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EC number: 201-240-0
CAS number: 79-97-0
The validity criteria of the guideline are summarized below (Table 1).
Table 1: Validity criteria for OECD 301.
Criterion from the guideline
Validity criterion fulfilled
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%.
Mean degradation of the test item did not reach 20% within 28 d.
Criterion cannot be assessed.
Percentage degradation of the reference compound has reached the pass levels (60%) by day 14.
83.23% on Day 14
Oxygen depletion in the inoculum blank should not exceed 1.5 mg dissolved oxygen/L after 28 days.
Oxygen depletion in the inoculum blank was 1.31 mg dissolved oxygen/L after 28 d.
The residual concentration of oxygen in the test bottles should not fall below 0.5 mg/L at any time.
Residual concentrations were 6.42 mg/L.
Toxicity control: toxic, if % degradation < 25% within 14 d.
The percent degradation by the toxicity control was 22.58%.
The results are summarized in Table 2.
Table 2. Results - percent degradation
(based on ThOD)
OECD guideline, GLP-complaint study. The biodegradability of the
substance was assessed using the Closed Bottle Test. 5.99%
biodegradation occurred within the 28 day test period.
The test substance is not rapidly biodegradable under the conditions of
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