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EC number: 611-056-6 | CAS number: 538313-26-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- The water temperature dropped below 21°C (20.8°C), minimum recommended temperature, for a few hours (period corresponds to the extinction of the light), the second and third day of incubation.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certificate is included in the report
- Analytical monitoring:
- yes
- Details on sampling:
- Analyses were performed for the definitive test on controls and test solutions at T0, T24h, T48h (before and after renewal of test solutions), T72h and T96h
- Vehicle:
- no
- Details on test solutions:
- Available information has shown that the test item is not soluble in water.
A stock solution was prepared in the dilution water (nominal concentration: 500.0 mg/L), in the dark under magnetic stirring, one week before initiating the fish acute toxicity test.After membrane filtration (nylon membrane filter, 0.45 µm pore size, 90 mm diameter), the stock solution was diluted in the dilution water to achieve the required test concentrations. Most of the copper was undissolved and was removed by filtration. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Zebrafish Danio rerio (Hamilton,1822-Teleostei, Cyprinidae) obtained from a single stock held in test facility since November 23rd 2016.Supplier: EXOMARC (33310 Lormont, FRANCE)Holding:- Water: Dilution water NF EN ISO 6341 (continuous aeration and filtering device)- Feeding: TetraMin Tetra- Light: 14-hour light / 10-hour dark cycleBatch of fish used during the definitive test:Mean size: 2.8 cm ± 0.2 cmMean wet weight: 0.40 g ± 0.07 gNo mortality for the batch of fish before the start of the test.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Renewal of controls and test solutions after 48 hours
- Hardness:
- Not available
- Test temperature:
- Between 20.8 and 21.7°C
- pH:
- Controls: between 7.1 and 7.9
Test solutions: between 7.2 and 8.7 - Dissolved oxygen:
- Controls: between 5.9 and 8.9Test solutions: between 3.4 and 8.7
- Salinity:
- Not available
- Conductivity:
- of dilution water (reconstituted water - NF EN ISO 6341): 635 µS/cm
- Nominal and measured concentrations:
- 64.2 – 96.3 – 144.4 – 216.7 and 325.0 mg/L (initial nominal concentrations)
- Details on test conditions:
- Vessel: 5 litres glass vessels filled with 4 litresTest: 2 blank controls + 2 test vessels by concentrationNumber of animals: 8 for test concentrations and 8 for the controlsTest concentrations:Limit test: 326.5 mg/L (initial nominal concentration)Definitive test: 64.2 – 96.3 – 144.4 – 216.7 and 325.0 mg/L (initial nominal concentration)Biomass: 0.4 g/LFeeding: noneLight: 14-hour light / 10-hour dark cycleTemperature: between 20.8 and 21.7°C in a temperature-controlled roomAeration: noneRenewal: after 48 hours (test concentrations and controls)Duration: 96 hoursTimes of observation: 2 - 5 - 24 – 48 – 53 - 72 - 96 hoursToxic effect: Fish are considered dead if there is no visible movement (e.g. gill movements) and if touching of the caudal peduncle produces no reaction.
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 216.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- (initial nominal concentrations) corresponding to an exposure to a mixture of 5.4 µg/L dissolved copper and 78 mg/L 5,5’-bi-1H-tetrazole (diammonium salt).
- Details on results:
- The analyses performed during this study therefore confirmed that a certain amount of the test item is solubilized in the test medium after one week under magnetic stirring.
- Results with reference substance (positive control):
- Test result for the selected batch (test carried out from 11 January 2017 to 12 January 2017): LC50 24h = 191.7 mg/L (187.9 – 196.3)The LC50 24 hours was calculated using a logistic regression analysis with a bootstrap estimation of the 95% confidence limits.This value is included in the warning limits of the laboratory (mean LC50 2 SD) on this species corresponding to two standard deviations from the historic geometric mean of the median lethal concentration (141.6 – 259.7 mg/L). This result confirmed the appropriate sensitivity of the fish batch.
- Sublethal observations / clinical signs:
Limit test carried out on January 23, 2017
Exposure time Controls CuDABT
326.5 mg/L
(nominal concentration (mg/L))Test vessel 1 2 1 2 4h 0 0 4 4 Mortality after 4h (%)
0 0 100 100 pH ND ND 8.6 8.6 Dissolved oxygen (mg/L) ND ND 8.6 8.5 Definitive test:
Mortality observed within the test period:
Exposure
time
Controls
CuDABT
(nominal concentration (mg/L))64.2
96.3
144.4
216.7
325.0
2 h
0
0
0
0
0
0
0
0
0
0
4
4
5 h
0
0
0
0
0
0
0
0
1
0
/
/
24 h
0
0
0
0
0
0
0
0
2
1
/
/
48 h
0
0
0
0
0
0
0
0
2
1
/
/
53 h
0
0
0
0
0
0
0
0
2
2
/
/
72 h
0
0
0
0
0
0
0
0
2
2
/
/
96 h
0
0
0
0
0
0
0
0
2
2
/
/
Mortality after 96h (%)
0
0
0
0
50.0
100
pH measurements:
Exposure time
Controls
CuDABT
(nominal concentration (mg/L))64.2
96.3
144.4
216.7
325.0
0
7.9
7.9
8.5
8.5
8.6
8.6
8.6
8.6
8.7
8.7
8.7
8.7
24 h
7.2
7.2
7.5
7.5
7.7
7.7
8.0
8.0
8.3
8.3
8.6
8.6
48 h
7.2
7.2
7.3
7.3
7.3
7.3
7.6
7.6
8.2
8.0
/
/
48 h
(New test solution)
7.8
7.8
8.5
8.5
8.6
8.6
8.6
8.6
8.7
8.7
/
/
72 h
7.2
7.2
7.8
7.7
7.9
8.0
8.2
8.2
8.5
8.4
/
/
96 h
7.1
7.1
7.2
7.2
7.3
7.2
7.2
7.2
8.1
7.7
/
/
Dissolved oxygen measurements (mg/L):
Exposure time
Controls
CuDABT
(nominal concentration (mg/L))64.2
96.3
144.4
216.7
325.0
0
8.9
8.9
8.8
8.8
8.8
8.8
8.8
8.8
8.7
8.8
8.7
8.7
24 h
6.3
6.2
6.2
6.2
6.2
6.0
6.1
6.2
7.1
6.7
8.5
8.5
48 h
5.9
6.0
5.8
5.8
5.9
5.5
5.8
5.7
6.9
6.3
/
/
48 h
(New test solution)
8.9
8.9
8.9
8.9
8.9
8.9
8.8
8.8
8.8
8.8
/
/
72 h
6.8
6.8
6.8
6.7
6.6
5.7
6.2
6.1
7.3
7.1
/
/
96 h
6.1
6.2
5.5
5.6
5.5
4.0
3.4
3.7
5.4
4.8
/
/
- Validity criteria fulfilled:
- yes
- Remarks:
- The mortality observed in control vessels did not exceed 10% at end of the test. The dissolved oxygen concentration was at least 60% of air saturation value in control vessels throughout the test period. For other criteria see chapter "Overall remarks"
- Conclusions:
- In the test conditions, 50% fish mortality was observed after 96 hours of exposure to the concentration of CuDABT of 216.7 mg/L (initial nominal concentration).
- Executive summary:
In order to determine acute toxicity, the fish are exposed to the test item: CuDABT during a period of 96 hours.
Mortality is recorded at 24, 48, 72 and 96 hours.
Available information has shown that the test item is not soluble / poorly soluble in water and satured solution has been used. In the test conditions, 50% fish mortality was observed after 96 hours of exposure to the concentration of CuDABT of 216.7 mg/L (initial nominal concentration).
Reference
Description of key information
In the test conditions, 50% fish mortality was observed after 96 hours of exposure to the concentration of CuDABT of 216.7 mg/L (initial nominal concentration).
The LC50 is determined as 216.7 mg/L.
LC50 > 100 mg/L (nominal concentration of CuDABT)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 216.7 mg/L
Additional information
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