study. Daphnia magna were exposed to the
test substance for 48 hours during the test. % of immobility and
abnormal behaviours were recorded.
48h EC50 value of 4.7 mg/L was calculated.
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